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Label‐Free and Regenerative Electrochemical Microfluidic Biosensors for Continual Monitoring of Cell Secretomes

Development of an efficient sensing platform capable of continual monitoring of biomarkers is needed to assess the functionality of the in vitro organoids and to evaluate their biological responses toward pharmaceutical compounds or chemical species over extended periods of time. Here, a novel label...

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Autores principales: Shin, Su Ryon, Kilic, Tugba, Zhang, Yu Shrike, Avci, Huseyin, Hu, Ning, Kim, Duckjin, Branco, Cristina, Aleman, Julio, Massa, Solange, Silvestri, Antonia, Kang, Jian, Desalvo, Anna, Hussaini, Mohammed Abdullah, Chae, Su‐Kyoung, Polini, Alessandro, Bhise, Nupura, Hussain, Mohammad Asif, Lee, HeaYeon, Dokmeci, Mehmet R., Khademhosseini, Ali
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5441508/
https://www.ncbi.nlm.nih.gov/pubmed/28546915
http://dx.doi.org/10.1002/advs.201600522
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author Shin, Su Ryon
Kilic, Tugba
Zhang, Yu Shrike
Avci, Huseyin
Hu, Ning
Kim, Duckjin
Branco, Cristina
Aleman, Julio
Massa, Solange
Silvestri, Antonia
Kang, Jian
Desalvo, Anna
Hussaini, Mohammed Abdullah
Chae, Su‐Kyoung
Polini, Alessandro
Bhise, Nupura
Hussain, Mohammad Asif
Lee, HeaYeon
Dokmeci, Mehmet R.
Khademhosseini, Ali
author_facet Shin, Su Ryon
Kilic, Tugba
Zhang, Yu Shrike
Avci, Huseyin
Hu, Ning
Kim, Duckjin
Branco, Cristina
Aleman, Julio
Massa, Solange
Silvestri, Antonia
Kang, Jian
Desalvo, Anna
Hussaini, Mohammed Abdullah
Chae, Su‐Kyoung
Polini, Alessandro
Bhise, Nupura
Hussain, Mohammad Asif
Lee, HeaYeon
Dokmeci, Mehmet R.
Khademhosseini, Ali
author_sort Shin, Su Ryon
collection PubMed
description Development of an efficient sensing platform capable of continual monitoring of biomarkers is needed to assess the functionality of the in vitro organoids and to evaluate their biological responses toward pharmaceutical compounds or chemical species over extended periods of time. Here, a novel label‐free microfluidic electrochemical (EC) biosensor with a unique built‐in on‐chip regeneration capability for continual measurement of cell‐secreted soluble biomarkers from an organoid culture in a fully automated manner without attenuating the sensor sensitivity is reported. The microfluidic EC biosensors are integrated with a human liver‐on‐a‐chip platform for continual monitoring of the metabolic activity of the organoids by measuring the levels of secreted biomarkers for up to 7 d, where the metabolic activity of the organoids is altered by a systemically applied drug. The variations in the biomarker levels are successfully measured by the microfluidic regenerative EC biosensors and agree well with cellular viability and enzyme‐linked immunosorbent assay analyses, validating the accuracy of the unique sensing platform. It is believed that this versatile and robust microfluidic EC biosensor that is capable of automated and continual detection of soluble biomarkers will find widespread use for long‐term monitoring of human organoids during drug toxicity studies or efficacy assessments of in vitro platforms.
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spelling pubmed-54415082017-05-25 Label‐Free and Regenerative Electrochemical Microfluidic Biosensors for Continual Monitoring of Cell Secretomes Shin, Su Ryon Kilic, Tugba Zhang, Yu Shrike Avci, Huseyin Hu, Ning Kim, Duckjin Branco, Cristina Aleman, Julio Massa, Solange Silvestri, Antonia Kang, Jian Desalvo, Anna Hussaini, Mohammed Abdullah Chae, Su‐Kyoung Polini, Alessandro Bhise, Nupura Hussain, Mohammad Asif Lee, HeaYeon Dokmeci, Mehmet R. Khademhosseini, Ali Adv Sci (Weinh) Full Papers Development of an efficient sensing platform capable of continual monitoring of biomarkers is needed to assess the functionality of the in vitro organoids and to evaluate their biological responses toward pharmaceutical compounds or chemical species over extended periods of time. Here, a novel label‐free microfluidic electrochemical (EC) biosensor with a unique built‐in on‐chip regeneration capability for continual measurement of cell‐secreted soluble biomarkers from an organoid culture in a fully automated manner without attenuating the sensor sensitivity is reported. The microfluidic EC biosensors are integrated with a human liver‐on‐a‐chip platform for continual monitoring of the metabolic activity of the organoids by measuring the levels of secreted biomarkers for up to 7 d, where the metabolic activity of the organoids is altered by a systemically applied drug. The variations in the biomarker levels are successfully measured by the microfluidic regenerative EC biosensors and agree well with cellular viability and enzyme‐linked immunosorbent assay analyses, validating the accuracy of the unique sensing platform. It is believed that this versatile and robust microfluidic EC biosensor that is capable of automated and continual detection of soluble biomarkers will find widespread use for long‐term monitoring of human organoids during drug toxicity studies or efficacy assessments of in vitro platforms. John Wiley and Sons Inc. 2017-03-06 /pmc/articles/PMC5441508/ /pubmed/28546915 http://dx.doi.org/10.1002/advs.201600522 Text en © 2017 The Authors. Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Full Papers
Shin, Su Ryon
Kilic, Tugba
Zhang, Yu Shrike
Avci, Huseyin
Hu, Ning
Kim, Duckjin
Branco, Cristina
Aleman, Julio
Massa, Solange
Silvestri, Antonia
Kang, Jian
Desalvo, Anna
Hussaini, Mohammed Abdullah
Chae, Su‐Kyoung
Polini, Alessandro
Bhise, Nupura
Hussain, Mohammad Asif
Lee, HeaYeon
Dokmeci, Mehmet R.
Khademhosseini, Ali
Label‐Free and Regenerative Electrochemical Microfluidic Biosensors for Continual Monitoring of Cell Secretomes
title Label‐Free and Regenerative Electrochemical Microfluidic Biosensors for Continual Monitoring of Cell Secretomes
title_full Label‐Free and Regenerative Electrochemical Microfluidic Biosensors for Continual Monitoring of Cell Secretomes
title_fullStr Label‐Free and Regenerative Electrochemical Microfluidic Biosensors for Continual Monitoring of Cell Secretomes
title_full_unstemmed Label‐Free and Regenerative Electrochemical Microfluidic Biosensors for Continual Monitoring of Cell Secretomes
title_short Label‐Free and Regenerative Electrochemical Microfluidic Biosensors for Continual Monitoring of Cell Secretomes
title_sort label‐free and regenerative electrochemical microfluidic biosensors for continual monitoring of cell secretomes
topic Full Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5441508/
https://www.ncbi.nlm.nih.gov/pubmed/28546915
http://dx.doi.org/10.1002/advs.201600522
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