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Evaluation of Arteriolar Smooth Muscle Cell Function in an Ex Vivo Microvascular Network Model

An emerging challenge in tissue engineering biomimetic models is recapitulating the physiological complexity associated with real tissues. Recently, our laboratory introduced the rat mesentery culture model as an ex vivo experimental platform for investigating the multi-cellular dynamics involved in...

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Autores principales: Motherwell, Jessica M., Azimi, Mohammad S., Spicer, Kristine, Alves, Natascha G., Hodges, Nicholas A., Breslin, Jerome W., Katakam, Prasad V. G., Murfee, Walter L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5438412/
https://www.ncbi.nlm.nih.gov/pubmed/28526859
http://dx.doi.org/10.1038/s41598-017-02272-4
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author Motherwell, Jessica M.
Azimi, Mohammad S.
Spicer, Kristine
Alves, Natascha G.
Hodges, Nicholas A.
Breslin, Jerome W.
Katakam, Prasad V. G.
Murfee, Walter L.
author_facet Motherwell, Jessica M.
Azimi, Mohammad S.
Spicer, Kristine
Alves, Natascha G.
Hodges, Nicholas A.
Breslin, Jerome W.
Katakam, Prasad V. G.
Murfee, Walter L.
author_sort Motherwell, Jessica M.
collection PubMed
description An emerging challenge in tissue engineering biomimetic models is recapitulating the physiological complexity associated with real tissues. Recently, our laboratory introduced the rat mesentery culture model as an ex vivo experimental platform for investigating the multi-cellular dynamics involved in angiogenesis within an intact microvascular network using time-lapse imaging. A critical question remains whether the vessels maintain their functionality. The objective of this study was to determine whether vascular smooth muscle cells in cultured microvascular networks maintain the ability to constrict. Adult rat mesenteric tissues were harvested and cultured for three days in either MEM or MEM plus 10% serum. On Day 0 and Day 3 live microvascular networks were visualized with FITC conjugated BSI-lectin labeling and arteriole diameters were compared before and five minutes after topical exposure to vasoconstrictors (50 mM KCl and 20 nM Endothelin-1). Arterioles displayed a vasoconstriction response to KCl and endothelin for each experimental group. However, the Day 3 serum cultured networks were angiogenic, characterized by increased vessel density, and displayed a decreased vasoconstriction response compared to Day 0 networks. The results support the physiological relevance of the rat mesentery culture model as a biomimetic tool for investigating microvascular growth and function ex vivo.
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spelling pubmed-54384122017-05-22 Evaluation of Arteriolar Smooth Muscle Cell Function in an Ex Vivo Microvascular Network Model Motherwell, Jessica M. Azimi, Mohammad S. Spicer, Kristine Alves, Natascha G. Hodges, Nicholas A. Breslin, Jerome W. Katakam, Prasad V. G. Murfee, Walter L. Sci Rep Article An emerging challenge in tissue engineering biomimetic models is recapitulating the physiological complexity associated with real tissues. Recently, our laboratory introduced the rat mesentery culture model as an ex vivo experimental platform for investigating the multi-cellular dynamics involved in angiogenesis within an intact microvascular network using time-lapse imaging. A critical question remains whether the vessels maintain their functionality. The objective of this study was to determine whether vascular smooth muscle cells in cultured microvascular networks maintain the ability to constrict. Adult rat mesenteric tissues were harvested and cultured for three days in either MEM or MEM plus 10% serum. On Day 0 and Day 3 live microvascular networks were visualized with FITC conjugated BSI-lectin labeling and arteriole diameters were compared before and five minutes after topical exposure to vasoconstrictors (50 mM KCl and 20 nM Endothelin-1). Arterioles displayed a vasoconstriction response to KCl and endothelin for each experimental group. However, the Day 3 serum cultured networks were angiogenic, characterized by increased vessel density, and displayed a decreased vasoconstriction response compared to Day 0 networks. The results support the physiological relevance of the rat mesentery culture model as a biomimetic tool for investigating microvascular growth and function ex vivo. Nature Publishing Group UK 2017-05-19 /pmc/articles/PMC5438412/ /pubmed/28526859 http://dx.doi.org/10.1038/s41598-017-02272-4 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Motherwell, Jessica M.
Azimi, Mohammad S.
Spicer, Kristine
Alves, Natascha G.
Hodges, Nicholas A.
Breslin, Jerome W.
Katakam, Prasad V. G.
Murfee, Walter L.
Evaluation of Arteriolar Smooth Muscle Cell Function in an Ex Vivo Microvascular Network Model
title Evaluation of Arteriolar Smooth Muscle Cell Function in an Ex Vivo Microvascular Network Model
title_full Evaluation of Arteriolar Smooth Muscle Cell Function in an Ex Vivo Microvascular Network Model
title_fullStr Evaluation of Arteriolar Smooth Muscle Cell Function in an Ex Vivo Microvascular Network Model
title_full_unstemmed Evaluation of Arteriolar Smooth Muscle Cell Function in an Ex Vivo Microvascular Network Model
title_short Evaluation of Arteriolar Smooth Muscle Cell Function in an Ex Vivo Microvascular Network Model
title_sort evaluation of arteriolar smooth muscle cell function in an ex vivo microvascular network model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5438412/
https://www.ncbi.nlm.nih.gov/pubmed/28526859
http://dx.doi.org/10.1038/s41598-017-02272-4
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