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Kup-mediated Cs(+) uptake and Kdp-driven K(+) uptake coordinate to promote cell growth during excess Cs(+) conditions in Escherichia coli

The physiological effects of caesium (Cs) on living cells are poorly understood. Here, we examined the physiological role of Cs(+) on the activity of the potassium transporters in E. coli. In the absence of potassium (K(+)), Kup-mediated Cs(+) uptake partially supported cell growth, however, at a mu...

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Autores principales: Tanudjaja, Ellen, Hoshi, Naomi, Su, Yi-Hsin, Hamamoto, Shin, Uozumi, Nobuyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5437092/
https://www.ncbi.nlm.nih.gov/pubmed/28522840
http://dx.doi.org/10.1038/s41598-017-02164-7
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author Tanudjaja, Ellen
Hoshi, Naomi
Su, Yi-Hsin
Hamamoto, Shin
Uozumi, Nobuyuki
author_facet Tanudjaja, Ellen
Hoshi, Naomi
Su, Yi-Hsin
Hamamoto, Shin
Uozumi, Nobuyuki
author_sort Tanudjaja, Ellen
collection PubMed
description The physiological effects of caesium (Cs) on living cells are poorly understood. Here, we examined the physiological role of Cs(+) on the activity of the potassium transporters in E. coli. In the absence of potassium (K(+)), Kup-mediated Cs(+) uptake partially supported cell growth, however, at a much lower rate than with sufficient K(+). In K(+)-limited medium (0.1 mM), the presence of Cs(+) (up to 25 mM) in the medium enhanced growth as much as control medium containing 1 mM K(+). This effect depended on the maintenance of basal levels of intracellular K(+) by other K(+) uptake transporters. Higher amounts of K(+) (1 mM) in the medium eliminated the positive effect of Cs(+) on growth, and revealed the inhibitory effect of high Cs(+) on the growth of wild-type E. coli. Cells lacking Kdp, TrkG and TrkH but expressing Kup grew less well when Cs(+) was increased in the medium. A kdp mutant contained an increased ratio of Cs(+)/K(+) in the presence of high Cs(+) in the medium and consequently was strongly inhibited in growth. Taken together, under excess Cs(+) conditions Kup-mediated Cs(+) influx sustains cell growth, which is supported by intracellular K(+) supplied by Kdp.
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spelling pubmed-54370922017-05-19 Kup-mediated Cs(+) uptake and Kdp-driven K(+) uptake coordinate to promote cell growth during excess Cs(+) conditions in Escherichia coli Tanudjaja, Ellen Hoshi, Naomi Su, Yi-Hsin Hamamoto, Shin Uozumi, Nobuyuki Sci Rep Article The physiological effects of caesium (Cs) on living cells are poorly understood. Here, we examined the physiological role of Cs(+) on the activity of the potassium transporters in E. coli. In the absence of potassium (K(+)), Kup-mediated Cs(+) uptake partially supported cell growth, however, at a much lower rate than with sufficient K(+). In K(+)-limited medium (0.1 mM), the presence of Cs(+) (up to 25 mM) in the medium enhanced growth as much as control medium containing 1 mM K(+). This effect depended on the maintenance of basal levels of intracellular K(+) by other K(+) uptake transporters. Higher amounts of K(+) (1 mM) in the medium eliminated the positive effect of Cs(+) on growth, and revealed the inhibitory effect of high Cs(+) on the growth of wild-type E. coli. Cells lacking Kdp, TrkG and TrkH but expressing Kup grew less well when Cs(+) was increased in the medium. A kdp mutant contained an increased ratio of Cs(+)/K(+) in the presence of high Cs(+) in the medium and consequently was strongly inhibited in growth. Taken together, under excess Cs(+) conditions Kup-mediated Cs(+) influx sustains cell growth, which is supported by intracellular K(+) supplied by Kdp. Nature Publishing Group UK 2017-05-18 /pmc/articles/PMC5437092/ /pubmed/28522840 http://dx.doi.org/10.1038/s41598-017-02164-7 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Tanudjaja, Ellen
Hoshi, Naomi
Su, Yi-Hsin
Hamamoto, Shin
Uozumi, Nobuyuki
Kup-mediated Cs(+) uptake and Kdp-driven K(+) uptake coordinate to promote cell growth during excess Cs(+) conditions in Escherichia coli
title Kup-mediated Cs(+) uptake and Kdp-driven K(+) uptake coordinate to promote cell growth during excess Cs(+) conditions in Escherichia coli
title_full Kup-mediated Cs(+) uptake and Kdp-driven K(+) uptake coordinate to promote cell growth during excess Cs(+) conditions in Escherichia coli
title_fullStr Kup-mediated Cs(+) uptake and Kdp-driven K(+) uptake coordinate to promote cell growth during excess Cs(+) conditions in Escherichia coli
title_full_unstemmed Kup-mediated Cs(+) uptake and Kdp-driven K(+) uptake coordinate to promote cell growth during excess Cs(+) conditions in Escherichia coli
title_short Kup-mediated Cs(+) uptake and Kdp-driven K(+) uptake coordinate to promote cell growth during excess Cs(+) conditions in Escherichia coli
title_sort kup-mediated cs(+) uptake and kdp-driven k(+) uptake coordinate to promote cell growth during excess cs(+) conditions in escherichia coli
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5437092/
https://www.ncbi.nlm.nih.gov/pubmed/28522840
http://dx.doi.org/10.1038/s41598-017-02164-7
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