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Efficient modification of λ-DNA substrates for single-molecule studies

Single-molecule studies of protein-nucleic acid interactions frequently require site-specific modification of long DNA substrates. The bacteriophage λ is a convenient source of high quality long (48.5 kb) DNA. However, introducing specific sequences, tertiary structures, and chemical modifications i...

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Detalles Bibliográficos
Autores principales:	Kim, Yoori, de la Torre, Armando, Leal, Andrew A., Finkelstein, Ilya J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2017
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5437064/ https://www.ncbi.nlm.nih.gov/pubmed/28522818 http://dx.doi.org/10.1038/s41598-017-01984-x

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