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Genetic Gains in Grain Yield of a Maize Population Improved through Marker Assisted Recurrent Selection under Stress and Non-stress Conditions in West Africa

Marker-assisted recurrent selection (MARS) is a breeding method used to accumulate favorable alleles that for example confer tolerance to drought in inbred lines from several genomic regions within a single population. A bi-parental cross formed from two parents that combine resistance to Striga her...

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Autores principales: Abdulmalik, Rekiya O., Menkir, Abebe, Meseka, Silvestro K., Unachukwu, Nnanna, Ado, Shehu G., Olarewaju, Joseph D., Aba, Daniel A., Hearne, Sarah, Crossa, Jose, Gedil, Melaku
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5438988/
https://www.ncbi.nlm.nih.gov/pubmed/28588598
http://dx.doi.org/10.3389/fpls.2017.00841
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author Abdulmalik, Rekiya O.
Menkir, Abebe
Meseka, Silvestro K.
Unachukwu, Nnanna
Ado, Shehu G.
Olarewaju, Joseph D.
Aba, Daniel A.
Hearne, Sarah
Crossa, Jose
Gedil, Melaku
author_facet Abdulmalik, Rekiya O.
Menkir, Abebe
Meseka, Silvestro K.
Unachukwu, Nnanna
Ado, Shehu G.
Olarewaju, Joseph D.
Aba, Daniel A.
Hearne, Sarah
Crossa, Jose
Gedil, Melaku
author_sort Abdulmalik, Rekiya O.
collection PubMed
description Marker-assisted recurrent selection (MARS) is a breeding method used to accumulate favorable alleles that for example confer tolerance to drought in inbred lines from several genomic regions within a single population. A bi-parental cross formed from two parents that combine resistance to Striga hermonthica with drought tolerance, which was improved through MARS, was used to assess changes in the frequency of favorable alleles and its impact on inbred line improvement. A total of 200 testcrosses of randomly selected S(1) lines derived from the original (C(0)) and advanced selection cycles of this bi-parental population, were evaluated under drought stress (DS) and well-watered (WW) conditions at Ikenne and under artificial Striga infestation at Abuja and Mokwa in Nigeria in 2014 and 2015. Also, 60 randomly selected S(1) lines each derived from the four cycles (C(0), C(1), C(2), C(3)) were genotyped with 233 SNP markers using KASP assay. The results showed that the frequency of favorable alleles increased with MARS in the bi-parental population with none of the markers showing fixation. The gain in grain yield was not significant under DS condition due to the combined effect of DS and armyworm infestation in 2015. Because the parents used for developing the bi-parental cross combined tolerance to drought with resistance to Striga, improvement in grain yield under DS did not result in undesirable changes in resistance to the parasite in the bi-parental maize population improved through MARS. MARS increased the mean number of combinations of favorable alleles in S(1) lines from 114 in C(0) to 124 in C(3). The level of heterozygosity decreased by 15%, while homozygosity increased by 13% due to the loss of some genotypes in the population. This study demonstrated the effectiveness of MARS in increasing the frequency of favorable alleles for tolerance to drought without disrupting the level of resistance to Striga in a bi-parental population targeted as a source of improved maize inbred lines.
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spelling pubmed-54389882017-06-06 Genetic Gains in Grain Yield of a Maize Population Improved through Marker Assisted Recurrent Selection under Stress and Non-stress Conditions in West Africa Abdulmalik, Rekiya O. Menkir, Abebe Meseka, Silvestro K. Unachukwu, Nnanna Ado, Shehu G. Olarewaju, Joseph D. Aba, Daniel A. Hearne, Sarah Crossa, Jose Gedil, Melaku Front Plant Sci Plant Science Marker-assisted recurrent selection (MARS) is a breeding method used to accumulate favorable alleles that for example confer tolerance to drought in inbred lines from several genomic regions within a single population. A bi-parental cross formed from two parents that combine resistance to Striga hermonthica with drought tolerance, which was improved through MARS, was used to assess changes in the frequency of favorable alleles and its impact on inbred line improvement. A total of 200 testcrosses of randomly selected S(1) lines derived from the original (C(0)) and advanced selection cycles of this bi-parental population, were evaluated under drought stress (DS) and well-watered (WW) conditions at Ikenne and under artificial Striga infestation at Abuja and Mokwa in Nigeria in 2014 and 2015. Also, 60 randomly selected S(1) lines each derived from the four cycles (C(0), C(1), C(2), C(3)) were genotyped with 233 SNP markers using KASP assay. The results showed that the frequency of favorable alleles increased with MARS in the bi-parental population with none of the markers showing fixation. The gain in grain yield was not significant under DS condition due to the combined effect of DS and armyworm infestation in 2015. Because the parents used for developing the bi-parental cross combined tolerance to drought with resistance to Striga, improvement in grain yield under DS did not result in undesirable changes in resistance to the parasite in the bi-parental maize population improved through MARS. MARS increased the mean number of combinations of favorable alleles in S(1) lines from 114 in C(0) to 124 in C(3). The level of heterozygosity decreased by 15%, while homozygosity increased by 13% due to the loss of some genotypes in the population. This study demonstrated the effectiveness of MARS in increasing the frequency of favorable alleles for tolerance to drought without disrupting the level of resistance to Striga in a bi-parental population targeted as a source of improved maize inbred lines. Frontiers Media S.A. 2017-05-22 /pmc/articles/PMC5438988/ /pubmed/28588598 http://dx.doi.org/10.3389/fpls.2017.00841 Text en Copyright © 2017 Abdulmalik, Menkir, Meseka, Unachukwu, Ado, Olarewaju, Aba, Hearne, Crossa and Gedil. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Abdulmalik, Rekiya O.
Menkir, Abebe
Meseka, Silvestro K.
Unachukwu, Nnanna
Ado, Shehu G.
Olarewaju, Joseph D.
Aba, Daniel A.
Hearne, Sarah
Crossa, Jose
Gedil, Melaku
Genetic Gains in Grain Yield of a Maize Population Improved through Marker Assisted Recurrent Selection under Stress and Non-stress Conditions in West Africa
title Genetic Gains in Grain Yield of a Maize Population Improved through Marker Assisted Recurrent Selection under Stress and Non-stress Conditions in West Africa
title_full Genetic Gains in Grain Yield of a Maize Population Improved through Marker Assisted Recurrent Selection under Stress and Non-stress Conditions in West Africa
title_fullStr Genetic Gains in Grain Yield of a Maize Population Improved through Marker Assisted Recurrent Selection under Stress and Non-stress Conditions in West Africa
title_full_unstemmed Genetic Gains in Grain Yield of a Maize Population Improved through Marker Assisted Recurrent Selection under Stress and Non-stress Conditions in West Africa
title_short Genetic Gains in Grain Yield of a Maize Population Improved through Marker Assisted Recurrent Selection under Stress and Non-stress Conditions in West Africa
title_sort genetic gains in grain yield of a maize population improved through marker assisted recurrent selection under stress and non-stress conditions in west africa
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5438988/
https://www.ncbi.nlm.nih.gov/pubmed/28588598
http://dx.doi.org/10.3389/fpls.2017.00841
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