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Plasmonics Enhanced Smartphone Fluorescence Microscopy
Smartphone fluorescence microscopy has various applications in point-of-care (POC) testing and diagnostics, ranging from e.g., quantification of immunoassays, detection of microorganisms, to sensing of viruses. An important need in smartphone-based microscopy and sensing techniques is to improve the...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5437072/ https://www.ncbi.nlm.nih.gov/pubmed/28522808 http://dx.doi.org/10.1038/s41598-017-02395-8 |
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author | Wei, Qingshan Acuna, Guillermo Kim, Seungkyeum Vietz, Carolin Tseng, Derek Chae, Jongjae Shir, Daniel Luo, Wei Tinnefeld, Philip Ozcan, Aydogan |
author_facet | Wei, Qingshan Acuna, Guillermo Kim, Seungkyeum Vietz, Carolin Tseng, Derek Chae, Jongjae Shir, Daniel Luo, Wei Tinnefeld, Philip Ozcan, Aydogan |
author_sort | Wei, Qingshan |
collection | PubMed |
description | Smartphone fluorescence microscopy has various applications in point-of-care (POC) testing and diagnostics, ranging from e.g., quantification of immunoassays, detection of microorganisms, to sensing of viruses. An important need in smartphone-based microscopy and sensing techniques is to improve the detection sensitivity to enable quantification of extremely low concentrations of target molecules. Here, we demonstrate a general strategy to enhance the detection sensitivity of a smartphone-based fluorescence microscope by using surface-enhanced fluorescence (SEF) created by a thin metal-film. In this plasmonic design, the samples are placed on a silver-coated glass slide with a thin spacer, and excited by a laser-diode from the backside through a glass hemisphere, generating surface plasmon polaritons. We optimized this mobile SEF system by tuning the metal-film thickness, spacer distance, excitation angle and polarization, and achieved ~10-fold enhancement in fluorescence intensity compared to a bare glass substrate, which enabled us to image single fluorescent particles as small as 50 nm in diameter and single quantum-dots. Furthermore, we quantified the detection limit of this platform by using DNA origami-based brightness standards, demonstrating that ~80 fluorophores per diffraction-limited spot can be readily detected by our mobile microscope, which opens up new opportunities for POC diagnostics and sensing applications in resource-limited-settings. |
format | Online Article Text |
id | pubmed-5437072 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-54370722017-05-19 Plasmonics Enhanced Smartphone Fluorescence Microscopy Wei, Qingshan Acuna, Guillermo Kim, Seungkyeum Vietz, Carolin Tseng, Derek Chae, Jongjae Shir, Daniel Luo, Wei Tinnefeld, Philip Ozcan, Aydogan Sci Rep Article Smartphone fluorescence microscopy has various applications in point-of-care (POC) testing and diagnostics, ranging from e.g., quantification of immunoassays, detection of microorganisms, to sensing of viruses. An important need in smartphone-based microscopy and sensing techniques is to improve the detection sensitivity to enable quantification of extremely low concentrations of target molecules. Here, we demonstrate a general strategy to enhance the detection sensitivity of a smartphone-based fluorescence microscope by using surface-enhanced fluorescence (SEF) created by a thin metal-film. In this plasmonic design, the samples are placed on a silver-coated glass slide with a thin spacer, and excited by a laser-diode from the backside through a glass hemisphere, generating surface plasmon polaritons. We optimized this mobile SEF system by tuning the metal-film thickness, spacer distance, excitation angle and polarization, and achieved ~10-fold enhancement in fluorescence intensity compared to a bare glass substrate, which enabled us to image single fluorescent particles as small as 50 nm in diameter and single quantum-dots. Furthermore, we quantified the detection limit of this platform by using DNA origami-based brightness standards, demonstrating that ~80 fluorophores per diffraction-limited spot can be readily detected by our mobile microscope, which opens up new opportunities for POC diagnostics and sensing applications in resource-limited-settings. Nature Publishing Group UK 2017-05-18 /pmc/articles/PMC5437072/ /pubmed/28522808 http://dx.doi.org/10.1038/s41598-017-02395-8 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Wei, Qingshan Acuna, Guillermo Kim, Seungkyeum Vietz, Carolin Tseng, Derek Chae, Jongjae Shir, Daniel Luo, Wei Tinnefeld, Philip Ozcan, Aydogan Plasmonics Enhanced Smartphone Fluorescence Microscopy |
title | Plasmonics Enhanced Smartphone Fluorescence Microscopy |
title_full | Plasmonics Enhanced Smartphone Fluorescence Microscopy |
title_fullStr | Plasmonics Enhanced Smartphone Fluorescence Microscopy |
title_full_unstemmed | Plasmonics Enhanced Smartphone Fluorescence Microscopy |
title_short | Plasmonics Enhanced Smartphone Fluorescence Microscopy |
title_sort | plasmonics enhanced smartphone fluorescence microscopy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5437072/ https://www.ncbi.nlm.nih.gov/pubmed/28522808 http://dx.doi.org/10.1038/s41598-017-02395-8 |
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