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Human plasma metabolomics in age-related macular degeneration (AMD) using nuclear magnetic resonance spectroscopy

PURPOSE: To differentiate the plasma metabolomic profile of patients with age related macular degeneration (AMD) from that of controls, by Nuclear Magnetic Resonance (NMR) spectroscopy. METHODS: Two cohorts (total of 396 subjects) representative of central Portugal and Boston, USA phenotypes were st...

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Autores principales: Laíns, Inês, Duarte, Daniela, Barros, António S., Martins, Ana Sofia, Gil, João, Miller, John B., Marques, Marco, Mesquita, Tânia, Kim, Ivana K., Cachulo, Maria da Luz, Vavvas, Demetrios, Carreira, Isabel M., Murta, Joaquim N., Silva, Rufino, Miller, Joan W., Husain, Deeba, Gil, Ana M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5436712/
https://www.ncbi.nlm.nih.gov/pubmed/28542375
http://dx.doi.org/10.1371/journal.pone.0177749
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author Laíns, Inês
Duarte, Daniela
Barros, António S.
Martins, Ana Sofia
Gil, João
Miller, John B.
Marques, Marco
Mesquita, Tânia
Kim, Ivana K.
Cachulo, Maria da Luz
Vavvas, Demetrios
Carreira, Isabel M.
Murta, Joaquim N.
Silva, Rufino
Miller, Joan W.
Husain, Deeba
Gil, Ana M.
author_facet Laíns, Inês
Duarte, Daniela
Barros, António S.
Martins, Ana Sofia
Gil, João
Miller, John B.
Marques, Marco
Mesquita, Tânia
Kim, Ivana K.
Cachulo, Maria da Luz
Vavvas, Demetrios
Carreira, Isabel M.
Murta, Joaquim N.
Silva, Rufino
Miller, Joan W.
Husain, Deeba
Gil, Ana M.
author_sort Laíns, Inês
collection PubMed
description PURPOSE: To differentiate the plasma metabolomic profile of patients with age related macular degeneration (AMD) from that of controls, by Nuclear Magnetic Resonance (NMR) spectroscopy. METHODS: Two cohorts (total of 396 subjects) representative of central Portugal and Boston, USA phenotypes were studied. For each cohort, subjects were grouped according to AMD stage (early, intermediate and late). Multivariate analysis of plasma NMR spectra was performed, followed by signal integration and univariate analysis. RESULTS: Small changes were detected in the levels of some amino acids, organic acids, dimethyl sulfone and specific lipid moieties, thus providing some biochemical information on the disease. The possible confounding effects of gender, smoking history and age were assessed in each cohort and found to be minimal when compared to that of the disease. A similar observation was noted in relation to age-related comorbidities. Furthermore, partially distinct putative AMD metabolite fingerprints were noted for the two cohorts studied, reflecting the importance of nutritional and other lifestyle habits in determining AMD metabolic response and potential biomarker fingerprints. Notably, some of the metabolite changes detected were noted as potentially differentiating controls from patients diagnosed with early AMD. CONCLUSION: For the first time, this study showed metabolite changes in the plasma of patients with AMD as compared to controls, using NMR. Geographical origins were seen to affect AMD patients´ metabolic profile and some metabolites were found to be valuable in potentially differentiating controls from early stage AMD patients. Metabolomics has the potential of identifying biomarkers for AMD, and further work in this area is warranted.
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spelling pubmed-54367122017-05-27 Human plasma metabolomics in age-related macular degeneration (AMD) using nuclear magnetic resonance spectroscopy Laíns, Inês Duarte, Daniela Barros, António S. Martins, Ana Sofia Gil, João Miller, John B. Marques, Marco Mesquita, Tânia Kim, Ivana K. Cachulo, Maria da Luz Vavvas, Demetrios Carreira, Isabel M. Murta, Joaquim N. Silva, Rufino Miller, Joan W. Husain, Deeba Gil, Ana M. PLoS One Research Article PURPOSE: To differentiate the plasma metabolomic profile of patients with age related macular degeneration (AMD) from that of controls, by Nuclear Magnetic Resonance (NMR) spectroscopy. METHODS: Two cohorts (total of 396 subjects) representative of central Portugal and Boston, USA phenotypes were studied. For each cohort, subjects were grouped according to AMD stage (early, intermediate and late). Multivariate analysis of plasma NMR spectra was performed, followed by signal integration and univariate analysis. RESULTS: Small changes were detected in the levels of some amino acids, organic acids, dimethyl sulfone and specific lipid moieties, thus providing some biochemical information on the disease. The possible confounding effects of gender, smoking history and age were assessed in each cohort and found to be minimal when compared to that of the disease. A similar observation was noted in relation to age-related comorbidities. Furthermore, partially distinct putative AMD metabolite fingerprints were noted for the two cohorts studied, reflecting the importance of nutritional and other lifestyle habits in determining AMD metabolic response and potential biomarker fingerprints. Notably, some of the metabolite changes detected were noted as potentially differentiating controls from patients diagnosed with early AMD. CONCLUSION: For the first time, this study showed metabolite changes in the plasma of patients with AMD as compared to controls, using NMR. Geographical origins were seen to affect AMD patients´ metabolic profile and some metabolites were found to be valuable in potentially differentiating controls from early stage AMD patients. Metabolomics has the potential of identifying biomarkers for AMD, and further work in this area is warranted. Public Library of Science 2017-05-18 /pmc/articles/PMC5436712/ /pubmed/28542375 http://dx.doi.org/10.1371/journal.pone.0177749 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
spellingShingle Research Article
Laíns, Inês
Duarte, Daniela
Barros, António S.
Martins, Ana Sofia
Gil, João
Miller, John B.
Marques, Marco
Mesquita, Tânia
Kim, Ivana K.
Cachulo, Maria da Luz
Vavvas, Demetrios
Carreira, Isabel M.
Murta, Joaquim N.
Silva, Rufino
Miller, Joan W.
Husain, Deeba
Gil, Ana M.
Human plasma metabolomics in age-related macular degeneration (AMD) using nuclear magnetic resonance spectroscopy
title Human plasma metabolomics in age-related macular degeneration (AMD) using nuclear magnetic resonance spectroscopy
title_full Human plasma metabolomics in age-related macular degeneration (AMD) using nuclear magnetic resonance spectroscopy
title_fullStr Human plasma metabolomics in age-related macular degeneration (AMD) using nuclear magnetic resonance spectroscopy
title_full_unstemmed Human plasma metabolomics in age-related macular degeneration (AMD) using nuclear magnetic resonance spectroscopy
title_short Human plasma metabolomics in age-related macular degeneration (AMD) using nuclear magnetic resonance spectroscopy
title_sort human plasma metabolomics in age-related macular degeneration (amd) using nuclear magnetic resonance spectroscopy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5436712/
https://www.ncbi.nlm.nih.gov/pubmed/28542375
http://dx.doi.org/10.1371/journal.pone.0177749
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