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Environmental persistence of porcine coronaviruses in feed and feed ingredients

Porcine Epidemic Diarrhea Virus (PEDV), Porcine Delta Corona Virus (PDCoV), and Transmissible Gastroenteritis Virus (TGEV) are major threats to swine health and contaminated feed plays a role in virus transmission. The objective of our study was to characterize inactivation of PEDV, PDCoV, and TGEV...

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Autores principales: Trudeau, Michaela P., Verma, Harsha, Sampedro, Fernando, Urriola, Pedro E., Shurson, Gerald C., Goyal, Sagar M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5443540/
https://www.ncbi.nlm.nih.gov/pubmed/28542235
http://dx.doi.org/10.1371/journal.pone.0178094
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author Trudeau, Michaela P.
Verma, Harsha
Sampedro, Fernando
Urriola, Pedro E.
Shurson, Gerald C.
Goyal, Sagar M.
author_facet Trudeau, Michaela P.
Verma, Harsha
Sampedro, Fernando
Urriola, Pedro E.
Shurson, Gerald C.
Goyal, Sagar M.
author_sort Trudeau, Michaela P.
collection PubMed
description Porcine Epidemic Diarrhea Virus (PEDV), Porcine Delta Corona Virus (PDCoV), and Transmissible Gastroenteritis Virus (TGEV) are major threats to swine health and contaminated feed plays a role in virus transmission. The objective of our study was to characterize inactivation of PEDV, PDCoV, and TGEV in various feed ingredient matrices. Samples of complete feed, spray dried porcine plasma, meat meal, meat and bone meal, blood meal, corn, soybean meal, and corn dried distillers grains with solubles were weighed (5 g/sample) into scintillation vials and inoculated with 1 mL of PEDV, PDCoV, or TGEV. Samples were incubated at room temperature for up to 56 days. Aliquots were removed at various time points followed by preparing serial 10-fold dilutions and inoculating in cell cultures to determine the amount of surviving virus. Inactivation kinetics were determined using the Weibull model, which estimates a delta value indicating the time necessary to reduce virus concentration by 1 log. Delta values of various ingredients were compared and analyzed as to their nutrient composition. Soybean meal had the greatest delta value (7.50 days) for PEDV (P < 0.06) as compared with all other ingredients. High delta values (P < 0.001) were observed in soybean meal for PDCoV (42.04 days) and TGEV (42.00 days). There was a moderate correlation between moisture content and the delta value for PDCoV (r = 0.49, P = 0.01) and TGEV (r = 0.41, P = 0.02). There was also a moderate negative correlation between TGEV survival and ether extract content (r = -0.51, P = 0.01). In conclusion, these results indicate that the first log reduction of PDCoV and TGEV takes the greatest amount of time in soybean meal. In addition to this, moisture and ether content appear to be an important determinant of virus survival in feed ingredients.
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spelling pubmed-54435402017-06-06 Environmental persistence of porcine coronaviruses in feed and feed ingredients Trudeau, Michaela P. Verma, Harsha Sampedro, Fernando Urriola, Pedro E. Shurson, Gerald C. Goyal, Sagar M. PLoS One Research Article Porcine Epidemic Diarrhea Virus (PEDV), Porcine Delta Corona Virus (PDCoV), and Transmissible Gastroenteritis Virus (TGEV) are major threats to swine health and contaminated feed plays a role in virus transmission. The objective of our study was to characterize inactivation of PEDV, PDCoV, and TGEV in various feed ingredient matrices. Samples of complete feed, spray dried porcine plasma, meat meal, meat and bone meal, blood meal, corn, soybean meal, and corn dried distillers grains with solubles were weighed (5 g/sample) into scintillation vials and inoculated with 1 mL of PEDV, PDCoV, or TGEV. Samples were incubated at room temperature for up to 56 days. Aliquots were removed at various time points followed by preparing serial 10-fold dilutions and inoculating in cell cultures to determine the amount of surviving virus. Inactivation kinetics were determined using the Weibull model, which estimates a delta value indicating the time necessary to reduce virus concentration by 1 log. Delta values of various ingredients were compared and analyzed as to their nutrient composition. Soybean meal had the greatest delta value (7.50 days) for PEDV (P < 0.06) as compared with all other ingredients. High delta values (P < 0.001) were observed in soybean meal for PDCoV (42.04 days) and TGEV (42.00 days). There was a moderate correlation between moisture content and the delta value for PDCoV (r = 0.49, P = 0.01) and TGEV (r = 0.41, P = 0.02). There was also a moderate negative correlation between TGEV survival and ether extract content (r = -0.51, P = 0.01). In conclusion, these results indicate that the first log reduction of PDCoV and TGEV takes the greatest amount of time in soybean meal. In addition to this, moisture and ether content appear to be an important determinant of virus survival in feed ingredients. Public Library of Science 2017-05-24 /pmc/articles/PMC5443540/ /pubmed/28542235 http://dx.doi.org/10.1371/journal.pone.0178094 Text en © 2017 Trudeau et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Trudeau, Michaela P.
Verma, Harsha
Sampedro, Fernando
Urriola, Pedro E.
Shurson, Gerald C.
Goyal, Sagar M.
Environmental persistence of porcine coronaviruses in feed and feed ingredients
title Environmental persistence of porcine coronaviruses in feed and feed ingredients
title_full Environmental persistence of porcine coronaviruses in feed and feed ingredients
title_fullStr Environmental persistence of porcine coronaviruses in feed and feed ingredients
title_full_unstemmed Environmental persistence of porcine coronaviruses in feed and feed ingredients
title_short Environmental persistence of porcine coronaviruses in feed and feed ingredients
title_sort environmental persistence of porcine coronaviruses in feed and feed ingredients
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5443540/
https://www.ncbi.nlm.nih.gov/pubmed/28542235
http://dx.doi.org/10.1371/journal.pone.0178094
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