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Live-cell imaging to compare the transfection and gene silencing efficiency of calcium phosphate nanoparticles and a liposomal transfection agent
The processing of DNA (for transfection) and short interfering RNA (siRNA; for gene silencing), introduced into HeLa cells by triple-shell calcium phosphate nanoparticles, was followed by live-cell imaging. For comparison, the commercial liposomal transfection agent Lipofectamine was used. The cells...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5442419/ https://www.ncbi.nlm.nih.gov/pubmed/28218744 http://dx.doi.org/10.1038/gt.2017.13 |