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β-catenin induces expression of prohibitin gene in acute leukemic cells

Prohibitin (PHB) is a multifunctional protein conserved in eukaryotic systems and shows various expression levels in tumor cells. However, regulation of PHB is not clearly understood. Here, we focused on the regulation of PHB expression by Wnt signaling, one of dominant regulatory signals in various...

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Autores principales: Kim, Dong Min, Jang, Hanbit, Shin, Myung Geun, Kim, Jeong-Hoon, Shin, Sang Mo, Min, Sang-Hyun, Kim, Il-Chul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5442404/
https://www.ncbi.nlm.nih.gov/pubmed/28440457
http://dx.doi.org/10.3892/or.2017.5599
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author Kim, Dong Min
Jang, Hanbit
Shin, Myung Geun
Kim, Jeong-Hoon
Shin, Sang Mo
Min, Sang-Hyun
Kim, Il-Chul
author_facet Kim, Dong Min
Jang, Hanbit
Shin, Myung Geun
Kim, Jeong-Hoon
Shin, Sang Mo
Min, Sang-Hyun
Kim, Il-Chul
author_sort Kim, Dong Min
collection PubMed
description Prohibitin (PHB) is a multifunctional protein conserved in eukaryotic systems and shows various expression levels in tumor cells. However, regulation of PHB is not clearly understood. Here, we focused on the regulation of PHB expression by Wnt signaling, one of dominant regulatory signals in various leukemic cells. High mRNA levels of PHB were found in half of clinical leukemia samples. PHB expression was increased by inhibition of the MAPK pathway and decreased by activation of EGF signal. Although cell proliferating signals downregulated the transcription of PHB, treatment with lithium chloride, an analog of the Wnt signal, induced PHB level in various cell types. We identified the TCF-4/LEF-1 binding motif, CATCTG, in the promoter region of PHB by site-directed mutagenesis and ChIP assay. This β-catenin-mediated activation of PHB expression was independent of c-MYC activation, a product of Wnt signaling. These data indicate that PHB is a direct target of β-catenin and the increased level of PHB in leukemia can be regulated by Wnt signaling.
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spelling pubmed-54424042017-05-30 β-catenin induces expression of prohibitin gene in acute leukemic cells Kim, Dong Min Jang, Hanbit Shin, Myung Geun Kim, Jeong-Hoon Shin, Sang Mo Min, Sang-Hyun Kim, Il-Chul Oncol Rep Articles Prohibitin (PHB) is a multifunctional protein conserved in eukaryotic systems and shows various expression levels in tumor cells. However, regulation of PHB is not clearly understood. Here, we focused on the regulation of PHB expression by Wnt signaling, one of dominant regulatory signals in various leukemic cells. High mRNA levels of PHB were found in half of clinical leukemia samples. PHB expression was increased by inhibition of the MAPK pathway and decreased by activation of EGF signal. Although cell proliferating signals downregulated the transcription of PHB, treatment with lithium chloride, an analog of the Wnt signal, induced PHB level in various cell types. We identified the TCF-4/LEF-1 binding motif, CATCTG, in the promoter region of PHB by site-directed mutagenesis and ChIP assay. This β-catenin-mediated activation of PHB expression was independent of c-MYC activation, a product of Wnt signaling. These data indicate that PHB is a direct target of β-catenin and the increased level of PHB in leukemia can be regulated by Wnt signaling. D.A. Spandidos 2017-06 2017-04-25 /pmc/articles/PMC5442404/ /pubmed/28440457 http://dx.doi.org/10.3892/or.2017.5599 Text en Copyright: © Kim et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Kim, Dong Min
Jang, Hanbit
Shin, Myung Geun
Kim, Jeong-Hoon
Shin, Sang Mo
Min, Sang-Hyun
Kim, Il-Chul
β-catenin induces expression of prohibitin gene in acute leukemic cells
title β-catenin induces expression of prohibitin gene in acute leukemic cells
title_full β-catenin induces expression of prohibitin gene in acute leukemic cells
title_fullStr β-catenin induces expression of prohibitin gene in acute leukemic cells
title_full_unstemmed β-catenin induces expression of prohibitin gene in acute leukemic cells
title_short β-catenin induces expression of prohibitin gene in acute leukemic cells
title_sort β-catenin induces expression of prohibitin gene in acute leukemic cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5442404/
https://www.ncbi.nlm.nih.gov/pubmed/28440457
http://dx.doi.org/10.3892/or.2017.5599
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