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Schwann cells promote the capability of neural stem cells to differentiate into neurons and secret neurotrophic factors
The present study investigated whether co-culturing Schwann cells (SCs) with neural stem cells (NSCs) improves viability, direction of differentiation and secretion of brain-derived neurotrophic factor (BDNF) and glial cell-derived neurotrophic factor (GDNF) in NSCs. The three groups assessed were a...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5443174/ https://www.ncbi.nlm.nih.gov/pubmed/28565804 http://dx.doi.org/10.3892/etm.2017.4183 |
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author | Yu, Ziwei Men, Yongzhi Dong, Pin |
author_facet | Yu, Ziwei Men, Yongzhi Dong, Pin |
author_sort | Yu, Ziwei |
collection | PubMed |
description | The present study investigated whether co-culturing Schwann cells (SCs) with neural stem cells (NSCs) improves viability, direction of differentiation and secretion of brain-derived neurotrophic factor (BDNF) and glial cell-derived neurotrophic factor (GDNF) in NSCs. The three groups assessed were as follows: SCs, NSCs, and a co-culture of SCs and NSCs. Cellular morphological changes were observed under an inverted phase contrast microscope and quantified. Cells were identified by immunofluorescence staining: S100 for SCs, Nestin for NSCs, microtubule associated protein (Map) 2 and NeuN for neurons and glial fibrillary acidic protein for astrocytes. Cell viability was evaluated by MTT assay. Secretion of BDNF and GDNF was quantified; mRNA expression was quantified by reverse transcription-quantitative polymerase chain reaction. The majority of NSCs in the co-cultured group differentiated into neurons. The cell survival rate of the co-culture group was significantly higher than the other groups on days 3, 5 and 10 (P<0.01). The secretion of BDNF in the co-culture group was significantly higher than NSCs on days 3, 5 and 7 (P<0.05), while the amount of GDNF in co-culture was significantly higher than both NSCs and SCs on day 1 (P<0.05). BDNF and GDNF gene expression in the co-culture group was significantly higher than SCs (P<0.01). Gene expression of Map2 in co-culture group was also significantly higher than both NSC and SC groups (P<0.01). Therefore, co-cultured SCs and NSCs promote differentiation of NSCs into neurons and secrete higher levels of neurotropic factors including BDNF and GDNF. |
format | Online Article Text |
id | pubmed-5443174 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-54431742017-05-30 Schwann cells promote the capability of neural stem cells to differentiate into neurons and secret neurotrophic factors Yu, Ziwei Men, Yongzhi Dong, Pin Exp Ther Med Articles The present study investigated whether co-culturing Schwann cells (SCs) with neural stem cells (NSCs) improves viability, direction of differentiation and secretion of brain-derived neurotrophic factor (BDNF) and glial cell-derived neurotrophic factor (GDNF) in NSCs. The three groups assessed were as follows: SCs, NSCs, and a co-culture of SCs and NSCs. Cellular morphological changes were observed under an inverted phase contrast microscope and quantified. Cells were identified by immunofluorescence staining: S100 for SCs, Nestin for NSCs, microtubule associated protein (Map) 2 and NeuN for neurons and glial fibrillary acidic protein for astrocytes. Cell viability was evaluated by MTT assay. Secretion of BDNF and GDNF was quantified; mRNA expression was quantified by reverse transcription-quantitative polymerase chain reaction. The majority of NSCs in the co-cultured group differentiated into neurons. The cell survival rate of the co-culture group was significantly higher than the other groups on days 3, 5 and 10 (P<0.01). The secretion of BDNF in the co-culture group was significantly higher than NSCs on days 3, 5 and 7 (P<0.05), while the amount of GDNF in co-culture was significantly higher than both NSCs and SCs on day 1 (P<0.05). BDNF and GDNF gene expression in the co-culture group was significantly higher than SCs (P<0.01). Gene expression of Map2 in co-culture group was also significantly higher than both NSC and SC groups (P<0.01). Therefore, co-cultured SCs and NSCs promote differentiation of NSCs into neurons and secrete higher levels of neurotropic factors including BDNF and GDNF. D.A. Spandidos 2017-05 2017-03-06 /pmc/articles/PMC5443174/ /pubmed/28565804 http://dx.doi.org/10.3892/etm.2017.4183 Text en Copyright: © Yu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Yu, Ziwei Men, Yongzhi Dong, Pin Schwann cells promote the capability of neural stem cells to differentiate into neurons and secret neurotrophic factors |
title | Schwann cells promote the capability of neural stem cells to differentiate into neurons and secret neurotrophic factors |
title_full | Schwann cells promote the capability of neural stem cells to differentiate into neurons and secret neurotrophic factors |
title_fullStr | Schwann cells promote the capability of neural stem cells to differentiate into neurons and secret neurotrophic factors |
title_full_unstemmed | Schwann cells promote the capability of neural stem cells to differentiate into neurons and secret neurotrophic factors |
title_short | Schwann cells promote the capability of neural stem cells to differentiate into neurons and secret neurotrophic factors |
title_sort | schwann cells promote the capability of neural stem cells to differentiate into neurons and secret neurotrophic factors |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5443174/ https://www.ncbi.nlm.nih.gov/pubmed/28565804 http://dx.doi.org/10.3892/etm.2017.4183 |
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