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High density lipoprotein promoting proliferation and migration of type II alveolar epithelial cells during inflammation state

BACKGROUND: To investigate the effect and mechanism of high density lipoprotein (HDL) on type II alveolar epithelial cells during inflammation state. METHODS: The original generation of type II alveolar epithelial cells were separated in rats and treated with PBS/LPS/HDL/HDL + LPS. To observe the pr...

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Autores principales: Yu, Zhongji, Jin, Jingru, Wang, Yuhui, Sun, Jian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5437408/
https://www.ncbi.nlm.nih.gov/pubmed/28521806
http://dx.doi.org/10.1186/s12944-017-0482-x
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author Yu, Zhongji
Jin, Jingru
Wang, Yuhui
Sun, Jian
author_facet Yu, Zhongji
Jin, Jingru
Wang, Yuhui
Sun, Jian
author_sort Yu, Zhongji
collection PubMed
description BACKGROUND: To investigate the effect and mechanism of high density lipoprotein (HDL) on type II alveolar epithelial cells during inflammation state. METHODS: The original generation of type II alveolar epithelial cells were separated in rats and treated with PBS/LPS/HDL/HDL + LPS. To observe the proliferation and migration of type II alveolar epithelial cells with bromodeoxyuridine(BrdU) assay, transwell assay and wound healing experiments. In addition, western blot detected the expression of TP-binding cassette transporter A1 (ABCA1), cystic fibrosis transmembrane conductance regulator (CFTR) and the phosphorylation of AKT/extracellular signal-regulated kinase(ERK)/mitogen-activated protein kinase(MAPK). Enzyme-linked immunosorbent assay (ELISA) tested the secretion of tumor necrosis factor a(TNF-a)/interleukin 1a(IL-1a)/IL-6. RESULTS: HDL promoted the proliferation (↑17%, p < 0.001 HDL+ LPS vs. LPS) and migration (wounding healing: ↑93%, p < 0.001 HDL+ LPS vs. LPS; transwell migration: ↑154%, p < 0.001 HDL+ LPS vs. LPS) of type II alveolar epithelial cells. Furthermore, HDL increased the phosphorylation of MAPK, but not AKT/ERK. And HDL decreased the secretion of TNF-a (↓46%, p < 0.01 HDL+ LPS vs. LPS) and IL-1a (↓45%, p < 0.001 HDL+ LPS vs. LPS), but not IL-6. In addition, HDL up-regulated the expression of ABCAI (↑99%, p < 0.001 HDL vs. CON) and down-regulated the expression of CFTR (↓25%, p < 0.05 HDL vs. CON) in type II alveolar epithelial cells. CONCLUSIONS: HDL increases the phosphorylation of MAPK, which promotes the proliferation and migration of type II alveolar epithelial cells. And it decreased the secretion of TNF-a/IL-1a and the expression of CFTR. All these suggest that HDL plays an important role in anti-inflammatory effect in inflammation state of lung.
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spelling pubmed-54374082017-05-19 High density lipoprotein promoting proliferation and migration of type II alveolar epithelial cells during inflammation state Yu, Zhongji Jin, Jingru Wang, Yuhui Sun, Jian Lipids Health Dis Research BACKGROUND: To investigate the effect and mechanism of high density lipoprotein (HDL) on type II alveolar epithelial cells during inflammation state. METHODS: The original generation of type II alveolar epithelial cells were separated in rats and treated with PBS/LPS/HDL/HDL + LPS. To observe the proliferation and migration of type II alveolar epithelial cells with bromodeoxyuridine(BrdU) assay, transwell assay and wound healing experiments. In addition, western blot detected the expression of TP-binding cassette transporter A1 (ABCA1), cystic fibrosis transmembrane conductance regulator (CFTR) and the phosphorylation of AKT/extracellular signal-regulated kinase(ERK)/mitogen-activated protein kinase(MAPK). Enzyme-linked immunosorbent assay (ELISA) tested the secretion of tumor necrosis factor a(TNF-a)/interleukin 1a(IL-1a)/IL-6. RESULTS: HDL promoted the proliferation (↑17%, p < 0.001 HDL+ LPS vs. LPS) and migration (wounding healing: ↑93%, p < 0.001 HDL+ LPS vs. LPS; transwell migration: ↑154%, p < 0.001 HDL+ LPS vs. LPS) of type II alveolar epithelial cells. Furthermore, HDL increased the phosphorylation of MAPK, but not AKT/ERK. And HDL decreased the secretion of TNF-a (↓46%, p < 0.01 HDL+ LPS vs. LPS) and IL-1a (↓45%, p < 0.001 HDL+ LPS vs. LPS), but not IL-6. In addition, HDL up-regulated the expression of ABCAI (↑99%, p < 0.001 HDL vs. CON) and down-regulated the expression of CFTR (↓25%, p < 0.05 HDL vs. CON) in type II alveolar epithelial cells. CONCLUSIONS: HDL increases the phosphorylation of MAPK, which promotes the proliferation and migration of type II alveolar epithelial cells. And it decreased the secretion of TNF-a/IL-1a and the expression of CFTR. All these suggest that HDL plays an important role in anti-inflammatory effect in inflammation state of lung. BioMed Central 2017-05-18 /pmc/articles/PMC5437408/ /pubmed/28521806 http://dx.doi.org/10.1186/s12944-017-0482-x Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Yu, Zhongji
Jin, Jingru
Wang, Yuhui
Sun, Jian
High density lipoprotein promoting proliferation and migration of type II alveolar epithelial cells during inflammation state
title High density lipoprotein promoting proliferation and migration of type II alveolar epithelial cells during inflammation state
title_full High density lipoprotein promoting proliferation and migration of type II alveolar epithelial cells during inflammation state
title_fullStr High density lipoprotein promoting proliferation and migration of type II alveolar epithelial cells during inflammation state
title_full_unstemmed High density lipoprotein promoting proliferation and migration of type II alveolar epithelial cells during inflammation state
title_short High density lipoprotein promoting proliferation and migration of type II alveolar epithelial cells during inflammation state
title_sort high density lipoprotein promoting proliferation and migration of type ii alveolar epithelial cells during inflammation state
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5437408/
https://www.ncbi.nlm.nih.gov/pubmed/28521806
http://dx.doi.org/10.1186/s12944-017-0482-x
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