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Proteomic analysis in the Dufour’s gland of Africanized Apis mellifera workers (Hymenoptera: Apidae)

The colony of eusocial bee Apis mellifera has a reproductive queen and sterile workers performing tasks such as brood care and foraging. Chemical communication plays a crucial role in the maintenance of sociability in bees with many compounds released by the exocrine glands. The Dufour’s gland is a...

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Autores principales: Teixeira, Aparecida das Dores, Games, Patricia D., Katz, Benjamin B., Tomich, John M., Zanuncio, José C., Serrão, José Eduardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5443511/
https://www.ncbi.nlm.nih.gov/pubmed/28542566
http://dx.doi.org/10.1371/journal.pone.0177415
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author Teixeira, Aparecida das Dores
Games, Patricia D.
Katz, Benjamin B.
Tomich, John M.
Zanuncio, José C.
Serrão, José Eduardo
author_facet Teixeira, Aparecida das Dores
Games, Patricia D.
Katz, Benjamin B.
Tomich, John M.
Zanuncio, José C.
Serrão, José Eduardo
author_sort Teixeira, Aparecida das Dores
collection PubMed
description The colony of eusocial bee Apis mellifera has a reproductive queen and sterile workers performing tasks such as brood care and foraging. Chemical communication plays a crucial role in the maintenance of sociability in bees with many compounds released by the exocrine glands. The Dufour’s gland is a non-paired gland associated with the sting apparatus with important functions in the communication between members of the colony, releasing volatile chemicals that influence workers roles and tasks. However, the protein content in this gland is not well studied. This study identified differentially expressed proteins in the Dufour’s glands of nurse and forager workers of A. mellifera through 2D-gel electrophoresis and mass spectrometry. A total of 131 spots showed different expression between nurse and forager bees, and 28 proteins were identified. The identified proteins were categorized into different functions groups including protein, carbohydrate, energy and lipid metabolisms, cytoskeleton-associated proteins, detoxification, homeostasis, cell communication, constitutive and allergen. This study provides new insights of the protein content in the Dufour’s gland contributing to a more complete understanding of the biological functions of this gland in honeybees.
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spelling pubmed-54435112017-06-06 Proteomic analysis in the Dufour’s gland of Africanized Apis mellifera workers (Hymenoptera: Apidae) Teixeira, Aparecida das Dores Games, Patricia D. Katz, Benjamin B. Tomich, John M. Zanuncio, José C. Serrão, José Eduardo PLoS One Research Article The colony of eusocial bee Apis mellifera has a reproductive queen and sterile workers performing tasks such as brood care and foraging. Chemical communication plays a crucial role in the maintenance of sociability in bees with many compounds released by the exocrine glands. The Dufour’s gland is a non-paired gland associated with the sting apparatus with important functions in the communication between members of the colony, releasing volatile chemicals that influence workers roles and tasks. However, the protein content in this gland is not well studied. This study identified differentially expressed proteins in the Dufour’s glands of nurse and forager workers of A. mellifera through 2D-gel electrophoresis and mass spectrometry. A total of 131 spots showed different expression between nurse and forager bees, and 28 proteins were identified. The identified proteins were categorized into different functions groups including protein, carbohydrate, energy and lipid metabolisms, cytoskeleton-associated proteins, detoxification, homeostasis, cell communication, constitutive and allergen. This study provides new insights of the protein content in the Dufour’s gland contributing to a more complete understanding of the biological functions of this gland in honeybees. Public Library of Science 2017-05-24 /pmc/articles/PMC5443511/ /pubmed/28542566 http://dx.doi.org/10.1371/journal.pone.0177415 Text en © 2017 Teixeira et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Teixeira, Aparecida das Dores
Games, Patricia D.
Katz, Benjamin B.
Tomich, John M.
Zanuncio, José C.
Serrão, José Eduardo
Proteomic analysis in the Dufour’s gland of Africanized Apis mellifera workers (Hymenoptera: Apidae)
title Proteomic analysis in the Dufour’s gland of Africanized Apis mellifera workers (Hymenoptera: Apidae)
title_full Proteomic analysis in the Dufour’s gland of Africanized Apis mellifera workers (Hymenoptera: Apidae)
title_fullStr Proteomic analysis in the Dufour’s gland of Africanized Apis mellifera workers (Hymenoptera: Apidae)
title_full_unstemmed Proteomic analysis in the Dufour’s gland of Africanized Apis mellifera workers (Hymenoptera: Apidae)
title_short Proteomic analysis in the Dufour’s gland of Africanized Apis mellifera workers (Hymenoptera: Apidae)
title_sort proteomic analysis in the dufour’s gland of africanized apis mellifera workers (hymenoptera: apidae)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5443511/
https://www.ncbi.nlm.nih.gov/pubmed/28542566
http://dx.doi.org/10.1371/journal.pone.0177415
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