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Multiplex, DNase-free one-step reverse transcription PCR for Plasmodium 18S rRNA and spliced gametocyte-specific mRNAs

BACKGROUND: Plasmodium gametocytes are sexual stages transmitted to female Anopheles mosquitoes. While Plasmodium parasites can be differentiated microscopically on Giemsa-stained blood smears, molecular methods are increasingly used because of their increased sensitivity. Molecular detection of gam...

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Detalles Bibliográficos
Autores principales: Hanron, Amelia E., Billman, Zachary P., Seilie, Annette M., Olsen, Tayla M., Fishbaugher, Matthew, Chang, Ming, Rueckle, Thomas, Andenmatten, Nicole, Greenhouse, Bryan, Arinaitwe, Emmanuel, Rek, John, Das, Smita, Domingo, Gonzalo J., Shipman, Kelly, Kappe, Stefan H., Kublin, James G., Murphy, Sean C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5438523/
https://www.ncbi.nlm.nih.gov/pubmed/28526032
http://dx.doi.org/10.1186/s12936-017-1863-3