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Genome-wide analysis of expansin superfamily in wild Arachis discloses a stress-responsive expansin-like B gene
Expansins are plant cell wall-loosening proteins involved in adaptive responses to environmental stimuli and various developmental processes. The first genome-wide analysis of the expansin superfamily in the Arachis genus identified 40 members in A. duranensis and 44 in A. ipaënsis, the wild progeni...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5437183/ https://www.ncbi.nlm.nih.gov/pubmed/28243841 http://dx.doi.org/10.1007/s11103-017-0594-8 |
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author | Guimaraes, Larissa Arrais Mota, Ana Paula Zotta Araujo, Ana Claudia Guerra de Alencar Figueiredo, Lucio Flavio Pereira, Bruna Medeiros de Passos Saraiva, Mario Alfredo Silva, Raquel Bispo Danchin, Etienne G. J. Guimaraes, Patricia Messenberg Brasileiro, Ana Cristina Miranda |
author_facet | Guimaraes, Larissa Arrais Mota, Ana Paula Zotta Araujo, Ana Claudia Guerra de Alencar Figueiredo, Lucio Flavio Pereira, Bruna Medeiros de Passos Saraiva, Mario Alfredo Silva, Raquel Bispo Danchin, Etienne G. J. Guimaraes, Patricia Messenberg Brasileiro, Ana Cristina Miranda |
author_sort | Guimaraes, Larissa Arrais |
collection | PubMed |
description | Expansins are plant cell wall-loosening proteins involved in adaptive responses to environmental stimuli and various developmental processes. The first genome-wide analysis of the expansin superfamily in the Arachis genus identified 40 members in A. duranensis and 44 in A. ipaënsis, the wild progenitors of cultivated peanut (A. hypogaea). These expansins were further characterized regarding their subfamily classification, distribution along the genomes, duplication events, molecular structure, and phylogeny. A RNA-seq expression analysis in different Arachis species showed that the majority of these expansins are modulated in response to diverse stresses such as water deficit, root-knot nematode (RKN) infection, and UV exposure, with an expansin-like B gene (AraEXLB8) displaying a highly distinct stress-responsive expression profile. Further analysis of the AraEXLB8 coding sequences showed high conservation across the Arachis genotypes, with eight haplotypes identified. The modulation of AraEXLB8 expression in response to the aforementioned stresses was confirmed by qRT-PCR analysis in distinct Arachis genotypes, whilst in situ hybridization revealed transcripts in different root tissues according to the stress imposed. The overexpression of AraEXLB8 in soybean (Glycine max) composite plants remarkably decreased the number of galls in transformed hairy roots inoculated with RKN. This study improves the current understanding of the molecular evolution, divergence, and gene expression of expansins in Arachis, and provides molecular and functional insights into the role of expansin-like B, the less-studied plant expansin subfamily. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11103-017-0594-8) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5437183 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-54371832017-06-06 Genome-wide analysis of expansin superfamily in wild Arachis discloses a stress-responsive expansin-like B gene Guimaraes, Larissa Arrais Mota, Ana Paula Zotta Araujo, Ana Claudia Guerra de Alencar Figueiredo, Lucio Flavio Pereira, Bruna Medeiros de Passos Saraiva, Mario Alfredo Silva, Raquel Bispo Danchin, Etienne G. J. Guimaraes, Patricia Messenberg Brasileiro, Ana Cristina Miranda Plant Mol Biol Article Expansins are plant cell wall-loosening proteins involved in adaptive responses to environmental stimuli and various developmental processes. The first genome-wide analysis of the expansin superfamily in the Arachis genus identified 40 members in A. duranensis and 44 in A. ipaënsis, the wild progenitors of cultivated peanut (A. hypogaea). These expansins were further characterized regarding their subfamily classification, distribution along the genomes, duplication events, molecular structure, and phylogeny. A RNA-seq expression analysis in different Arachis species showed that the majority of these expansins are modulated in response to diverse stresses such as water deficit, root-knot nematode (RKN) infection, and UV exposure, with an expansin-like B gene (AraEXLB8) displaying a highly distinct stress-responsive expression profile. Further analysis of the AraEXLB8 coding sequences showed high conservation across the Arachis genotypes, with eight haplotypes identified. The modulation of AraEXLB8 expression in response to the aforementioned stresses was confirmed by qRT-PCR analysis in distinct Arachis genotypes, whilst in situ hybridization revealed transcripts in different root tissues according to the stress imposed. The overexpression of AraEXLB8 in soybean (Glycine max) composite plants remarkably decreased the number of galls in transformed hairy roots inoculated with RKN. This study improves the current understanding of the molecular evolution, divergence, and gene expression of expansins in Arachis, and provides molecular and functional insights into the role of expansin-like B, the less-studied plant expansin subfamily. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11103-017-0594-8) contains supplementary material, which is available to authorized users. Springer Netherlands 2017-02-27 2017 /pmc/articles/PMC5437183/ /pubmed/28243841 http://dx.doi.org/10.1007/s11103-017-0594-8 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Article Guimaraes, Larissa Arrais Mota, Ana Paula Zotta Araujo, Ana Claudia Guerra de Alencar Figueiredo, Lucio Flavio Pereira, Bruna Medeiros de Passos Saraiva, Mario Alfredo Silva, Raquel Bispo Danchin, Etienne G. J. Guimaraes, Patricia Messenberg Brasileiro, Ana Cristina Miranda Genome-wide analysis of expansin superfamily in wild Arachis discloses a stress-responsive expansin-like B gene |
title | Genome-wide analysis of expansin superfamily in wild Arachis discloses a stress-responsive expansin-like B gene |
title_full | Genome-wide analysis of expansin superfamily in wild Arachis discloses a stress-responsive expansin-like B gene |
title_fullStr | Genome-wide analysis of expansin superfamily in wild Arachis discloses a stress-responsive expansin-like B gene |
title_full_unstemmed | Genome-wide analysis of expansin superfamily in wild Arachis discloses a stress-responsive expansin-like B gene |
title_short | Genome-wide analysis of expansin superfamily in wild Arachis discloses a stress-responsive expansin-like B gene |
title_sort | genome-wide analysis of expansin superfamily in wild arachis discloses a stress-responsive expansin-like b gene |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5437183/ https://www.ncbi.nlm.nih.gov/pubmed/28243841 http://dx.doi.org/10.1007/s11103-017-0594-8 |
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