Cargando…

Impedimetric Analysis of the Effect of Decellularized Porcine Heart Scaffold on Human Fibrosarcoma, Endothelial, and Cardiomyocyte Cell Lines

BACKGROUND: Experiments on porcine heart scaffold represent significant assays in development of immunoneutral materials for cardiac surgery. Characterization of cell-cell and cell-scaffold interactions is essential to understand the homing process of cardiac cells into the scaffolds. MATERIAL/METHO...

Descripción completa

Detalles Bibliográficos
Autores principales: Bäcker, Henrik, Polgár, Livia, Soós, Pal, Lajkó, Eszter, Láng, Orsolya, Merkely, Bela, Szabó, Gabor, Dohmen, Pascal M., Weymann, Alexander, Kőhidai, Laszlo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Scientific Literature, Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5436501/
https://www.ncbi.nlm.nih.gov/pubmed/28493851
http://dx.doi.org/10.12659/MSM.901527
_version_ 1783237417789030400
author Bäcker, Henrik
Polgár, Livia
Soós, Pal
Lajkó, Eszter
Láng, Orsolya
Merkely, Bela
Szabó, Gabor
Dohmen, Pascal M.
Weymann, Alexander
Kőhidai, Laszlo
author_facet Bäcker, Henrik
Polgár, Livia
Soós, Pal
Lajkó, Eszter
Láng, Orsolya
Merkely, Bela
Szabó, Gabor
Dohmen, Pascal M.
Weymann, Alexander
Kőhidai, Laszlo
author_sort Bäcker, Henrik
collection PubMed
description BACKGROUND: Experiments on porcine heart scaffold represent significant assays in development of immunoneutral materials for cardiac surgery. Characterization of cell-cell and cell-scaffold interactions is essential to understand the homing process of cardiac cells into the scaffolds. MATERIAL/METHODS: In the present study, the highly sensitive and real-time impedimetric technique of xCELLigence SP was used to monitor cell adhesion, which is the key process of recellularization in heart scaffolds. Our objectives were: (i) to characterize the effect of decellularized porcine heart scaffold on cell adhesion of human cardiovascular cells potentially used in the recellularization process; and (ii) to investigate cell-extracellular matrix element interactions for building artificial multi-layer systems, applied as cellular models of recellularization experiments. Human fibrosarcoma, endothelial, and cardiomyocyte cells were investigated and the effect of decellularized porcine heart scaffold (HS) and fibronectin on cell adhesion was examined. Adhesion was quantified as slope of curves. RESULTS: Heart scaffold had neutral effect on cardiomyocytes as well as on endothelial cells. Adhesion of cardiomyocytes was increased by fibronectin (1.480±0.021) compared to control (0.745±0.029). The combination of fibronectin and HS induced stronger adhesion of cardiomyocytes (2.407±0.634) than fibronectin alone. Endothelial and fibrosarcoma cells showed similarly strong adhesion profiles with marked enhancer effect by fibronectin. CONCLUSIONS: Decellularized porcine HS does not inhibit adhesion of human cardiovascular cells at the cell biological level, while fibronectin has strong cell adhesion-inducer effect, as well as an enhancer effect on activity of HS. Consequently, decellularized porcine hearts could be used as scaffolds for recellularization with cardiomyocytes and endothelial cells with fibronectin acting as a regulator, leading to construction of working bioartificial hearts.
format Online
Article
Text
id pubmed-5436501
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher International Scientific Literature, Inc.
record_format MEDLINE/PubMed
spelling pubmed-54365012017-05-30 Impedimetric Analysis of the Effect of Decellularized Porcine Heart Scaffold on Human Fibrosarcoma, Endothelial, and Cardiomyocyte Cell Lines Bäcker, Henrik Polgár, Livia Soós, Pal Lajkó, Eszter Láng, Orsolya Merkely, Bela Szabó, Gabor Dohmen, Pascal M. Weymann, Alexander Kőhidai, Laszlo Med Sci Monit Animal Study BACKGROUND: Experiments on porcine heart scaffold represent significant assays in development of immunoneutral materials for cardiac surgery. Characterization of cell-cell and cell-scaffold interactions is essential to understand the homing process of cardiac cells into the scaffolds. MATERIAL/METHODS: In the present study, the highly sensitive and real-time impedimetric technique of xCELLigence SP was used to monitor cell adhesion, which is the key process of recellularization in heart scaffolds. Our objectives were: (i) to characterize the effect of decellularized porcine heart scaffold on cell adhesion of human cardiovascular cells potentially used in the recellularization process; and (ii) to investigate cell-extracellular matrix element interactions for building artificial multi-layer systems, applied as cellular models of recellularization experiments. Human fibrosarcoma, endothelial, and cardiomyocyte cells were investigated and the effect of decellularized porcine heart scaffold (HS) and fibronectin on cell adhesion was examined. Adhesion was quantified as slope of curves. RESULTS: Heart scaffold had neutral effect on cardiomyocytes as well as on endothelial cells. Adhesion of cardiomyocytes was increased by fibronectin (1.480±0.021) compared to control (0.745±0.029). The combination of fibronectin and HS induced stronger adhesion of cardiomyocytes (2.407±0.634) than fibronectin alone. Endothelial and fibrosarcoma cells showed similarly strong adhesion profiles with marked enhancer effect by fibronectin. CONCLUSIONS: Decellularized porcine HS does not inhibit adhesion of human cardiovascular cells at the cell biological level, while fibronectin has strong cell adhesion-inducer effect, as well as an enhancer effect on activity of HS. Consequently, decellularized porcine hearts could be used as scaffolds for recellularization with cardiomyocytes and endothelial cells with fibronectin acting as a regulator, leading to construction of working bioartificial hearts. International Scientific Literature, Inc. 2017-05-11 /pmc/articles/PMC5436501/ /pubmed/28493851 http://dx.doi.org/10.12659/MSM.901527 Text en © Med Sci Monit, 2017 This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) )
spellingShingle Animal Study
Bäcker, Henrik
Polgár, Livia
Soós, Pal
Lajkó, Eszter
Láng, Orsolya
Merkely, Bela
Szabó, Gabor
Dohmen, Pascal M.
Weymann, Alexander
Kőhidai, Laszlo
Impedimetric Analysis of the Effect of Decellularized Porcine Heart Scaffold on Human Fibrosarcoma, Endothelial, and Cardiomyocyte Cell Lines
title Impedimetric Analysis of the Effect of Decellularized Porcine Heart Scaffold on Human Fibrosarcoma, Endothelial, and Cardiomyocyte Cell Lines
title_full Impedimetric Analysis of the Effect of Decellularized Porcine Heart Scaffold on Human Fibrosarcoma, Endothelial, and Cardiomyocyte Cell Lines
title_fullStr Impedimetric Analysis of the Effect of Decellularized Porcine Heart Scaffold on Human Fibrosarcoma, Endothelial, and Cardiomyocyte Cell Lines
title_full_unstemmed Impedimetric Analysis of the Effect of Decellularized Porcine Heart Scaffold on Human Fibrosarcoma, Endothelial, and Cardiomyocyte Cell Lines
title_short Impedimetric Analysis of the Effect of Decellularized Porcine Heart Scaffold on Human Fibrosarcoma, Endothelial, and Cardiomyocyte Cell Lines
title_sort impedimetric analysis of the effect of decellularized porcine heart scaffold on human fibrosarcoma, endothelial, and cardiomyocyte cell lines
topic Animal Study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5436501/
https://www.ncbi.nlm.nih.gov/pubmed/28493851
http://dx.doi.org/10.12659/MSM.901527
work_keys_str_mv AT backerhenrik impedimetricanalysisoftheeffectofdecellularizedporcineheartscaffoldonhumanfibrosarcomaendothelialandcardiomyocytecelllines
AT polgarlivia impedimetricanalysisoftheeffectofdecellularizedporcineheartscaffoldonhumanfibrosarcomaendothelialandcardiomyocytecelllines
AT soospal impedimetricanalysisoftheeffectofdecellularizedporcineheartscaffoldonhumanfibrosarcomaendothelialandcardiomyocytecelllines
AT lajkoeszter impedimetricanalysisoftheeffectofdecellularizedporcineheartscaffoldonhumanfibrosarcomaendothelialandcardiomyocytecelllines
AT langorsolya impedimetricanalysisoftheeffectofdecellularizedporcineheartscaffoldonhumanfibrosarcomaendothelialandcardiomyocytecelllines
AT merkelybela impedimetricanalysisoftheeffectofdecellularizedporcineheartscaffoldonhumanfibrosarcomaendothelialandcardiomyocytecelllines
AT szabogabor impedimetricanalysisoftheeffectofdecellularizedporcineheartscaffoldonhumanfibrosarcomaendothelialandcardiomyocytecelllines
AT dohmenpascalm impedimetricanalysisoftheeffectofdecellularizedporcineheartscaffoldonhumanfibrosarcomaendothelialandcardiomyocytecelllines
AT weymannalexander impedimetricanalysisoftheeffectofdecellularizedporcineheartscaffoldonhumanfibrosarcomaendothelialandcardiomyocytecelllines
AT kohidailaszlo impedimetricanalysisoftheeffectofdecellularizedporcineheartscaffoldonhumanfibrosarcomaendothelialandcardiomyocytecelllines