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A multi-head intradermal electroporation device allows for tailored and increased dose DNA vaccine delivery to the skin
The identification of an effective and tolerable delivery method is a necessity for the success of DNA vaccines in the clinic. This manuscript describes the development and validation of a multi-headed intradermal electroporation device which would be applicable for delivering multiple DNA vaccine p...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5443063/ https://www.ncbi.nlm.nih.gov/pubmed/25483486 http://dx.doi.org/10.4161/hv.29671 |
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author | McCoy, Jay R Mendoza, Janess M Spik, Kristin W Badger, Catherine Gomez, Alan F Schmaljohn, Connie S Sardesai, Niranjan Y Broderick, Kate E |
author_facet | McCoy, Jay R Mendoza, Janess M Spik, Kristin W Badger, Catherine Gomez, Alan F Schmaljohn, Connie S Sardesai, Niranjan Y Broderick, Kate E |
author_sort | McCoy, Jay R |
collection | PubMed |
description | The identification of an effective and tolerable delivery method is a necessity for the success of DNA vaccines in the clinic. This manuscript describes the development and validation of a multi-headed intradermal electroporation device which would be applicable for delivering multiple DNA vaccine plasmids simultaneously but spatially separated. Reporter gene plasmids expressing green and red fluorescent proteins were used to demonstrate the impact of spatial separation on DNA delivery to increase the number of transfected cells and avoid interference through visible expression patterns. To investigate the impact of plasmid interference on immunogenicity, a disease target was investigated where issues with multi-valent vaccines had been previously described. DNA-based Hantaan and Puumala virus vaccines were delivered separately or as a combination and the effect of multi-valence was determined by appropriate assays. While a negative impact was observed for both antigenic vaccines when delivered together, these effects were mitigated when the vaccine was delivered using the multi-head device. We also demonstrate how the multi-head device facilitates higher dose delivery to the skin resulting in improved immune responses. This new multi-head platform device is an efficient, tolerable and non-invasive method to deliver multiple plasmid DNA constructs simultaneously allowing the tailoring of delivery sites for combination vaccines. Additionally, this device would allow the delivery of multi-plasmid vaccine formulations without risk of impacted immune responses through interference. Such a low-cost, easy to use device platform for the delivery of multi-agent DNA vaccines would have direct applications by the military and healthcare sectors for mass vaccination purposes. |
format | Online Article Text |
id | pubmed-5443063 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-54430632017-06-01 A multi-head intradermal electroporation device allows for tailored and increased dose DNA vaccine delivery to the skin McCoy, Jay R Mendoza, Janess M Spik, Kristin W Badger, Catherine Gomez, Alan F Schmaljohn, Connie S Sardesai, Niranjan Y Broderick, Kate E Hum Vaccin Immunother Research Paper The identification of an effective and tolerable delivery method is a necessity for the success of DNA vaccines in the clinic. This manuscript describes the development and validation of a multi-headed intradermal electroporation device which would be applicable for delivering multiple DNA vaccine plasmids simultaneously but spatially separated. Reporter gene plasmids expressing green and red fluorescent proteins were used to demonstrate the impact of spatial separation on DNA delivery to increase the number of transfected cells and avoid interference through visible expression patterns. To investigate the impact of plasmid interference on immunogenicity, a disease target was investigated where issues with multi-valent vaccines had been previously described. DNA-based Hantaan and Puumala virus vaccines were delivered separately or as a combination and the effect of multi-valence was determined by appropriate assays. While a negative impact was observed for both antigenic vaccines when delivered together, these effects were mitigated when the vaccine was delivered using the multi-head device. We also demonstrate how the multi-head device facilitates higher dose delivery to the skin resulting in improved immune responses. This new multi-head platform device is an efficient, tolerable and non-invasive method to deliver multiple plasmid DNA constructs simultaneously allowing the tailoring of delivery sites for combination vaccines. Additionally, this device would allow the delivery of multi-plasmid vaccine formulations without risk of impacted immune responses through interference. Such a low-cost, easy to use device platform for the delivery of multi-agent DNA vaccines would have direct applications by the military and healthcare sectors for mass vaccination purposes. Taylor & Francis 2014-11-21 /pmc/articles/PMC5443063/ /pubmed/25483486 http://dx.doi.org/10.4161/hv.29671 Text en © 2014 The Author(s). Published with license by Taylor & Francis Group, LLC http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted. |
spellingShingle | Research Paper McCoy, Jay R Mendoza, Janess M Spik, Kristin W Badger, Catherine Gomez, Alan F Schmaljohn, Connie S Sardesai, Niranjan Y Broderick, Kate E A multi-head intradermal electroporation device allows for tailored and increased dose DNA vaccine delivery to the skin |
title | A multi-head intradermal electroporation device allows for tailored and increased dose DNA vaccine delivery to the skin |
title_full | A multi-head intradermal electroporation device allows for tailored and increased dose DNA vaccine delivery to the skin |
title_fullStr | A multi-head intradermal electroporation device allows for tailored and increased dose DNA vaccine delivery to the skin |
title_full_unstemmed | A multi-head intradermal electroporation device allows for tailored and increased dose DNA vaccine delivery to the skin |
title_short | A multi-head intradermal electroporation device allows for tailored and increased dose DNA vaccine delivery to the skin |
title_sort | multi-head intradermal electroporation device allows for tailored and increased dose dna vaccine delivery to the skin |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5443063/ https://www.ncbi.nlm.nih.gov/pubmed/25483486 http://dx.doi.org/10.4161/hv.29671 |
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