Cargando…

Fine-tuning of gene expression dynamics by the Set2-Rpd3S pathway

RNA polymerase II-interacting the Set2 methyltransferase co-transcriptionally methylates histone H3 at lysine 36 within the body of genes. This modification facilitates histone deacetylation by Rpd3S HDAC in 3′ transcribed regions to suppress cryptic initiation and slow elongation. Although this pat...

Descripción completa

Detalles Bibliográficos
Autores principales: Lee, Bo Bae, Kim, Ji Hyun, Kim, TaeSoo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Biochemistry and Molecular Biology 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5437959/
https://www.ncbi.nlm.nih.gov/pubmed/28320505
http://dx.doi.org/10.5483/BMBRep.2017.50.4.049
_version_ 1783237681198661632
author Lee, Bo Bae
Kim, Ji Hyun
Kim, TaeSoo
author_facet Lee, Bo Bae
Kim, Ji Hyun
Kim, TaeSoo
author_sort Lee, Bo Bae
collection PubMed
description RNA polymerase II-interacting the Set2 methyltransferase co-transcriptionally methylates histone H3 at lysine 36 within the body of genes. This modification facilitates histone deacetylation by Rpd3S HDAC in 3′ transcribed regions to suppress cryptic initiation and slow elongation. Although this pathway is important for global deacetylation, no strong effects have been seen on genome-wide transcription under optimized laboratory conditions. In contrast, this pathway slows the kinetics of mRNA induction when target genes are induced upon environmental changes. Interestingly, a majority of Set2-repressed genes are overlapped by a lncRNA transcription that targets H3K36 methylation and deacetylation by Rpd3S HDAC to mRNA promoters. Furthermore, this pathway delays the induction of many cryptic transcripts upon environmental changes. Therefore, the Set2-Rpd3S HDAC pathway functions to fine-tune expression dynamics of mRNAs and ncRNAs.
format Online
Article
Text
id pubmed-5437959
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Korean Society for Biochemistry and Molecular Biology
record_format MEDLINE/PubMed
spelling pubmed-54379592017-06-01 Fine-tuning of gene expression dynamics by the Set2-Rpd3S pathway Lee, Bo Bae Kim, Ji Hyun Kim, TaeSoo BMB Rep Perspective RNA polymerase II-interacting the Set2 methyltransferase co-transcriptionally methylates histone H3 at lysine 36 within the body of genes. This modification facilitates histone deacetylation by Rpd3S HDAC in 3′ transcribed regions to suppress cryptic initiation and slow elongation. Although this pathway is important for global deacetylation, no strong effects have been seen on genome-wide transcription under optimized laboratory conditions. In contrast, this pathway slows the kinetics of mRNA induction when target genes are induced upon environmental changes. Interestingly, a majority of Set2-repressed genes are overlapped by a lncRNA transcription that targets H3K36 methylation and deacetylation by Rpd3S HDAC to mRNA promoters. Furthermore, this pathway delays the induction of many cryptic transcripts upon environmental changes. Therefore, the Set2-Rpd3S HDAC pathway functions to fine-tune expression dynamics of mRNAs and ncRNAs. Korean Society for Biochemistry and Molecular Biology 2017-04 2017-04-30 /pmc/articles/PMC5437959/ /pubmed/28320505 http://dx.doi.org/10.5483/BMBRep.2017.50.4.049 Text en Copyright © 2017 by the The Korean Society for Biochemistry and Molecular Biology http://creativecommons.org/licenses/by-nc/4.0 This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Perspective
Lee, Bo Bae
Kim, Ji Hyun
Kim, TaeSoo
Fine-tuning of gene expression dynamics by the Set2-Rpd3S pathway
title Fine-tuning of gene expression dynamics by the Set2-Rpd3S pathway
title_full Fine-tuning of gene expression dynamics by the Set2-Rpd3S pathway
title_fullStr Fine-tuning of gene expression dynamics by the Set2-Rpd3S pathway
title_full_unstemmed Fine-tuning of gene expression dynamics by the Set2-Rpd3S pathway
title_short Fine-tuning of gene expression dynamics by the Set2-Rpd3S pathway
title_sort fine-tuning of gene expression dynamics by the set2-rpd3s pathway
topic Perspective
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5437959/
https://www.ncbi.nlm.nih.gov/pubmed/28320505
http://dx.doi.org/10.5483/BMBRep.2017.50.4.049
work_keys_str_mv AT leebobae finetuningofgeneexpressiondynamicsbytheset2rpd3spathway
AT kimjihyun finetuningofgeneexpressiondynamicsbytheset2rpd3spathway
AT kimtaesoo finetuningofgeneexpressiondynamicsbytheset2rpd3spathway