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SPL33, encoding an eEF1A-like protein, negatively regulates cell death and defense responses in rice

Lesion-mimic mutants are useful to dissect programmed cell death and defense-related pathways in plants. Here we identified a new rice lesion-mimic mutant, spotted leaf 33 (spl33) and cloned the causal gene by a map-based cloning strategy. SPL33 encodes a eukaryotic translation elongation factor 1 a...

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Autores principales: Wang, Shuai, Lei, Cailin, Wang, Jiulin, Ma, Jian, Tang, Sha, Wang, Chunlian, Zhao, Kaijun, Tian, Peng, Zhang, Huan, Qi, Changyan, Cheng, Zhijun, Zhang, Xin, Guo, Xiuping, Liu, Linglong, Wu, Chuanyin, Wan, Jianmin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5441852/
https://www.ncbi.nlm.nih.gov/pubmed/28199670
http://dx.doi.org/10.1093/jxb/erx001
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author Wang, Shuai
Lei, Cailin
Wang, Jiulin
Ma, Jian
Tang, Sha
Wang, Chunlian
Zhao, Kaijun
Tian, Peng
Zhang, Huan
Qi, Changyan
Cheng, Zhijun
Zhang, Xin
Guo, Xiuping
Liu, Linglong
Wu, Chuanyin
Wan, Jianmin
author_facet Wang, Shuai
Lei, Cailin
Wang, Jiulin
Ma, Jian
Tang, Sha
Wang, Chunlian
Zhao, Kaijun
Tian, Peng
Zhang, Huan
Qi, Changyan
Cheng, Zhijun
Zhang, Xin
Guo, Xiuping
Liu, Linglong
Wu, Chuanyin
Wan, Jianmin
author_sort Wang, Shuai
collection PubMed
description Lesion-mimic mutants are useful to dissect programmed cell death and defense-related pathways in plants. Here we identified a new rice lesion-mimic mutant, spotted leaf 33 (spl33) and cloned the causal gene by a map-based cloning strategy. SPL33 encodes a eukaryotic translation elongation factor 1 alpha (eEF1A)-like protein consisting of a non-functional zinc finger domain and three functional EF-Tu domains. spl33 exhibited programmed cell death-mediated cell death and early leaf senescence, as evidenced by analyses of four histochemical markers, namely H(2)O(2) accumulation, cell death, callose accumulation and TUNEL-positive nuclei, and by four indicators, namely loss of chlorophyll, breakdown of chloroplasts, down-regulation of photosynthesis-related genes, and up-regulation of senescence-associated genes. Defense responses were induced in the spl33 mutant, as shown by enhanced resistance to both the fungal pathogen Magnaporthe oryzae and the bacterial pathogen Xanthomonas oryzae pv. oryzae and by up-regulation of defense response genes. Transcriptome analysis of the spl33 mutant and its wild type provided further evidence for the biological effects of loss of SPL33 function in cell death, leaf senescence and defense responses in rice. Detailed analyses showed that reactive oxygen species accumulation may be the cause of cell death in the spl33 mutant, whereas uncontrolled activation of multiple innate immunity-related receptor genes and signaling molecules may be responsible for the enhanced disease resistance observed in spl33. Thus, we have demonstrated involvement of an eEF1A-like protein in programmed cell death and provided a link to defense responses in rice.
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spelling pubmed-54418522017-05-30 SPL33, encoding an eEF1A-like protein, negatively regulates cell death and defense responses in rice Wang, Shuai Lei, Cailin Wang, Jiulin Ma, Jian Tang, Sha Wang, Chunlian Zhao, Kaijun Tian, Peng Zhang, Huan Qi, Changyan Cheng, Zhijun Zhang, Xin Guo, Xiuping Liu, Linglong Wu, Chuanyin Wan, Jianmin J Exp Bot Research Paper Lesion-mimic mutants are useful to dissect programmed cell death and defense-related pathways in plants. Here we identified a new rice lesion-mimic mutant, spotted leaf 33 (spl33) and cloned the causal gene by a map-based cloning strategy. SPL33 encodes a eukaryotic translation elongation factor 1 alpha (eEF1A)-like protein consisting of a non-functional zinc finger domain and three functional EF-Tu domains. spl33 exhibited programmed cell death-mediated cell death and early leaf senescence, as evidenced by analyses of four histochemical markers, namely H(2)O(2) accumulation, cell death, callose accumulation and TUNEL-positive nuclei, and by four indicators, namely loss of chlorophyll, breakdown of chloroplasts, down-regulation of photosynthesis-related genes, and up-regulation of senescence-associated genes. Defense responses were induced in the spl33 mutant, as shown by enhanced resistance to both the fungal pathogen Magnaporthe oryzae and the bacterial pathogen Xanthomonas oryzae pv. oryzae and by up-regulation of defense response genes. Transcriptome analysis of the spl33 mutant and its wild type provided further evidence for the biological effects of loss of SPL33 function in cell death, leaf senescence and defense responses in rice. Detailed analyses showed that reactive oxygen species accumulation may be the cause of cell death in the spl33 mutant, whereas uncontrolled activation of multiple innate immunity-related receptor genes and signaling molecules may be responsible for the enhanced disease resistance observed in spl33. Thus, we have demonstrated involvement of an eEF1A-like protein in programmed cell death and provided a link to defense responses in rice. Oxford University Press 2017-02-15 2017-02-11 /pmc/articles/PMC5441852/ /pubmed/28199670 http://dx.doi.org/10.1093/jxb/erx001 Text en © The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Wang, Shuai
Lei, Cailin
Wang, Jiulin
Ma, Jian
Tang, Sha
Wang, Chunlian
Zhao, Kaijun
Tian, Peng
Zhang, Huan
Qi, Changyan
Cheng, Zhijun
Zhang, Xin
Guo, Xiuping
Liu, Linglong
Wu, Chuanyin
Wan, Jianmin
SPL33, encoding an eEF1A-like protein, negatively regulates cell death and defense responses in rice
title SPL33, encoding an eEF1A-like protein, negatively regulates cell death and defense responses in rice
title_full SPL33, encoding an eEF1A-like protein, negatively regulates cell death and defense responses in rice
title_fullStr SPL33, encoding an eEF1A-like protein, negatively regulates cell death and defense responses in rice
title_full_unstemmed SPL33, encoding an eEF1A-like protein, negatively regulates cell death and defense responses in rice
title_short SPL33, encoding an eEF1A-like protein, negatively regulates cell death and defense responses in rice
title_sort spl33, encoding an eef1a-like protein, negatively regulates cell death and defense responses in rice
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5441852/
https://www.ncbi.nlm.nih.gov/pubmed/28199670
http://dx.doi.org/10.1093/jxb/erx001
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