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Efficient Genome Editing of a Facultative Thermophile Using Mesophilic spCas9

[Image: see text] Well-developed genetic tools for thermophilic microorganisms are scarce, despite their industrial and scientific relevance. Whereas highly efficient CRISPR/Cas9-based genome editing is on the rise in prokaryotes, it has never been employed in a thermophile. Here, we apply Streptoco...

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Detalles Bibliográficos
Autores principales:	Mougiakos, Ioannis, Bosma, Elleke F., Weenink, Koen, Vossen, Eric, Goijvaerts, Kirsten, van der Oost, John, van Kranenburg, Richard
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Chemical Society 2017
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5440800/ https://www.ncbi.nlm.nih.gov/pubmed/28146359 http://dx.doi.org/10.1021/acssynbio.6b00339

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