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Transcriptomic analyses and leukocyte telomere length measurement in subjects exposed to severe recent stressful life events

Stressful life events occurring in adulthood have been found able to affect mood and behavior, thus increasing the vulnerability for several stress-related psychiatric disorders. However, although there is plenty of clinical data supporting an association between stressful life events in adulthood a...

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Autores principales: Lopizzo, N, Tosato, S, Begni, V, Tomassi, S, Cattane, N, Barcella, M, Turco, G, Ruggeri, M, Riva, M A, Pariante, C M, Cattaneo, A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5438034/
https://www.ncbi.nlm.nih.gov/pubmed/28221367
http://dx.doi.org/10.1038/tp.2017.5
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author Lopizzo, N
Tosato, S
Begni, V
Tomassi, S
Cattane, N
Barcella, M
Turco, G
Ruggeri, M
Riva, M A
Pariante, C M
Cattaneo, A
author_facet Lopizzo, N
Tosato, S
Begni, V
Tomassi, S
Cattane, N
Barcella, M
Turco, G
Ruggeri, M
Riva, M A
Pariante, C M
Cattaneo, A
author_sort Lopizzo, N
collection PubMed
description Stressful life events occurring in adulthood have been found able to affect mood and behavior, thus increasing the vulnerability for several stress-related psychiatric disorders. However, although there is plenty of clinical data supporting an association between stressful life events in adulthood and an enhanced vulnerability for psychopathology, the underlying molecular mechanisms are still poorly investigated. Thus, in this study we performed peripheral/whole-genome transcriptomic analyses in blood samples obtained from 53 adult subjects characterized for recent stressful life events occurred within the previous 6 months. Transcriptomic data were analyzed using Partek Genomics Suite; pathway and network analyses were performed using Ingenuity Pathway Analysis and GeneMANIA Software. We found 207 genes significantly differentially expressed in adult subjects who reported recent stressful life experiences (n=21) compared with those without such experiences (n=32). Moreover, the same subjects exposed to such stressful experiences showed a reduction in leukocyte telomere length. A correlation analyses between telomere length and transcriptomic data indicated an association between the exposures to recent stressful life events and the modulation of several pathways, mainly involved in immune-inflammatory-related processes and oxidative stress, such as natural killer cell signaling, interleukin-1 (IL-1) signaling, MIF regulation of innate immunity and IL-6 signaling. Our data suggest an association between exposures to recent stressful life events in adulthood and alterations in the immune, inflammatory and oxidative stress pathways, which could be also involved in the negative effect of stressful life events on leukocyte telomere length. The modulation of these mechanisms may underlie the clinical association between the exposure to recent Stressful life events in adulthood and an enhanced vulnerability to develop psychiatric diseases in adulthood.
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spelling pubmed-54380342017-06-01 Transcriptomic analyses and leukocyte telomere length measurement in subjects exposed to severe recent stressful life events Lopizzo, N Tosato, S Begni, V Tomassi, S Cattane, N Barcella, M Turco, G Ruggeri, M Riva, M A Pariante, C M Cattaneo, A Transl Psychiatry Original Article Stressful life events occurring in adulthood have been found able to affect mood and behavior, thus increasing the vulnerability for several stress-related psychiatric disorders. However, although there is plenty of clinical data supporting an association between stressful life events in adulthood and an enhanced vulnerability for psychopathology, the underlying molecular mechanisms are still poorly investigated. Thus, in this study we performed peripheral/whole-genome transcriptomic analyses in blood samples obtained from 53 adult subjects characterized for recent stressful life events occurred within the previous 6 months. Transcriptomic data were analyzed using Partek Genomics Suite; pathway and network analyses were performed using Ingenuity Pathway Analysis and GeneMANIA Software. We found 207 genes significantly differentially expressed in adult subjects who reported recent stressful life experiences (n=21) compared with those without such experiences (n=32). Moreover, the same subjects exposed to such stressful experiences showed a reduction in leukocyte telomere length. A correlation analyses between telomere length and transcriptomic data indicated an association between the exposures to recent stressful life events and the modulation of several pathways, mainly involved in immune-inflammatory-related processes and oxidative stress, such as natural killer cell signaling, interleukin-1 (IL-1) signaling, MIF regulation of innate immunity and IL-6 signaling. Our data suggest an association between exposures to recent stressful life events in adulthood and alterations in the immune, inflammatory and oxidative stress pathways, which could be also involved in the negative effect of stressful life events on leukocyte telomere length. The modulation of these mechanisms may underlie the clinical association between the exposure to recent Stressful life events in adulthood and an enhanced vulnerability to develop psychiatric diseases in adulthood. Nature Publishing Group 2017-02 2017-02-21 /pmc/articles/PMC5438034/ /pubmed/28221367 http://dx.doi.org/10.1038/tp.2017.5 Text en Copyright © 2017 The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Original Article
Lopizzo, N
Tosato, S
Begni, V
Tomassi, S
Cattane, N
Barcella, M
Turco, G
Ruggeri, M
Riva, M A
Pariante, C M
Cattaneo, A
Transcriptomic analyses and leukocyte telomere length measurement in subjects exposed to severe recent stressful life events
title Transcriptomic analyses and leukocyte telomere length measurement in subjects exposed to severe recent stressful life events
title_full Transcriptomic analyses and leukocyte telomere length measurement in subjects exposed to severe recent stressful life events
title_fullStr Transcriptomic analyses and leukocyte telomere length measurement in subjects exposed to severe recent stressful life events
title_full_unstemmed Transcriptomic analyses and leukocyte telomere length measurement in subjects exposed to severe recent stressful life events
title_short Transcriptomic analyses and leukocyte telomere length measurement in subjects exposed to severe recent stressful life events
title_sort transcriptomic analyses and leukocyte telomere length measurement in subjects exposed to severe recent stressful life events
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5438034/
https://www.ncbi.nlm.nih.gov/pubmed/28221367
http://dx.doi.org/10.1038/tp.2017.5
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