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Expansion of the redox sensitive proteome coincides with the plastid endosymbiosis

The redox sensitive proteome (RSP) consists of protein thiols, in which their biochemical characteristics changed upon oxidation, playing an important role in coordinating cellular processes. Here, we applied a large-scale phylogenomic reconstruction approach in the model diatom Phaeodactylum tricor...

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Autores principales: Woehle, Christian, Dagan, Tal, Landan, Giddy, Vardi, Assaf, Rosenwasser, Shilo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5438061/
https://www.ncbi.nlm.nih.gov/pubmed/28504699
http://dx.doi.org/10.1038/nplants.2017.66
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author Woehle, Christian
Dagan, Tal
Landan, Giddy
Vardi, Assaf
Rosenwasser, Shilo
author_facet Woehle, Christian
Dagan, Tal
Landan, Giddy
Vardi, Assaf
Rosenwasser, Shilo
author_sort Woehle, Christian
collection PubMed
description The redox sensitive proteome (RSP) consists of protein thiols, in which their biochemical characteristics changed upon oxidation, playing an important role in coordinating cellular processes. Here, we applied a large-scale phylogenomic reconstruction approach in the model diatom Phaeodactylum tricornutum to map the evolutionary origins of the eukaryotic RSP. The majority of P. tricornutum redox sensitive cysteines (76%) is specific to eukaryotes, yet these are encoded in genes that are mostly of a prokaryotic origin (57%). Furthermore, we find a three-fold enrichment in redox sensitive cysteines in genes that were gained by endosymbiotic gene transfer during the primary plastid acquisition. The secondary endosymbiosis event coincides with frequent introduction of reactive cysteines into existing proteins. While the plastid acquisition imposed an increase in the production of reactive oxygen species, our results suggest that it was accompanied by significant expansion of the RSP, providing redox regulatory networks to cope with fluctuated environmental conditions.
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spelling pubmed-54380612017-11-15 Expansion of the redox sensitive proteome coincides with the plastid endosymbiosis Woehle, Christian Dagan, Tal Landan, Giddy Vardi, Assaf Rosenwasser, Shilo Nat Plants Article The redox sensitive proteome (RSP) consists of protein thiols, in which their biochemical characteristics changed upon oxidation, playing an important role in coordinating cellular processes. Here, we applied a large-scale phylogenomic reconstruction approach in the model diatom Phaeodactylum tricornutum to map the evolutionary origins of the eukaryotic RSP. The majority of P. tricornutum redox sensitive cysteines (76%) is specific to eukaryotes, yet these are encoded in genes that are mostly of a prokaryotic origin (57%). Furthermore, we find a three-fold enrichment in redox sensitive cysteines in genes that were gained by endosymbiotic gene transfer during the primary plastid acquisition. The secondary endosymbiosis event coincides with frequent introduction of reactive cysteines into existing proteins. While the plastid acquisition imposed an increase in the production of reactive oxygen species, our results suggest that it was accompanied by significant expansion of the RSP, providing redox regulatory networks to cope with fluctuated environmental conditions. 2017-05-15 /pmc/articles/PMC5438061/ /pubmed/28504699 http://dx.doi.org/10.1038/nplants.2017.66 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Woehle, Christian
Dagan, Tal
Landan, Giddy
Vardi, Assaf
Rosenwasser, Shilo
Expansion of the redox sensitive proteome coincides with the plastid endosymbiosis
title Expansion of the redox sensitive proteome coincides with the plastid endosymbiosis
title_full Expansion of the redox sensitive proteome coincides with the plastid endosymbiosis
title_fullStr Expansion of the redox sensitive proteome coincides with the plastid endosymbiosis
title_full_unstemmed Expansion of the redox sensitive proteome coincides with the plastid endosymbiosis
title_short Expansion of the redox sensitive proteome coincides with the plastid endosymbiosis
title_sort expansion of the redox sensitive proteome coincides with the plastid endosymbiosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5438061/
https://www.ncbi.nlm.nih.gov/pubmed/28504699
http://dx.doi.org/10.1038/nplants.2017.66
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