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Tumor-associated autoantibodies are useful biomarkers in immunodiagnosis of α-fetoprotein-negative hepatocellular carcinoma

AIM: To determine the prevalence and diagnostic value of autoantibodies in α-fetoprotein (AFP)-negative hepatocellular carcinoma (HCC). METHODS: Fifty-six serum samples from AFP-negative HCC cases, 86 from AFP-positive HCC cases, 168 from chronic liver disease cases, and 59 from normal human control...

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Autores principales: Wang, Ting, Liu, Mei, Zheng, Su-Jun, Bian, Dan-Dan, Zhang, Jin-Yan, Yao, Jia, Zheng, Qing-Fen, Shi, A-Meng, Li, Wen-Han, Li, Lu, Chen, Yu, Wang, Jin-Hai, Duan, Zhong-Ping, Dong, Lei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Baishideng Publishing Group Inc 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5442085/
https://www.ncbi.nlm.nih.gov/pubmed/28596685
http://dx.doi.org/10.3748/wjg.v23.i19.3496
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author Wang, Ting
Liu, Mei
Zheng, Su-Jun
Bian, Dan-Dan
Zhang, Jin-Yan
Yao, Jia
Zheng, Qing-Fen
Shi, A-Meng
Li, Wen-Han
Li, Lu
Chen, Yu
Wang, Jin-Hai
Duan, Zhong-Ping
Dong, Lei
author_facet Wang, Ting
Liu, Mei
Zheng, Su-Jun
Bian, Dan-Dan
Zhang, Jin-Yan
Yao, Jia
Zheng, Qing-Fen
Shi, A-Meng
Li, Wen-Han
Li, Lu
Chen, Yu
Wang, Jin-Hai
Duan, Zhong-Ping
Dong, Lei
author_sort Wang, Ting
collection PubMed
description AIM: To determine the prevalence and diagnostic value of autoantibodies in α-fetoprotein (AFP)-negative hepatocellular carcinoma (HCC). METHODS: Fifty-six serum samples from AFP-negative HCC cases, 86 from AFP-positive HCC cases, 168 from chronic liver disease cases, and 59 from normal human controls were included in this study. Autoantibodies to nucleophosmin (NPM)1, 14-3-3zeta and mouse double minute 2 homolog (MDM2) proteins in AFP-negative HCC serum were evaluated by enzyme-linked immunosorbent assay. Partially positive sera were further evaluated by western blotting. Immunohistochemistry was used to detect the expression of three tumor-associated antigens (TAAs) in AFP-negative HCC and normal control tissues. RESULTS: The frequency of autoantibodies to the three TAAs in AFP-negative HCC sera was 21.4%, 19.6% and 19.6%, which was significantly higher than in the chronic liver disease cases and normal human controls (P < 0.01) as well as AFP-positive HCC cases. The sensitivity of the three autoantibodies for diagnosis of AFP-negative HCC ranged from 19.6% to 21.4%, and the specificity was approximately 95%. When the three autoantibodies were combined, the sensitivity reached 30.4% and the specificity reached 91.6%. CONCLUSION: Autoantibodies to NPM1, 14-3-3zeta and MDM2 may be useful biomarkers for immunodiagnosis of AFP-negative HCC.
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spelling pubmed-54420852017-06-08 Tumor-associated autoantibodies are useful biomarkers in immunodiagnosis of α-fetoprotein-negative hepatocellular carcinoma Wang, Ting Liu, Mei Zheng, Su-Jun Bian, Dan-Dan Zhang, Jin-Yan Yao, Jia Zheng, Qing-Fen Shi, A-Meng Li, Wen-Han Li, Lu Chen, Yu Wang, Jin-Hai Duan, Zhong-Ping Dong, Lei World J Gastroenterol Retrospective Cohort Study AIM: To determine the prevalence and diagnostic value of autoantibodies in α-fetoprotein (AFP)-negative hepatocellular carcinoma (HCC). METHODS: Fifty-six serum samples from AFP-negative HCC cases, 86 from AFP-positive HCC cases, 168 from chronic liver disease cases, and 59 from normal human controls were included in this study. Autoantibodies to nucleophosmin (NPM)1, 14-3-3zeta and mouse double minute 2 homolog (MDM2) proteins in AFP-negative HCC serum were evaluated by enzyme-linked immunosorbent assay. Partially positive sera were further evaluated by western blotting. Immunohistochemistry was used to detect the expression of three tumor-associated antigens (TAAs) in AFP-negative HCC and normal control tissues. RESULTS: The frequency of autoantibodies to the three TAAs in AFP-negative HCC sera was 21.4%, 19.6% and 19.6%, which was significantly higher than in the chronic liver disease cases and normal human controls (P < 0.01) as well as AFP-positive HCC cases. The sensitivity of the three autoantibodies for diagnosis of AFP-negative HCC ranged from 19.6% to 21.4%, and the specificity was approximately 95%. When the three autoantibodies were combined, the sensitivity reached 30.4% and the specificity reached 91.6%. CONCLUSION: Autoantibodies to NPM1, 14-3-3zeta and MDM2 may be useful biomarkers for immunodiagnosis of AFP-negative HCC. Baishideng Publishing Group Inc 2017-05-21 2017-05-21 /pmc/articles/PMC5442085/ /pubmed/28596685 http://dx.doi.org/10.3748/wjg.v23.i19.3496 Text en ©The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved. http://creativecommons.org/licenses/by-nc/4.0/ This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial.
spellingShingle Retrospective Cohort Study
Wang, Ting
Liu, Mei
Zheng, Su-Jun
Bian, Dan-Dan
Zhang, Jin-Yan
Yao, Jia
Zheng, Qing-Fen
Shi, A-Meng
Li, Wen-Han
Li, Lu
Chen, Yu
Wang, Jin-Hai
Duan, Zhong-Ping
Dong, Lei
Tumor-associated autoantibodies are useful biomarkers in immunodiagnosis of α-fetoprotein-negative hepatocellular carcinoma
title Tumor-associated autoantibodies are useful biomarkers in immunodiagnosis of α-fetoprotein-negative hepatocellular carcinoma
title_full Tumor-associated autoantibodies are useful biomarkers in immunodiagnosis of α-fetoprotein-negative hepatocellular carcinoma
title_fullStr Tumor-associated autoantibodies are useful biomarkers in immunodiagnosis of α-fetoprotein-negative hepatocellular carcinoma
title_full_unstemmed Tumor-associated autoantibodies are useful biomarkers in immunodiagnosis of α-fetoprotein-negative hepatocellular carcinoma
title_short Tumor-associated autoantibodies are useful biomarkers in immunodiagnosis of α-fetoprotein-negative hepatocellular carcinoma
title_sort tumor-associated autoantibodies are useful biomarkers in immunodiagnosis of α-fetoprotein-negative hepatocellular carcinoma
topic Retrospective Cohort Study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5442085/
https://www.ncbi.nlm.nih.gov/pubmed/28596685
http://dx.doi.org/10.3748/wjg.v23.i19.3496
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