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PPKs mediate direct signal transfer from phytochrome photoreceptors to transcription factor PIF3
Upon light-induced nuclear translocation, phytochrome (phy) sensory photoreceptors interact with, and induce rapid phosphorylation and consequent ubiquitin-mediated degradation of, transcription factors, called PIFs, thereby regulating target gene expression and plant development. Nevertheless, the...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5437280/ https://www.ncbi.nlm.nih.gov/pubmed/28492231 http://dx.doi.org/10.1038/ncomms15236 |
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author | Ni, Weimin Xu, Shou-Ling González-Grandío, Eduardo Chalkley, Robert J. Huhmer, Andreas F. R. Burlingame, Alma L. Wang, Zhi-Yong Quail, Peter H. |
author_facet | Ni, Weimin Xu, Shou-Ling González-Grandío, Eduardo Chalkley, Robert J. Huhmer, Andreas F. R. Burlingame, Alma L. Wang, Zhi-Yong Quail, Peter H. |
author_sort | Ni, Weimin |
collection | PubMed |
description | Upon light-induced nuclear translocation, phytochrome (phy) sensory photoreceptors interact with, and induce rapid phosphorylation and consequent ubiquitin-mediated degradation of, transcription factors, called PIFs, thereby regulating target gene expression and plant development. Nevertheless, the biochemical mechanism of phy-induced PIF phosphorylation has remained ill-defined. Here we identify a family of nuclear protein kinases, designated Photoregulatory Protein Kinases (PPK1–4; formerly called MUT9-Like Kinases (MLKs)), that interact with PIF3 and phyB in a light-induced manner in vivo. Genetic analyses demonstrate that the PPKs are collectively necessary for the normal light-induced phosphorylation and degradation of PIF3. PPK1 directly phosphorylates PIF3 in vitro, with a phosphosite pattern that strongly mimics the light-induced pattern in vivo. These data establish that the PPKs are directly involved in catalysing the photoactivated-phy-induced phosphorylation of PIF3 in vivo, and thereby are critical components of a transcriptionally centred signalling hub that pleiotropically regulates plant growth and development in response to multiple signalling pathways. |
format | Online Article Text |
id | pubmed-5437280 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-54372802017-06-01 PPKs mediate direct signal transfer from phytochrome photoreceptors to transcription factor PIF3 Ni, Weimin Xu, Shou-Ling González-Grandío, Eduardo Chalkley, Robert J. Huhmer, Andreas F. R. Burlingame, Alma L. Wang, Zhi-Yong Quail, Peter H. Nat Commun Article Upon light-induced nuclear translocation, phytochrome (phy) sensory photoreceptors interact with, and induce rapid phosphorylation and consequent ubiquitin-mediated degradation of, transcription factors, called PIFs, thereby regulating target gene expression and plant development. Nevertheless, the biochemical mechanism of phy-induced PIF phosphorylation has remained ill-defined. Here we identify a family of nuclear protein kinases, designated Photoregulatory Protein Kinases (PPK1–4; formerly called MUT9-Like Kinases (MLKs)), that interact with PIF3 and phyB in a light-induced manner in vivo. Genetic analyses demonstrate that the PPKs are collectively necessary for the normal light-induced phosphorylation and degradation of PIF3. PPK1 directly phosphorylates PIF3 in vitro, with a phosphosite pattern that strongly mimics the light-induced pattern in vivo. These data establish that the PPKs are directly involved in catalysing the photoactivated-phy-induced phosphorylation of PIF3 in vivo, and thereby are critical components of a transcriptionally centred signalling hub that pleiotropically regulates plant growth and development in response to multiple signalling pathways. Nature Publishing Group 2017-05-11 /pmc/articles/PMC5437280/ /pubmed/28492231 http://dx.doi.org/10.1038/ncomms15236 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Ni, Weimin Xu, Shou-Ling González-Grandío, Eduardo Chalkley, Robert J. Huhmer, Andreas F. R. Burlingame, Alma L. Wang, Zhi-Yong Quail, Peter H. PPKs mediate direct signal transfer from phytochrome photoreceptors to transcription factor PIF3 |
title | PPKs mediate direct signal transfer from phytochrome photoreceptors to transcription factor PIF3 |
title_full | PPKs mediate direct signal transfer from phytochrome photoreceptors to transcription factor PIF3 |
title_fullStr | PPKs mediate direct signal transfer from phytochrome photoreceptors to transcription factor PIF3 |
title_full_unstemmed | PPKs mediate direct signal transfer from phytochrome photoreceptors to transcription factor PIF3 |
title_short | PPKs mediate direct signal transfer from phytochrome photoreceptors to transcription factor PIF3 |
title_sort | ppks mediate direct signal transfer from phytochrome photoreceptors to transcription factor pif3 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5437280/ https://www.ncbi.nlm.nih.gov/pubmed/28492231 http://dx.doi.org/10.1038/ncomms15236 |
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