Cargando…

Proteome remodelling by the stress sigma factor RpoS/σ(S) in Salmonella: identification of small proteins and evidence for post-transcriptional regulation

The RpoS/σ(S) sigma subunit of RNA polymerase is the master regulator of the general stress response in many Gram-negative bacteria. Extensive studies have been conducted on σ(S)-regulated gene expression at the transcriptional level. In contrast, very limited information regarding the impact of σ(S...

Descripción completa

Detalles Bibliográficos
Autores principales: Lago, Magali, Monteil, Véronique, Douche, Thibaut, Guglielmini, Julien, Criscuolo, Alexis, Maufrais, Corinne, Matondo, Mariette, Norel, Françoise
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5437024/
https://www.ncbi.nlm.nih.gov/pubmed/28522802
http://dx.doi.org/10.1038/s41598-017-02362-3
_version_ 1783237506413625344
author Lago, Magali
Monteil, Véronique
Douche, Thibaut
Guglielmini, Julien
Criscuolo, Alexis
Maufrais, Corinne
Matondo, Mariette
Norel, Françoise
author_facet Lago, Magali
Monteil, Véronique
Douche, Thibaut
Guglielmini, Julien
Criscuolo, Alexis
Maufrais, Corinne
Matondo, Mariette
Norel, Françoise
author_sort Lago, Magali
collection PubMed
description The RpoS/σ(S) sigma subunit of RNA polymerase is the master regulator of the general stress response in many Gram-negative bacteria. Extensive studies have been conducted on σ(S)-regulated gene expression at the transcriptional level. In contrast, very limited information regarding the impact of σ(S) on global protein production is available. In this study, we used a mass spectrometry-based proteomics approach to explore the wide σ(S)-dependent proteome of the human pathogen Salmonella enterica serovar Typhimurium. Our present goals were twofold: (1) to survey the protein changes associated with the ΔrpoS mutation and (2) to assess the coding capacity of σ(S)-dependent small RNAs. Our proteomics data, and complementary assays, unravelled the large impact of σ(S) on the Salmonella proteome, and validated expression and σ(S) regulation of twenty uncharacterized small proteins of 27 to 96 amino acids. Furthermore, a large number of genes regulated at the protein level only were identified, suggesting that post-transcriptional regulation is an important component of the σ(S) response. Novel aspects of σ(S) in the control of important catabolic pathways such as myo-inositol, L-fucose, propanediol, and ethanolamine were illuminated by this work, providing new insights into the physiological remodelling involved in bacterial adaptation to a non-actively growing state.
format Online
Article
Text
id pubmed-5437024
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-54370242017-05-19 Proteome remodelling by the stress sigma factor RpoS/σ(S) in Salmonella: identification of small proteins and evidence for post-transcriptional regulation Lago, Magali Monteil, Véronique Douche, Thibaut Guglielmini, Julien Criscuolo, Alexis Maufrais, Corinne Matondo, Mariette Norel, Françoise Sci Rep Article The RpoS/σ(S) sigma subunit of RNA polymerase is the master regulator of the general stress response in many Gram-negative bacteria. Extensive studies have been conducted on σ(S)-regulated gene expression at the transcriptional level. In contrast, very limited information regarding the impact of σ(S) on global protein production is available. In this study, we used a mass spectrometry-based proteomics approach to explore the wide σ(S)-dependent proteome of the human pathogen Salmonella enterica serovar Typhimurium. Our present goals were twofold: (1) to survey the protein changes associated with the ΔrpoS mutation and (2) to assess the coding capacity of σ(S)-dependent small RNAs. Our proteomics data, and complementary assays, unravelled the large impact of σ(S) on the Salmonella proteome, and validated expression and σ(S) regulation of twenty uncharacterized small proteins of 27 to 96 amino acids. Furthermore, a large number of genes regulated at the protein level only were identified, suggesting that post-transcriptional regulation is an important component of the σ(S) response. Novel aspects of σ(S) in the control of important catabolic pathways such as myo-inositol, L-fucose, propanediol, and ethanolamine were illuminated by this work, providing new insights into the physiological remodelling involved in bacterial adaptation to a non-actively growing state. Nature Publishing Group UK 2017-05-18 /pmc/articles/PMC5437024/ /pubmed/28522802 http://dx.doi.org/10.1038/s41598-017-02362-3 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Lago, Magali
Monteil, Véronique
Douche, Thibaut
Guglielmini, Julien
Criscuolo, Alexis
Maufrais, Corinne
Matondo, Mariette
Norel, Françoise
Proteome remodelling by the stress sigma factor RpoS/σ(S) in Salmonella: identification of small proteins and evidence for post-transcriptional regulation
title Proteome remodelling by the stress sigma factor RpoS/σ(S) in Salmonella: identification of small proteins and evidence for post-transcriptional regulation
title_full Proteome remodelling by the stress sigma factor RpoS/σ(S) in Salmonella: identification of small proteins and evidence for post-transcriptional regulation
title_fullStr Proteome remodelling by the stress sigma factor RpoS/σ(S) in Salmonella: identification of small proteins and evidence for post-transcriptional regulation
title_full_unstemmed Proteome remodelling by the stress sigma factor RpoS/σ(S) in Salmonella: identification of small proteins and evidence for post-transcriptional regulation
title_short Proteome remodelling by the stress sigma factor RpoS/σ(S) in Salmonella: identification of small proteins and evidence for post-transcriptional regulation
title_sort proteome remodelling by the stress sigma factor rpos/σ(s) in salmonella: identification of small proteins and evidence for post-transcriptional regulation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5437024/
https://www.ncbi.nlm.nih.gov/pubmed/28522802
http://dx.doi.org/10.1038/s41598-017-02362-3
work_keys_str_mv AT lagomagali proteomeremodellingbythestresssigmafactorrposssinsalmonellaidentificationofsmallproteinsandevidenceforposttranscriptionalregulation
AT monteilveronique proteomeremodellingbythestresssigmafactorrposssinsalmonellaidentificationofsmallproteinsandevidenceforposttranscriptionalregulation
AT douchethibaut proteomeremodellingbythestresssigmafactorrposssinsalmonellaidentificationofsmallproteinsandevidenceforposttranscriptionalregulation
AT guglielminijulien proteomeremodellingbythestresssigmafactorrposssinsalmonellaidentificationofsmallproteinsandevidenceforposttranscriptionalregulation
AT criscuoloalexis proteomeremodellingbythestresssigmafactorrposssinsalmonellaidentificationofsmallproteinsandevidenceforposttranscriptionalregulation
AT maufraiscorinne proteomeremodellingbythestresssigmafactorrposssinsalmonellaidentificationofsmallproteinsandevidenceforposttranscriptionalregulation
AT matondomariette proteomeremodellingbythestresssigmafactorrposssinsalmonellaidentificationofsmallproteinsandevidenceforposttranscriptionalregulation
AT norelfrancoise proteomeremodellingbythestresssigmafactorrposssinsalmonellaidentificationofsmallproteinsandevidenceforposttranscriptionalregulation