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Methyltransferase-directed covalent coupling of fluorophores to DNA

We report an assay for determining the number of fluorophores conjugated to single plasmid DNA molecules and apply this to compare the efficiency of fluorophore coupling strategies for covalent DNA labelling. We compare a copper-catalyzed azide–alkyne cycloaddition reaction, amine to N-hydroxysuccin...

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Detalles Bibliográficos
Autores principales:	Lauer, Milena Helmer, Vranken, Charlotte, Deen, Jochem, Frederickx, Wout, Vanderlinden, Willem, Wand, Nathaniel, Leen, Volker, Gehlen, Marcelo H., Hofkens, Johan, Neely, Robert K.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Royal Society of Chemistry 2017
Materias:	Chemistry
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5436541/ https://www.ncbi.nlm.nih.gov/pubmed/28580113 http://dx.doi.org/10.1039/c6sc04229e

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