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Diversity of Ligninolytic Enzymes and Their Genes in Strains of the Genus Ganoderma: Applicable for Biodegradation of Xenobiotic Compounds?

White-rot fungi (WRF) and their ligninolytic enzymes (laccases and peroxidases) are considered promising biotechnological tools to remove lignin related Persistent Organic Pollutants from industrial wastewaters and contaminated ecosystems. A high diversity of the genus Ganoderma has been reported in...

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Autores principales: Torres-Farradá, Giselle, Manzano León, Ana M., Rineau, François, Ledo Alonso, Lucía L., Sánchez-López, María I., Thijs, Sofie, Colpaert, Jan, Ramos-Leal, Miguel, Guerra, Gilda, Vangronsveld, Jaco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5440474/
https://www.ncbi.nlm.nih.gov/pubmed/28588565
http://dx.doi.org/10.3389/fmicb.2017.00898
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author Torres-Farradá, Giselle
Manzano León, Ana M.
Rineau, François
Ledo Alonso, Lucía L.
Sánchez-López, María I.
Thijs, Sofie
Colpaert, Jan
Ramos-Leal, Miguel
Guerra, Gilda
Vangronsveld, Jaco
author_facet Torres-Farradá, Giselle
Manzano León, Ana M.
Rineau, François
Ledo Alonso, Lucía L.
Sánchez-López, María I.
Thijs, Sofie
Colpaert, Jan
Ramos-Leal, Miguel
Guerra, Gilda
Vangronsveld, Jaco
author_sort Torres-Farradá, Giselle
collection PubMed
description White-rot fungi (WRF) and their ligninolytic enzymes (laccases and peroxidases) are considered promising biotechnological tools to remove lignin related Persistent Organic Pollutants from industrial wastewaters and contaminated ecosystems. A high diversity of the genus Ganoderma has been reported in Cuba; in spite of this, the diversity of ligninolytic enzymes and their genes remained unexplored. In this study, 13 native WRF strains were isolated from decayed wood in urban ecosystems in Havana (Cuba). All strains were identified as Ganoderma sp. using a multiplex polymerase chain reaction (PCR)-method based on ITS sequences. All Ganoderma sp. strains produced laccase enzymes at higher levels than non-specific peroxidases. Native-PAGE of extracellular enzymatic extracts revealed a high diversity of laccase isozymes patterns between the strains, suggesting the presence of different amino acid sequences in the laccase enzymes produced by these Ganoderma strains. We determined the diversity of genes encoding laccases and peroxidases using a PCR and cloning approach with basidiomycete-specific primers. Between two and five laccase genes were detected in each strain. In contrast, only one gene encoding manganese peroxidase or versatile peroxidase was detected in each strain. The translated laccases and peroxidases amino acid sequences have not been described before. Extracellular crude enzymatic extracts produced by the Ganoderma UH strains, were able to degrade model chromophoric compounds such as anthraquinone and azo dyes. These findings hold promises for the development of a practical application for the treatment of textile industry wastewaters and also for bioremediation of polluted ecosystems by well-adapted native WRF strains.
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spelling pubmed-54404742017-06-06 Diversity of Ligninolytic Enzymes and Their Genes in Strains of the Genus Ganoderma: Applicable for Biodegradation of Xenobiotic Compounds? Torres-Farradá, Giselle Manzano León, Ana M. Rineau, François Ledo Alonso, Lucía L. Sánchez-López, María I. Thijs, Sofie Colpaert, Jan Ramos-Leal, Miguel Guerra, Gilda Vangronsveld, Jaco Front Microbiol Microbiology White-rot fungi (WRF) and their ligninolytic enzymes (laccases and peroxidases) are considered promising biotechnological tools to remove lignin related Persistent Organic Pollutants from industrial wastewaters and contaminated ecosystems. A high diversity of the genus Ganoderma has been reported in Cuba; in spite of this, the diversity of ligninolytic enzymes and their genes remained unexplored. In this study, 13 native WRF strains were isolated from decayed wood in urban ecosystems in Havana (Cuba). All strains were identified as Ganoderma sp. using a multiplex polymerase chain reaction (PCR)-method based on ITS sequences. All Ganoderma sp. strains produced laccase enzymes at higher levels than non-specific peroxidases. Native-PAGE of extracellular enzymatic extracts revealed a high diversity of laccase isozymes patterns between the strains, suggesting the presence of different amino acid sequences in the laccase enzymes produced by these Ganoderma strains. We determined the diversity of genes encoding laccases and peroxidases using a PCR and cloning approach with basidiomycete-specific primers. Between two and five laccase genes were detected in each strain. In contrast, only one gene encoding manganese peroxidase or versatile peroxidase was detected in each strain. The translated laccases and peroxidases amino acid sequences have not been described before. Extracellular crude enzymatic extracts produced by the Ganoderma UH strains, were able to degrade model chromophoric compounds such as anthraquinone and azo dyes. These findings hold promises for the development of a practical application for the treatment of textile industry wastewaters and also for bioremediation of polluted ecosystems by well-adapted native WRF strains. Frontiers Media S.A. 2017-05-23 /pmc/articles/PMC5440474/ /pubmed/28588565 http://dx.doi.org/10.3389/fmicb.2017.00898 Text en Copyright © 2017 Torres-Farradá, Manzano León, Rineau, Ledo Alonso, Sánchez-López, Thijs, Colpaert, Ramos-Leal, Guerra and Vangronsveld. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Torres-Farradá, Giselle
Manzano León, Ana M.
Rineau, François
Ledo Alonso, Lucía L.
Sánchez-López, María I.
Thijs, Sofie
Colpaert, Jan
Ramos-Leal, Miguel
Guerra, Gilda
Vangronsveld, Jaco
Diversity of Ligninolytic Enzymes and Their Genes in Strains of the Genus Ganoderma: Applicable for Biodegradation of Xenobiotic Compounds?
title Diversity of Ligninolytic Enzymes and Their Genes in Strains of the Genus Ganoderma: Applicable for Biodegradation of Xenobiotic Compounds?
title_full Diversity of Ligninolytic Enzymes and Their Genes in Strains of the Genus Ganoderma: Applicable for Biodegradation of Xenobiotic Compounds?
title_fullStr Diversity of Ligninolytic Enzymes and Their Genes in Strains of the Genus Ganoderma: Applicable for Biodegradation of Xenobiotic Compounds?
title_full_unstemmed Diversity of Ligninolytic Enzymes and Their Genes in Strains of the Genus Ganoderma: Applicable for Biodegradation of Xenobiotic Compounds?
title_short Diversity of Ligninolytic Enzymes and Their Genes in Strains of the Genus Ganoderma: Applicable for Biodegradation of Xenobiotic Compounds?
title_sort diversity of ligninolytic enzymes and their genes in strains of the genus ganoderma: applicable for biodegradation of xenobiotic compounds?
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5440474/
https://www.ncbi.nlm.nih.gov/pubmed/28588565
http://dx.doi.org/10.3389/fmicb.2017.00898
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