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Differences in serum and synovial CD4+ T cells and cytokine profiles to stratify patients with inflammatory osteoarthritis and rheumatoid arthritis
BACKGROUND: The aim was to investigate CD4(+)T-cell subsets, immune cells and their cytokine profiles in blood and synovial compartments in rheumatoid arthritis (RA) and inflammatory osteoarthritis (OA) to define specific immune signatures. METHODS: Peripheral blood, synovial fluid (SF) and synovial...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5437517/ https://www.ncbi.nlm.nih.gov/pubmed/28526072 http://dx.doi.org/10.1186/s13075-017-1305-1 |
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author | Penatti, Alessandra Facciotti, Federica De Matteis, Roberta Larghi, Paola Paroni, Moira Murgo, Antonella De Lucia, Orazio Pagani, Massimiliano Pierannunzii, Luca Truzzi, Marcello Ioan-Facsinay, Andreea Abrignani, Sergio Geginat, Jens Meroni, Pier Luigi |
author_facet | Penatti, Alessandra Facciotti, Federica De Matteis, Roberta Larghi, Paola Paroni, Moira Murgo, Antonella De Lucia, Orazio Pagani, Massimiliano Pierannunzii, Luca Truzzi, Marcello Ioan-Facsinay, Andreea Abrignani, Sergio Geginat, Jens Meroni, Pier Luigi |
author_sort | Penatti, Alessandra |
collection | PubMed |
description | BACKGROUND: The aim was to investigate CD4(+)T-cell subsets, immune cells and their cytokine profiles in blood and synovial compartments in rheumatoid arthritis (RA) and inflammatory osteoarthritis (OA) to define specific immune signatures. METHODS: Peripheral blood, synovial fluid (SF) and synovial membranes (SM) of RA and OA patients were analyzed. CD4(+)T-cell subset frequencies were determined by flow cytometry, and cytokine concentrations in serum and SF were measured by ELISA. RESULTS: In peripheral blood, OA patients had altered frequencies of regulatory T-cell subsets, and higher frequencies of Th17 and of Th1/17 cells than RA patients. In the synovial compartment of OA patients, conventional Th17 cells were largely excluded, while Th1/17 cells were enriched and more frequent than in RA patients. Conversely, in the synovial compartment of RA patients, regulatory T cells and Tfh cells were enriched and more frequent then in OA patients. IL-17 and Blys were increased both in serum and SF of RA patients, and correlated with autoantibodies and disease activity. Notably, Blys levels were already significantly elevated in RA patients with low disease activity score in 28 joints (DAS28) and without autoantibody positivity. CONCLUSIONS: Although patients with inflammatory OA have immune activation in the synovial compartment, they display different T-cell subset frequencies and cytokine profiles. Soluble mediators such as Blys might help to discriminate mild clinical forms of RA from inflammatory OA particularly at the onset of the disease. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13075-017-1305-1) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5437517 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-54375172017-05-19 Differences in serum and synovial CD4+ T cells and cytokine profiles to stratify patients with inflammatory osteoarthritis and rheumatoid arthritis Penatti, Alessandra Facciotti, Federica De Matteis, Roberta Larghi, Paola Paroni, Moira Murgo, Antonella De Lucia, Orazio Pagani, Massimiliano Pierannunzii, Luca Truzzi, Marcello Ioan-Facsinay, Andreea Abrignani, Sergio Geginat, Jens Meroni, Pier Luigi Arthritis Res Ther Research Article BACKGROUND: The aim was to investigate CD4(+)T-cell subsets, immune cells and their cytokine profiles in blood and synovial compartments in rheumatoid arthritis (RA) and inflammatory osteoarthritis (OA) to define specific immune signatures. METHODS: Peripheral blood, synovial fluid (SF) and synovial membranes (SM) of RA and OA patients were analyzed. CD4(+)T-cell subset frequencies were determined by flow cytometry, and cytokine concentrations in serum and SF were measured by ELISA. RESULTS: In peripheral blood, OA patients had altered frequencies of regulatory T-cell subsets, and higher frequencies of Th17 and of Th1/17 cells than RA patients. In the synovial compartment of OA patients, conventional Th17 cells were largely excluded, while Th1/17 cells were enriched and more frequent than in RA patients. Conversely, in the synovial compartment of RA patients, regulatory T cells and Tfh cells were enriched and more frequent then in OA patients. IL-17 and Blys were increased both in serum and SF of RA patients, and correlated with autoantibodies and disease activity. Notably, Blys levels were already significantly elevated in RA patients with low disease activity score in 28 joints (DAS28) and without autoantibody positivity. CONCLUSIONS: Although patients with inflammatory OA have immune activation in the synovial compartment, they display different T-cell subset frequencies and cytokine profiles. Soluble mediators such as Blys might help to discriminate mild clinical forms of RA from inflammatory OA particularly at the onset of the disease. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13075-017-1305-1) contains supplementary material, which is available to authorized users. BioMed Central 2017-05-19 2017 /pmc/articles/PMC5437517/ /pubmed/28526072 http://dx.doi.org/10.1186/s13075-017-1305-1 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Penatti, Alessandra Facciotti, Federica De Matteis, Roberta Larghi, Paola Paroni, Moira Murgo, Antonella De Lucia, Orazio Pagani, Massimiliano Pierannunzii, Luca Truzzi, Marcello Ioan-Facsinay, Andreea Abrignani, Sergio Geginat, Jens Meroni, Pier Luigi Differences in serum and synovial CD4+ T cells and cytokine profiles to stratify patients with inflammatory osteoarthritis and rheumatoid arthritis |
title | Differences in serum and synovial CD4+ T cells and cytokine profiles to stratify patients with inflammatory osteoarthritis and rheumatoid arthritis |
title_full | Differences in serum and synovial CD4+ T cells and cytokine profiles to stratify patients with inflammatory osteoarthritis and rheumatoid arthritis |
title_fullStr | Differences in serum and synovial CD4+ T cells and cytokine profiles to stratify patients with inflammatory osteoarthritis and rheumatoid arthritis |
title_full_unstemmed | Differences in serum and synovial CD4+ T cells and cytokine profiles to stratify patients with inflammatory osteoarthritis and rheumatoid arthritis |
title_short | Differences in serum and synovial CD4+ T cells and cytokine profiles to stratify patients with inflammatory osteoarthritis and rheumatoid arthritis |
title_sort | differences in serum and synovial cd4+ t cells and cytokine profiles to stratify patients with inflammatory osteoarthritis and rheumatoid arthritis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5437517/ https://www.ncbi.nlm.nih.gov/pubmed/28526072 http://dx.doi.org/10.1186/s13075-017-1305-1 |
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