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Nucleic acid detection using BRET-beacons based on bioluminescent protein–DNA hybrids

Bioluminescent molecular beacons have been developed using a modular design approach that relies on BRET between the bright luciferase NanoLuc and a Cy3 acceptor. While classical molecular beacons are hampered by background fluorescence and scattering, these BRET-beacons allow detection of low pM co...

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Detalles Bibliográficos
Autores principales:	Engelen, Wouter, van de Wiel, Kayleigh M., Meijer, Lenny H. H., Saha, Bedabrata, Merkx, Maarten
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Royal Society of Chemistry 2017
Materias:	Chemistry
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5436041/ https://www.ncbi.nlm.nih.gov/pubmed/28217801 http://dx.doi.org/10.1039/c6cc10032e

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