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Application of CRISPR/Cas9 in plant biology
The CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated proteins) system was first identified in bacteria and archaea and can degrade exogenous substrates. It was developed as a gene editing technology in 2013. Over the subsequent years, it has received extensive...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5443236/ https://www.ncbi.nlm.nih.gov/pubmed/28589077 http://dx.doi.org/10.1016/j.apsb.2017.01.002 |
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author | Liu, Xuan Wu, Surui Xu, Jiao Sui, Chun Wei, Jianhe |
author_facet | Liu, Xuan Wu, Surui Xu, Jiao Sui, Chun Wei, Jianhe |
author_sort | Liu, Xuan |
collection | PubMed |
description | The CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated proteins) system was first identified in bacteria and archaea and can degrade exogenous substrates. It was developed as a gene editing technology in 2013. Over the subsequent years, it has received extensive attention owing to its easy manipulation, high efficiency, and wide application in gene mutation and transcriptional regulation in mammals and plants. The process of CRISPR/Cas is optimized constantly and its application has also expanded dramatically. Therefore, CRISPR/Cas is considered a revolutionary technology in plant biology. Here, we introduce the mechanism of the type II CRISPR/Cas called CRISPR/Cas9, update its recent advances in various applications in plants, and discuss its future prospects to provide an argument for its use in the study of medicinal plants. |
format | Online Article Text |
id | pubmed-5443236 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-54432362017-06-06 Application of CRISPR/Cas9 in plant biology Liu, Xuan Wu, Surui Xu, Jiao Sui, Chun Wei, Jianhe Acta Pharm Sin B Review The CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated proteins) system was first identified in bacteria and archaea and can degrade exogenous substrates. It was developed as a gene editing technology in 2013. Over the subsequent years, it has received extensive attention owing to its easy manipulation, high efficiency, and wide application in gene mutation and transcriptional regulation in mammals and plants. The process of CRISPR/Cas is optimized constantly and its application has also expanded dramatically. Therefore, CRISPR/Cas is considered a revolutionary technology in plant biology. Here, we introduce the mechanism of the type II CRISPR/Cas called CRISPR/Cas9, update its recent advances in various applications in plants, and discuss its future prospects to provide an argument for its use in the study of medicinal plants. Elsevier 2017-05 2017-03-11 /pmc/articles/PMC5443236/ /pubmed/28589077 http://dx.doi.org/10.1016/j.apsb.2017.01.002 Text en © 2017 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Review Liu, Xuan Wu, Surui Xu, Jiao Sui, Chun Wei, Jianhe Application of CRISPR/Cas9 in plant biology |
title | Application of CRISPR/Cas9 in plant biology |
title_full | Application of CRISPR/Cas9 in plant biology |
title_fullStr | Application of CRISPR/Cas9 in plant biology |
title_full_unstemmed | Application of CRISPR/Cas9 in plant biology |
title_short | Application of CRISPR/Cas9 in plant biology |
title_sort | application of crispr/cas9 in plant biology |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5443236/ https://www.ncbi.nlm.nih.gov/pubmed/28589077 http://dx.doi.org/10.1016/j.apsb.2017.01.002 |
work_keys_str_mv | AT liuxuan applicationofcrisprcas9inplantbiology AT wusurui applicationofcrisprcas9inplantbiology AT xujiao applicationofcrisprcas9inplantbiology AT suichun applicationofcrisprcas9inplantbiology AT weijianhe applicationofcrisprcas9inplantbiology |