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Evaluation of the Sepsis Flow Chip assay for the diagnosis of blood infections
BACKGROUND: Blood infections are serious complex conditions that generally require rapid diagnosis and treatment. The big challenge is to reduce the time necessary to make a diagnosis with current clinical microbiological methods so as to improve the treatment given to patients. METHODS: In this stu...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5436663/ https://www.ncbi.nlm.nih.gov/pubmed/28542614 http://dx.doi.org/10.1371/journal.pone.0177627 |
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author | Galiana, Antonio Coy, Javier Gimeno, Adelina Guzman, Noemi Marco Rosales, Francisco Merino, Esperanza Royo, Gloria Rodríguez, Juan Carlos |
author_facet | Galiana, Antonio Coy, Javier Gimeno, Adelina Guzman, Noemi Marco Rosales, Francisco Merino, Esperanza Royo, Gloria Rodríguez, Juan Carlos |
author_sort | Galiana, Antonio |
collection | PubMed |
description | BACKGROUND: Blood infections are serious complex conditions that generally require rapid diagnosis and treatment. The big challenge is to reduce the time necessary to make a diagnosis with current clinical microbiological methods so as to improve the treatment given to patients. METHODS: In this study, we assess for the first time the Sepsis Flow Chip assay, which is a novel diagnostic assay for simultaneous rapid-detection of the vast majority of bloodstream pathogens, including Gram-positive and Gram-negative bacteria and fungi, in the same assay, and for the detection of most common antibiotic resistance genes. The SFC assay is based on multiplex PCR and low density DNA arrays. RESULTS: Positive blood cultures from 202 consecutive bacteremia patients were analyzed by SFC assay and the results were compared with the results obtained by the gold standard methodology used in clinical microbiology diagnostic laboratories (EUCAST guidelines). SFC assay overall sensitivity and specificity for bacterial identification were 93.3% and 100% respectively and sensitivity and specificity for the identification of antibiotic genetic resistance determinants were 93.6% and 100% respectively. CONCLUSIONS: This is the first evaluation of SFC assay in clinical samples. This new method appears to be very promising by combining the high number of distinct pathogens and genetic resistance determinants identified in a single assay. Further investigations should be done to evaluate the usefulness of this assay in combination with clinical multidisciplinary groups (stewardship), in order for the results to be applied appropriately to the management of patients`infectious processes. |
format | Online Article Text |
id | pubmed-5436663 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-54366632017-05-27 Evaluation of the Sepsis Flow Chip assay for the diagnosis of blood infections Galiana, Antonio Coy, Javier Gimeno, Adelina Guzman, Noemi Marco Rosales, Francisco Merino, Esperanza Royo, Gloria Rodríguez, Juan Carlos PLoS One Research Article BACKGROUND: Blood infections are serious complex conditions that generally require rapid diagnosis and treatment. The big challenge is to reduce the time necessary to make a diagnosis with current clinical microbiological methods so as to improve the treatment given to patients. METHODS: In this study, we assess for the first time the Sepsis Flow Chip assay, which is a novel diagnostic assay for simultaneous rapid-detection of the vast majority of bloodstream pathogens, including Gram-positive and Gram-negative bacteria and fungi, in the same assay, and for the detection of most common antibiotic resistance genes. The SFC assay is based on multiplex PCR and low density DNA arrays. RESULTS: Positive blood cultures from 202 consecutive bacteremia patients were analyzed by SFC assay and the results were compared with the results obtained by the gold standard methodology used in clinical microbiology diagnostic laboratories (EUCAST guidelines). SFC assay overall sensitivity and specificity for bacterial identification were 93.3% and 100% respectively and sensitivity and specificity for the identification of antibiotic genetic resistance determinants were 93.6% and 100% respectively. CONCLUSIONS: This is the first evaluation of SFC assay in clinical samples. This new method appears to be very promising by combining the high number of distinct pathogens and genetic resistance determinants identified in a single assay. Further investigations should be done to evaluate the usefulness of this assay in combination with clinical multidisciplinary groups (stewardship), in order for the results to be applied appropriately to the management of patients`infectious processes. Public Library of Science 2017-05-18 /pmc/articles/PMC5436663/ /pubmed/28542614 http://dx.doi.org/10.1371/journal.pone.0177627 Text en © 2017 Galiana et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Galiana, Antonio Coy, Javier Gimeno, Adelina Guzman, Noemi Marco Rosales, Francisco Merino, Esperanza Royo, Gloria Rodríguez, Juan Carlos Evaluation of the Sepsis Flow Chip assay for the diagnosis of blood infections |
title | Evaluation of the Sepsis Flow Chip assay for the diagnosis of blood infections |
title_full | Evaluation of the Sepsis Flow Chip assay for the diagnosis of blood infections |
title_fullStr | Evaluation of the Sepsis Flow Chip assay for the diagnosis of blood infections |
title_full_unstemmed | Evaluation of the Sepsis Flow Chip assay for the diagnosis of blood infections |
title_short | Evaluation of the Sepsis Flow Chip assay for the diagnosis of blood infections |
title_sort | evaluation of the sepsis flow chip assay for the diagnosis of blood infections |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5436663/ https://www.ncbi.nlm.nih.gov/pubmed/28542614 http://dx.doi.org/10.1371/journal.pone.0177627 |
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