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Control of type III protein secretion using a minimal genetic system
Gram-negative bacteria secrete proteins using a type III secretion system (T3SS), which functions as a needle-like molecular machine. The many proteins involved in T3SS construction are tightly regulated due to its role in pathogenesis and motility. Here, starting with the 35 kb Salmonella pathogeni...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5436071/ https://www.ncbi.nlm.nih.gov/pubmed/28485369 http://dx.doi.org/10.1038/ncomms14737 |
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author | Song, Miryoung Sukovich, David J. Ciccarelli, Luciano Mayr, Julia Fernandez-Rodriguez, Jesus Mirsky, Ethan A. Tucker, Alex C. Gordon, D. Benjamin Marlovits, Thomas C. Voigt, Christopher A. |
author_facet | Song, Miryoung Sukovich, David J. Ciccarelli, Luciano Mayr, Julia Fernandez-Rodriguez, Jesus Mirsky, Ethan A. Tucker, Alex C. Gordon, D. Benjamin Marlovits, Thomas C. Voigt, Christopher A. |
author_sort | Song, Miryoung |
collection | PubMed |
description | Gram-negative bacteria secrete proteins using a type III secretion system (T3SS), which functions as a needle-like molecular machine. The many proteins involved in T3SS construction are tightly regulated due to its role in pathogenesis and motility. Here, starting with the 35 kb Salmonella pathogenicity island 1 (SPI-1), we eliminated internal regulation and simplified the genetics by removing or recoding genes, scrambling gene order and replacing all non-coding DNA with synthetic genetic parts. This process results in a 16 kb cluster that shares no sequence identity, regulation or organizational principles with SPI-1. Building this simplified system led to the discovery of essential roles for an internal start site (SpaO) and small RNA (InvR). Further, it can be controlled using synthetic regulatory circuits, including under SPI-1 repressing conditions. This work reveals an incredible post-transcriptional robustness in T3SS assembly and aids its control as a tool in biotechnology. |
format | Online Article Text |
id | pubmed-5436071 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-54360712017-05-25 Control of type III protein secretion using a minimal genetic system Song, Miryoung Sukovich, David J. Ciccarelli, Luciano Mayr, Julia Fernandez-Rodriguez, Jesus Mirsky, Ethan A. Tucker, Alex C. Gordon, D. Benjamin Marlovits, Thomas C. Voigt, Christopher A. Nat Commun Article Gram-negative bacteria secrete proteins using a type III secretion system (T3SS), which functions as a needle-like molecular machine. The many proteins involved in T3SS construction are tightly regulated due to its role in pathogenesis and motility. Here, starting with the 35 kb Salmonella pathogenicity island 1 (SPI-1), we eliminated internal regulation and simplified the genetics by removing or recoding genes, scrambling gene order and replacing all non-coding DNA with synthetic genetic parts. This process results in a 16 kb cluster that shares no sequence identity, regulation or organizational principles with SPI-1. Building this simplified system led to the discovery of essential roles for an internal start site (SpaO) and small RNA (InvR). Further, it can be controlled using synthetic regulatory circuits, including under SPI-1 repressing conditions. This work reveals an incredible post-transcriptional robustness in T3SS assembly and aids its control as a tool in biotechnology. Nature Publishing Group 2017-05-09 /pmc/articles/PMC5436071/ /pubmed/28485369 http://dx.doi.org/10.1038/ncomms14737 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Song, Miryoung Sukovich, David J. Ciccarelli, Luciano Mayr, Julia Fernandez-Rodriguez, Jesus Mirsky, Ethan A. Tucker, Alex C. Gordon, D. Benjamin Marlovits, Thomas C. Voigt, Christopher A. Control of type III protein secretion using a minimal genetic system |
title | Control of type III protein secretion using a minimal genetic system |
title_full | Control of type III protein secretion using a minimal genetic system |
title_fullStr | Control of type III protein secretion using a minimal genetic system |
title_full_unstemmed | Control of type III protein secretion using a minimal genetic system |
title_short | Control of type III protein secretion using a minimal genetic system |
title_sort | control of type iii protein secretion using a minimal genetic system |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5436071/ https://www.ncbi.nlm.nih.gov/pubmed/28485369 http://dx.doi.org/10.1038/ncomms14737 |
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