Complementary Benzophenone Cross-Linking/Mass Spectrometry Photochemistry

[Image: see text] Use of a heterobifunctional photoactivatable cross-linker, sulfo-SDA (diazirine), has yielded high-density data that facilitated structure modeling of individual proteins. We expand the photoactivatable chemistry toolbox here with a second reagent, sulfo-SBP (benzophenone). This fu...

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Autores principales: Belsom, Adam, Mudd, Gemma, Giese, Sven, Auer, Manfred, Rappsilber, Juri
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2017
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5441754/
https://www.ncbi.nlm.nih.gov/pubmed/28430416
http://dx.doi.org/10.1021/acs.analchem.6b04938
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author Belsom, Adam
Mudd, Gemma
Giese, Sven
Auer, Manfred
Rappsilber, Juri
author_facet Belsom, Adam
Mudd, Gemma
Giese, Sven
Auer, Manfred
Rappsilber, Juri
author_sort Belsom, Adam
collection PubMed
description [Image: see text] Use of a heterobifunctional photoactivatable cross-linker, sulfo-SDA (diazirine), has yielded high-density data that facilitated structure modeling of individual proteins. We expand the photoactivatable chemistry toolbox here with a second reagent, sulfo-SBP (benzophenone). This further increases the density of photo-cross-linking to a factor of 20× over conventional cross-linking. Importantly, the two different photoactivatable groups display orthogonal directionality, enabling access to different protein regions, unreachable with a single cross-linker.
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spelling pubmed-54417542017-05-24 Complementary Benzophenone Cross-Linking/Mass Spectrometry Photochemistry Belsom, Adam Mudd, Gemma Giese, Sven Auer, Manfred Rappsilber, Juri Anal Chem [Image: see text] Use of a heterobifunctional photoactivatable cross-linker, sulfo-SDA (diazirine), has yielded high-density data that facilitated structure modeling of individual proteins. We expand the photoactivatable chemistry toolbox here with a second reagent, sulfo-SBP (benzophenone). This further increases the density of photo-cross-linking to a factor of 20× over conventional cross-linking. Importantly, the two different photoactivatable groups display orthogonal directionality, enabling access to different protein regions, unreachable with a single cross-linker. American Chemical Society 2017-04-21 2017-05-16 /pmc/articles/PMC5441754/ /pubmed/28430416 http://dx.doi.org/10.1021/acs.analchem.6b04938 Text en Copyright © 2017 American Chemical Society This is an open access article published under a Creative Commons Attribution (CC-BY) License (http://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html) , which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.
spellingShingle Belsom, Adam
Mudd, Gemma
Giese, Sven
Auer, Manfred
Rappsilber, Juri
Complementary Benzophenone Cross-Linking/Mass Spectrometry Photochemistry
title Complementary Benzophenone Cross-Linking/Mass Spectrometry Photochemistry
title_full Complementary Benzophenone Cross-Linking/Mass Spectrometry Photochemistry
title_fullStr Complementary Benzophenone Cross-Linking/Mass Spectrometry Photochemistry
title_full_unstemmed Complementary Benzophenone Cross-Linking/Mass Spectrometry Photochemistry
title_short Complementary Benzophenone Cross-Linking/Mass Spectrometry Photochemistry
title_sort complementary benzophenone cross-linking/mass spectrometry photochemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5441754/
https://www.ncbi.nlm.nih.gov/pubmed/28430416
http://dx.doi.org/10.1021/acs.analchem.6b04938
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AT muddgemma complementarybenzophenonecrosslinkingmassspectrometryphotochemistry
AT giesesven complementarybenzophenonecrosslinkingmassspectrometryphotochemistry
AT auermanfred complementarybenzophenonecrosslinkingmassspectrometryphotochemistry
AT rappsilberjuri complementarybenzophenonecrosslinkingmassspectrometryphotochemistry

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