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High density lipoprotein promoting proliferation and migration of type II alveolar epithelial cells during inflammation state
BACKGROUND: To investigate the effect and mechanism of high density lipoprotein (HDL) on type II alveolar epithelial cells during inflammation state. METHODS: The original generation of type II alveolar epithelial cells were separated in rats and treated with PBS/LPS/HDL/HDL + LPS. To observe the pr...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5437408/ https://www.ncbi.nlm.nih.gov/pubmed/28521806 http://dx.doi.org/10.1186/s12944-017-0482-x |
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author | Yu, Zhongji Jin, Jingru Wang, Yuhui Sun, Jian |
author_facet | Yu, Zhongji Jin, Jingru Wang, Yuhui Sun, Jian |
author_sort | Yu, Zhongji |
collection | PubMed |
description | BACKGROUND: To investigate the effect and mechanism of high density lipoprotein (HDL) on type II alveolar epithelial cells during inflammation state. METHODS: The original generation of type II alveolar epithelial cells were separated in rats and treated with PBS/LPS/HDL/HDL + LPS. To observe the proliferation and migration of type II alveolar epithelial cells with bromodeoxyuridine(BrdU) assay, transwell assay and wound healing experiments. In addition, western blot detected the expression of TP-binding cassette transporter A1 (ABCA1), cystic fibrosis transmembrane conductance regulator (CFTR) and the phosphorylation of AKT/extracellular signal-regulated kinase(ERK)/mitogen-activated protein kinase(MAPK). Enzyme-linked immunosorbent assay (ELISA) tested the secretion of tumor necrosis factor a(TNF-a)/interleukin 1a(IL-1a)/IL-6. RESULTS: HDL promoted the proliferation (↑17%, p < 0.001 HDL+ LPS vs. LPS) and migration (wounding healing: ↑93%, p < 0.001 HDL+ LPS vs. LPS; transwell migration: ↑154%, p < 0.001 HDL+ LPS vs. LPS) of type II alveolar epithelial cells. Furthermore, HDL increased the phosphorylation of MAPK, but not AKT/ERK. And HDL decreased the secretion of TNF-a (↓46%, p < 0.01 HDL+ LPS vs. LPS) and IL-1a (↓45%, p < 0.001 HDL+ LPS vs. LPS), but not IL-6. In addition, HDL up-regulated the expression of ABCAI (↑99%, p < 0.001 HDL vs. CON) and down-regulated the expression of CFTR (↓25%, p < 0.05 HDL vs. CON) in type II alveolar epithelial cells. CONCLUSIONS: HDL increases the phosphorylation of MAPK, which promotes the proliferation and migration of type II alveolar epithelial cells. And it decreased the secretion of TNF-a/IL-1a and the expression of CFTR. All these suggest that HDL plays an important role in anti-inflammatory effect in inflammation state of lung. |
format | Online Article Text |
id | pubmed-5437408 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-54374082017-05-19 High density lipoprotein promoting proliferation and migration of type II alveolar epithelial cells during inflammation state Yu, Zhongji Jin, Jingru Wang, Yuhui Sun, Jian Lipids Health Dis Research BACKGROUND: To investigate the effect and mechanism of high density lipoprotein (HDL) on type II alveolar epithelial cells during inflammation state. METHODS: The original generation of type II alveolar epithelial cells were separated in rats and treated with PBS/LPS/HDL/HDL + LPS. To observe the proliferation and migration of type II alveolar epithelial cells with bromodeoxyuridine(BrdU) assay, transwell assay and wound healing experiments. In addition, western blot detected the expression of TP-binding cassette transporter A1 (ABCA1), cystic fibrosis transmembrane conductance regulator (CFTR) and the phosphorylation of AKT/extracellular signal-regulated kinase(ERK)/mitogen-activated protein kinase(MAPK). Enzyme-linked immunosorbent assay (ELISA) tested the secretion of tumor necrosis factor a(TNF-a)/interleukin 1a(IL-1a)/IL-6. RESULTS: HDL promoted the proliferation (↑17%, p < 0.001 HDL+ LPS vs. LPS) and migration (wounding healing: ↑93%, p < 0.001 HDL+ LPS vs. LPS; transwell migration: ↑154%, p < 0.001 HDL+ LPS vs. LPS) of type II alveolar epithelial cells. Furthermore, HDL increased the phosphorylation of MAPK, but not AKT/ERK. And HDL decreased the secretion of TNF-a (↓46%, p < 0.01 HDL+ LPS vs. LPS) and IL-1a (↓45%, p < 0.001 HDL+ LPS vs. LPS), but not IL-6. In addition, HDL up-regulated the expression of ABCAI (↑99%, p < 0.001 HDL vs. CON) and down-regulated the expression of CFTR (↓25%, p < 0.05 HDL vs. CON) in type II alveolar epithelial cells. CONCLUSIONS: HDL increases the phosphorylation of MAPK, which promotes the proliferation and migration of type II alveolar epithelial cells. And it decreased the secretion of TNF-a/IL-1a and the expression of CFTR. All these suggest that HDL plays an important role in anti-inflammatory effect in inflammation state of lung. BioMed Central 2017-05-18 /pmc/articles/PMC5437408/ /pubmed/28521806 http://dx.doi.org/10.1186/s12944-017-0482-x Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Yu, Zhongji Jin, Jingru Wang, Yuhui Sun, Jian High density lipoprotein promoting proliferation and migration of type II alveolar epithelial cells during inflammation state |
title | High density lipoprotein promoting proliferation and migration of type II alveolar epithelial cells during inflammation state |
title_full | High density lipoprotein promoting proliferation and migration of type II alveolar epithelial cells during inflammation state |
title_fullStr | High density lipoprotein promoting proliferation and migration of type II alveolar epithelial cells during inflammation state |
title_full_unstemmed | High density lipoprotein promoting proliferation and migration of type II alveolar epithelial cells during inflammation state |
title_short | High density lipoprotein promoting proliferation and migration of type II alveolar epithelial cells during inflammation state |
title_sort | high density lipoprotein promoting proliferation and migration of type ii alveolar epithelial cells during inflammation state |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5437408/ https://www.ncbi.nlm.nih.gov/pubmed/28521806 http://dx.doi.org/10.1186/s12944-017-0482-x |
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