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Peptidoglycan Association of Murein Lipoprotein Is Required for KpsD-Dependent Group 2 Capsular Polysaccharide Expression and Serum Resistance in a Uropathogenic Escherichia coli Isolate
Murein lipoprotein (Lpp) and peptidoglycan-associated lipoprotein (Pal) are major outer membrane lipoproteins in Escherichia coli. Their roles in cell-envelope integrity have been documented in E. coli laboratory strains, and while Lpp has been linked to serum resistance in vitro, the underlying mec...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5442458/ https://www.ncbi.nlm.nih.gov/pubmed/28536290 http://dx.doi.org/10.1128/mBio.00603-17 |
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author | Diao, Jingyu Bouwman, Catrien Yan, Donghong Kang, Jing Katakam, Anand K. Liu, Peter Pantua, Homer Abbas, Alexander R. Nickerson, Nicholas N. Austin, Cary Reichelt, Mike Sandoval, Wendy Xu, Min Whitfield, Chris Kapadia, Sharookh B. |
author_facet | Diao, Jingyu Bouwman, Catrien Yan, Donghong Kang, Jing Katakam, Anand K. Liu, Peter Pantua, Homer Abbas, Alexander R. Nickerson, Nicholas N. Austin, Cary Reichelt, Mike Sandoval, Wendy Xu, Min Whitfield, Chris Kapadia, Sharookh B. |
author_sort | Diao, Jingyu |
collection | PubMed |
description | Murein lipoprotein (Lpp) and peptidoglycan-associated lipoprotein (Pal) are major outer membrane lipoproteins in Escherichia coli. Their roles in cell-envelope integrity have been documented in E. coli laboratory strains, and while Lpp has been linked to serum resistance in vitro, the underlying mechanism has not been established. Here, lpp and pal mutants of uropathogenic E. coli strain CFT073 showed reduced survival in a mouse bacteremia model, but only the lpp mutant was sensitive to serum killing in vitro. The peptidoglycan-bound Lpp form was specifically required for preventing complement-mediated bacterial lysis in vitro and complement-mediated clearance in vivo. Compared to the wild-type strain, the lpp mutant had impaired K2 capsular polysaccharide production and was unable to respond to exposure to serum by elevating capsular polysaccharide amounts. These properties correlated with altered cellular distribution of KpsD, the predicted outer membrane translocon for “group 2” capsular polysaccharides. We identified a novel Lpp-dependent association between functional KpsD and peptidoglycan, highlighting important interplay between cell envelope components required for resistance to complement-mediated lysis in uropathogenic E. coli isolates. |
format | Online Article Text |
id | pubmed-5442458 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | American Society for Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-54424582017-06-01 Peptidoglycan Association of Murein Lipoprotein Is Required for KpsD-Dependent Group 2 Capsular Polysaccharide Expression and Serum Resistance in a Uropathogenic Escherichia coli Isolate Diao, Jingyu Bouwman, Catrien Yan, Donghong Kang, Jing Katakam, Anand K. Liu, Peter Pantua, Homer Abbas, Alexander R. Nickerson, Nicholas N. Austin, Cary Reichelt, Mike Sandoval, Wendy Xu, Min Whitfield, Chris Kapadia, Sharookh B. mBio Research Article Murein lipoprotein (Lpp) and peptidoglycan-associated lipoprotein (Pal) are major outer membrane lipoproteins in Escherichia coli. Their roles in cell-envelope integrity have been documented in E. coli laboratory strains, and while Lpp has been linked to serum resistance in vitro, the underlying mechanism has not been established. Here, lpp and pal mutants of uropathogenic E. coli strain CFT073 showed reduced survival in a mouse bacteremia model, but only the lpp mutant was sensitive to serum killing in vitro. The peptidoglycan-bound Lpp form was specifically required for preventing complement-mediated bacterial lysis in vitro and complement-mediated clearance in vivo. Compared to the wild-type strain, the lpp mutant had impaired K2 capsular polysaccharide production and was unable to respond to exposure to serum by elevating capsular polysaccharide amounts. These properties correlated with altered cellular distribution of KpsD, the predicted outer membrane translocon for “group 2” capsular polysaccharides. We identified a novel Lpp-dependent association between functional KpsD and peptidoglycan, highlighting important interplay between cell envelope components required for resistance to complement-mediated lysis in uropathogenic E. coli isolates. American Society for Microbiology 2017-05-23 /pmc/articles/PMC5442458/ /pubmed/28536290 http://dx.doi.org/10.1128/mBio.00603-17 Text en Copyright © 2017 Diao et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (http://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Article Diao, Jingyu Bouwman, Catrien Yan, Donghong Kang, Jing Katakam, Anand K. Liu, Peter Pantua, Homer Abbas, Alexander R. Nickerson, Nicholas N. Austin, Cary Reichelt, Mike Sandoval, Wendy Xu, Min Whitfield, Chris Kapadia, Sharookh B. Peptidoglycan Association of Murein Lipoprotein Is Required for KpsD-Dependent Group 2 Capsular Polysaccharide Expression and Serum Resistance in a Uropathogenic Escherichia coli Isolate |
title | Peptidoglycan Association of Murein Lipoprotein Is Required for KpsD-Dependent Group 2 Capsular Polysaccharide Expression and Serum Resistance in a Uropathogenic Escherichia coli Isolate |
title_full | Peptidoglycan Association of Murein Lipoprotein Is Required for KpsD-Dependent Group 2 Capsular Polysaccharide Expression and Serum Resistance in a Uropathogenic Escherichia coli Isolate |
title_fullStr | Peptidoglycan Association of Murein Lipoprotein Is Required for KpsD-Dependent Group 2 Capsular Polysaccharide Expression and Serum Resistance in a Uropathogenic Escherichia coli Isolate |
title_full_unstemmed | Peptidoglycan Association of Murein Lipoprotein Is Required for KpsD-Dependent Group 2 Capsular Polysaccharide Expression and Serum Resistance in a Uropathogenic Escherichia coli Isolate |
title_short | Peptidoglycan Association of Murein Lipoprotein Is Required for KpsD-Dependent Group 2 Capsular Polysaccharide Expression and Serum Resistance in a Uropathogenic Escherichia coli Isolate |
title_sort | peptidoglycan association of murein lipoprotein is required for kpsd-dependent group 2 capsular polysaccharide expression and serum resistance in a uropathogenic escherichia coli isolate |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5442458/ https://www.ncbi.nlm.nih.gov/pubmed/28536290 http://dx.doi.org/10.1128/mBio.00603-17 |
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