Cargando…

Combination curcumin and vitamin E treatment attenuates diet-induced steatosis in Hfe(-/-) mice

AIM: To investigate the synergistic hepato-protective properties of curcumin and vitamin E in an Hfe(-/-) high calorie diet model of steatohepatitis. METHODS: Hfe(-/-) C57BL/6J mice were fed either a high calorie diet or a high calorie diet with 1 mg/g curcumin; 1.5 mg/g vitamin E; or combination of...

Descripción completa

Detalles Bibliográficos
Autores principales: Heritage, Mandy, Jaskowski, Lesley, Bridle, Kim, Campbell, Catherine, Briskey, David, Britton, Laurence, Fletcher, Linda, Vitetta, Luis, Subramaniam, V Nathan, Crawford, Darrell
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Baishideng Publishing Group Inc 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5437504/
https://www.ncbi.nlm.nih.gov/pubmed/28573069
http://dx.doi.org/10.4291/wjgp.v8.i2.67
_version_ 1783237597552705536
author Heritage, Mandy
Jaskowski, Lesley
Bridle, Kim
Campbell, Catherine
Briskey, David
Britton, Laurence
Fletcher, Linda
Vitetta, Luis
Subramaniam, V Nathan
Crawford, Darrell
author_facet Heritage, Mandy
Jaskowski, Lesley
Bridle, Kim
Campbell, Catherine
Briskey, David
Britton, Laurence
Fletcher, Linda
Vitetta, Luis
Subramaniam, V Nathan
Crawford, Darrell
author_sort Heritage, Mandy
collection PubMed
description AIM: To investigate the synergistic hepato-protective properties of curcumin and vitamin E in an Hfe(-/-) high calorie diet model of steatohepatitis. METHODS: Hfe(-/-) C57BL/6J mice were fed either a high calorie diet or a high calorie diet with 1 mg/g curcumin; 1.5 mg/g vitamin E; or combination of 1 mg/g curcumin + 1.5 mg/g vitamin E for 20 wk. Serum and liver tissue were collected at the completion of the experiment. Liver histology was graded by a pathologist for steatosis, inflammation and fibrosis. RNA and protein was extracted from liver tissue to examine gene and protein expression associated with fatty acid oxidation, mitochondrial biogenesis and oxidative stress pathways. RESULTS: Hfe(-/-) mice fed the high calorie diet developed steatohepatitis and pericentral fibrosis. Combination treatment with curcumin and vitamin E resulted in a greater reduction of percent steatosis than either vitamin E or curcumin therapy alone. Serum alanine aminotransferase and non-alcoholic fatty liver disease (NAFLD) activity score were decreased following combination therapy with curcumin and vitamin E compared with high calorie diet alone. No changes were observed in inflammatory or fibrosis markers following treatment. Epididymal fat pad weights were significantly reduced following combination therapy, however total body weight and liver weight were unchanged. Combination therapy increased the mRNA expression of AdipoR2, Ppar-α, Cpt1a, Nrf-1 and Tfb2m suggesting enhanced fatty acid oxidation and mitochondrial biogenesis. In addition, combination treatment resulted in increased catalase activity in Hfe(-/-) mice. CONCLUSION: Combination curcumin and vitamin E treatment decreases liver injury in this steatohepatitis model, indicating that combination therapy may be of value in NAFLD.
format Online
Article
Text
id pubmed-5437504
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Baishideng Publishing Group Inc
record_format MEDLINE/PubMed
spelling pubmed-54375042017-06-01 Combination curcumin and vitamin E treatment attenuates diet-induced steatosis in Hfe(-/-) mice Heritage, Mandy Jaskowski, Lesley Bridle, Kim Campbell, Catherine Briskey, David Britton, Laurence Fletcher, Linda Vitetta, Luis Subramaniam, V Nathan Crawford, Darrell World J Gastrointest Pathophysiol Basic Study AIM: To investigate the synergistic hepato-protective properties of curcumin and vitamin E in an Hfe(-/-) high calorie diet model of steatohepatitis. METHODS: Hfe(-/-) C57BL/6J mice were fed either a high calorie diet or a high calorie diet with 1 mg/g curcumin; 1.5 mg/g vitamin E; or combination of 1 mg/g curcumin + 1.5 mg/g vitamin E for 20 wk. Serum and liver tissue were collected at the completion of the experiment. Liver histology was graded by a pathologist for steatosis, inflammation and fibrosis. RNA and protein was extracted from liver tissue to examine gene and protein expression associated with fatty acid oxidation, mitochondrial biogenesis and oxidative stress pathways. RESULTS: Hfe(-/-) mice fed the high calorie diet developed steatohepatitis and pericentral fibrosis. Combination treatment with curcumin and vitamin E resulted in a greater reduction of percent steatosis than either vitamin E or curcumin therapy alone. Serum alanine aminotransferase and non-alcoholic fatty liver disease (NAFLD) activity score were decreased following combination therapy with curcumin and vitamin E compared with high calorie diet alone. No changes were observed in inflammatory or fibrosis markers following treatment. Epididymal fat pad weights were significantly reduced following combination therapy, however total body weight and liver weight were unchanged. Combination therapy increased the mRNA expression of AdipoR2, Ppar-α, Cpt1a, Nrf-1 and Tfb2m suggesting enhanced fatty acid oxidation and mitochondrial biogenesis. In addition, combination treatment resulted in increased catalase activity in Hfe(-/-) mice. CONCLUSION: Combination curcumin and vitamin E treatment decreases liver injury in this steatohepatitis model, indicating that combination therapy may be of value in NAFLD. Baishideng Publishing Group Inc 2017-05-15 2017-05-15 /pmc/articles/PMC5437504/ /pubmed/28573069 http://dx.doi.org/10.4291/wjgp.v8.i2.67 Text en ©The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved. http://creativecommons.org/licenses/by-nc/4.0/ Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
spellingShingle Basic Study
Heritage, Mandy
Jaskowski, Lesley
Bridle, Kim
Campbell, Catherine
Briskey, David
Britton, Laurence
Fletcher, Linda
Vitetta, Luis
Subramaniam, V Nathan
Crawford, Darrell
Combination curcumin and vitamin E treatment attenuates diet-induced steatosis in Hfe(-/-) mice
title Combination curcumin and vitamin E treatment attenuates diet-induced steatosis in Hfe(-/-) mice
title_full Combination curcumin and vitamin E treatment attenuates diet-induced steatosis in Hfe(-/-) mice
title_fullStr Combination curcumin and vitamin E treatment attenuates diet-induced steatosis in Hfe(-/-) mice
title_full_unstemmed Combination curcumin and vitamin E treatment attenuates diet-induced steatosis in Hfe(-/-) mice
title_short Combination curcumin and vitamin E treatment attenuates diet-induced steatosis in Hfe(-/-) mice
title_sort combination curcumin and vitamin e treatment attenuates diet-induced steatosis in hfe(-/-) mice
topic Basic Study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5437504/
https://www.ncbi.nlm.nih.gov/pubmed/28573069
http://dx.doi.org/10.4291/wjgp.v8.i2.67
work_keys_str_mv AT heritagemandy combinationcurcuminandvitaminetreatmentattenuatesdietinducedsteatosisinhfemice
AT jaskowskilesley combinationcurcuminandvitaminetreatmentattenuatesdietinducedsteatosisinhfemice
AT bridlekim combinationcurcuminandvitaminetreatmentattenuatesdietinducedsteatosisinhfemice
AT campbellcatherine combinationcurcuminandvitaminetreatmentattenuatesdietinducedsteatosisinhfemice
AT briskeydavid combinationcurcuminandvitaminetreatmentattenuatesdietinducedsteatosisinhfemice
AT brittonlaurence combinationcurcuminandvitaminetreatmentattenuatesdietinducedsteatosisinhfemice
AT fletcherlinda combinationcurcuminandvitaminetreatmentattenuatesdietinducedsteatosisinhfemice
AT vitettaluis combinationcurcuminandvitaminetreatmentattenuatesdietinducedsteatosisinhfemice
AT subramaniamvnathan combinationcurcuminandvitaminetreatmentattenuatesdietinducedsteatosisinhfemice
AT crawforddarrell combinationcurcuminandvitaminetreatmentattenuatesdietinducedsteatosisinhfemice