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Application of CRISPR/Cas9 in plant biology

The CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated proteins) system was first identified in bacteria and archaea and can degrade exogenous substrates. It was developed as a gene editing technology in 2013. Over the subsequent years, it has received extensive...

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Detalles Bibliográficos
Autores principales: Liu, Xuan, Wu, Surui, Xu, Jiao, Sui, Chun, Wei, Jianhe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5443236/
https://www.ncbi.nlm.nih.gov/pubmed/28589077
http://dx.doi.org/10.1016/j.apsb.2017.01.002
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author Liu, Xuan
Wu, Surui
Xu, Jiao
Sui, Chun
Wei, Jianhe
author_facet Liu, Xuan
Wu, Surui
Xu, Jiao
Sui, Chun
Wei, Jianhe
author_sort Liu, Xuan
collection PubMed
description The CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated proteins) system was first identified in bacteria and archaea and can degrade exogenous substrates. It was developed as a gene editing technology in 2013. Over the subsequent years, it has received extensive attention owing to its easy manipulation, high efficiency, and wide application in gene mutation and transcriptional regulation in mammals and plants. The process of CRISPR/Cas is optimized constantly and its application has also expanded dramatically. Therefore, CRISPR/Cas is considered a revolutionary technology in plant biology. Here, we introduce the mechanism of the type II CRISPR/Cas called CRISPR/Cas9, update its recent advances in various applications in plants, and discuss its future prospects to provide an argument for its use in the study of medicinal plants.
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spelling pubmed-54432362017-06-06 Application of CRISPR/Cas9 in plant biology Liu, Xuan Wu, Surui Xu, Jiao Sui, Chun Wei, Jianhe Acta Pharm Sin B Review The CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated proteins) system was first identified in bacteria and archaea and can degrade exogenous substrates. It was developed as a gene editing technology in 2013. Over the subsequent years, it has received extensive attention owing to its easy manipulation, high efficiency, and wide application in gene mutation and transcriptional regulation in mammals and plants. The process of CRISPR/Cas is optimized constantly and its application has also expanded dramatically. Therefore, CRISPR/Cas is considered a revolutionary technology in plant biology. Here, we introduce the mechanism of the type II CRISPR/Cas called CRISPR/Cas9, update its recent advances in various applications in plants, and discuss its future prospects to provide an argument for its use in the study of medicinal plants. Elsevier 2017-05 2017-03-11 /pmc/articles/PMC5443236/ /pubmed/28589077 http://dx.doi.org/10.1016/j.apsb.2017.01.002 Text en © 2017 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Review
Liu, Xuan
Wu, Surui
Xu, Jiao
Sui, Chun
Wei, Jianhe
Application of CRISPR/Cas9 in plant biology
title Application of CRISPR/Cas9 in plant biology
title_full Application of CRISPR/Cas9 in plant biology
title_fullStr Application of CRISPR/Cas9 in plant biology
title_full_unstemmed Application of CRISPR/Cas9 in plant biology
title_short Application of CRISPR/Cas9 in plant biology
title_sort application of crispr/cas9 in plant biology
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5443236/
https://www.ncbi.nlm.nih.gov/pubmed/28589077
http://dx.doi.org/10.1016/j.apsb.2017.01.002
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