Nasosorption as a Minimally Invasive Sampling Procedure: Mucosal Viral Load and Inflammation in Primary RSV Bronchiolitis

BACKGROUND. Existing respiratory mucosal sampling methods are flawed, particularly in a pediatric bronchiolitis setting. METHODS. Twenty-four infants with bronchiolitis were recruited: 12 were respiratory syncytial virus (RSV)–positive, 12 were RSV-negative. Infants were sampled by nasosorption and...

Descripción completa

Detalles Bibliográficos
Autores principales: Thwaites, Ryan S., Ito, Kazuhiro, Chingono, Jasmine M. S., Coates, Matthew, Jarvis, Hannah C., Tunstall, Tanushree, Anderson-Dring, Lauren, Cass, Lindsey, Rapeport, Garth, Openshaw, Peter J., Nadel, Simon, Hansel, Trevor T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5441107/
https://www.ncbi.nlm.nih.gov/pubmed/28368490
http://dx.doi.org/10.1093/infdis/jix150
Descripción
Sumario:BACKGROUND. Existing respiratory mucosal sampling methods are flawed, particularly in a pediatric bronchiolitis setting. METHODS. Twenty-four infants with bronchiolitis were recruited: 12 were respiratory syncytial virus (RSV)–positive, 12 were RSV-negative. Infants were sampled by nasosorption and nasopharyngeal aspiration (NPA). RESULTS. Nasosorption was well tolerated and identified all RSV+ samples. RSV load measured by nasosorption (but not NPA) correlated with length of hospital stay (P = .04) and requirement for mechanical ventilation (P = .03). Nasosorption (but not NPA) levels of interferon γ, interleukin 1β, CCL5/RANTES, and interleukin 10 (IL-10) were elevated in RSV+ bronchiolitis (all P < .05), furthermore CCL5 and IL-10 correlated with RSV load (P < .05). CONCLUSIONS. Nasosorption allowed measurement of RSV load and the mucosal inflammatory response in infants.

Ejemplares similares