Selective Expansion of Skeletal Muscle Stem Cells from Bulk Muscle Cells in Soft Three‐Dimensional Fibrin Gel

Muscle stem cells (MuSCs) exhibit robust myogenic potential in vivo, thus providing a promising curative treatment for muscle disorders. Ex vivo expansion of adult MuSCs is highly desired to achieve a therapeutic cell dose because of their scarcity in limited muscle biopsies. Sorting of pure MuSCs i...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhu, Pei, Zhou, Yalu, Wu, Furen, Hong, Yuanfan, Wang, Xin, Shekhawat, Gajendra, Mosenson, Jeffrey, Wu, Wen‐Shu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Translational Research Articles and Reviews
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5442710/
https://www.ncbi.nlm.nih.gov/pubmed/28244269
http://dx.doi.org/10.1002/sctm.16-0427
_version_ 1783238450268340224
author Zhu, Pei
Zhou, Yalu
Wu, Furen
Hong, Yuanfan
Wang, Xin
Shekhawat, Gajendra
Mosenson, Jeffrey
Wu, Wen‐Shu
author_facet Zhu, Pei
Zhou, Yalu
Wu, Furen
Hong, Yuanfan
Wang, Xin
Shekhawat, Gajendra
Mosenson, Jeffrey
Wu, Wen‐Shu
author_sort Zhu, Pei
collection PubMed
description Muscle stem cells (MuSCs) exhibit robust myogenic potential in vivo, thus providing a promising curative treatment for muscle disorders. Ex vivo expansion of adult MuSCs is highly desired to achieve a therapeutic cell dose because of their scarcity in limited muscle biopsies. Sorting of pure MuSCs is generally required for all the current culture systems. Here we developed a soft three‐dimensional (3D) salmon fibrin gel culture system that can selectively expand mouse MuSCs from bulk skeletal muscle preparations without cell sorting and faithfully maintain their regenerative capacity in culture. Our study established a novel platform for convenient ex vivo expansion of MuSCs, thus greatly advancing stem cell‐based therapies for various muscle disorders. Stem Cells Translational Medicine 2017;6:1412–1423
format Online
Article
Text
id pubmed-5442710
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-54427102017-06-15 Selective Expansion of Skeletal Muscle Stem Cells from Bulk Muscle Cells in Soft Three‐Dimensional Fibrin Gel Zhu, Pei Zhou, Yalu Wu, Furen Hong, Yuanfan Wang, Xin Shekhawat, Gajendra Mosenson, Jeffrey Wu, Wen‐Shu Stem Cells Transl Med Translational Research Articles and Reviews Muscle stem cells (MuSCs) exhibit robust myogenic potential in vivo, thus providing a promising curative treatment for muscle disorders. Ex vivo expansion of adult MuSCs is highly desired to achieve a therapeutic cell dose because of their scarcity in limited muscle biopsies. Sorting of pure MuSCs is generally required for all the current culture systems. Here we developed a soft three‐dimensional (3D) salmon fibrin gel culture system that can selectively expand mouse MuSCs from bulk skeletal muscle preparations without cell sorting and faithfully maintain their regenerative capacity in culture. Our study established a novel platform for convenient ex vivo expansion of MuSCs, thus greatly advancing stem cell‐based therapies for various muscle disorders. Stem Cells Translational Medicine 2017;6:1412–1423 John Wiley and Sons Inc. 2017-02-28 2017-05 /pmc/articles/PMC5442710/ /pubmed/28244269 http://dx.doi.org/10.1002/sctm.16-0427 Text en © 2017 The Authors Stem Cells Translational Medicine published by Wiley Periodicals, Inc. on behalf of AlphaMed Press This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Translational Research Articles and Reviews
Zhu, Pei
Zhou, Yalu
Wu, Furen
Hong, Yuanfan
Wang, Xin
Shekhawat, Gajendra
Mosenson, Jeffrey
Wu, Wen‐Shu
Selective Expansion of Skeletal Muscle Stem Cells from Bulk Muscle Cells in Soft Three‐Dimensional Fibrin Gel
title Selective Expansion of Skeletal Muscle Stem Cells from Bulk Muscle Cells in Soft Three‐Dimensional Fibrin Gel
title_full Selective Expansion of Skeletal Muscle Stem Cells from Bulk Muscle Cells in Soft Three‐Dimensional Fibrin Gel
title_fullStr Selective Expansion of Skeletal Muscle Stem Cells from Bulk Muscle Cells in Soft Three‐Dimensional Fibrin Gel
title_full_unstemmed Selective Expansion of Skeletal Muscle Stem Cells from Bulk Muscle Cells in Soft Three‐Dimensional Fibrin Gel
title_short Selective Expansion of Skeletal Muscle Stem Cells from Bulk Muscle Cells in Soft Three‐Dimensional Fibrin Gel
title_sort selective expansion of skeletal muscle stem cells from bulk muscle cells in soft three‐dimensional fibrin gel
topic Translational Research Articles and Reviews
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5442710/
https://www.ncbi.nlm.nih.gov/pubmed/28244269
http://dx.doi.org/10.1002/sctm.16-0427
work_keys_str_mv AT zhupei selectiveexpansionofskeletalmusclestemcellsfrombulkmusclecellsinsoftthreedimensionalfibringel
AT zhouyalu selectiveexpansionofskeletalmusclestemcellsfrombulkmusclecellsinsoftthreedimensionalfibringel
AT wufuren selectiveexpansionofskeletalmusclestemcellsfrombulkmusclecellsinsoftthreedimensionalfibringel
AT hongyuanfan selectiveexpansionofskeletalmusclestemcellsfrombulkmusclecellsinsoftthreedimensionalfibringel
AT wangxin selectiveexpansionofskeletalmusclestemcellsfrombulkmusclecellsinsoftthreedimensionalfibringel
AT shekhawatgajendra selectiveexpansionofskeletalmusclestemcellsfrombulkmusclecellsinsoftthreedimensionalfibringel
AT mosensonjeffrey selectiveexpansionofskeletalmusclestemcellsfrombulkmusclecellsinsoftthreedimensionalfibringel
AT wuwenshu selectiveexpansionofskeletalmusclestemcellsfrombulkmusclecellsinsoftthreedimensionalfibringel

Ejemplares similares