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Characterization of porcine simple sequence repeat variation on a population scale with genome resequencing data
Simple sequence repeats (SSRs) are used as polymorphic molecular markers in many species. They contribute very important functional variations in a range of complex traits; however, little is known about the variation of most SSRs in pig populations. Here, using genome resequencing data, we identifi...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5443785/ https://www.ncbi.nlm.nih.gov/pubmed/28539617 http://dx.doi.org/10.1038/s41598-017-02600-8 |
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author | Liu, Congcong Liu, Yan Zhang, Xinyi Xu, Xuewen Zhao, Shuhong |
author_facet | Liu, Congcong Liu, Yan Zhang, Xinyi Xu, Xuewen Zhao, Shuhong |
author_sort | Liu, Congcong |
collection | PubMed |
description | Simple sequence repeats (SSRs) are used as polymorphic molecular markers in many species. They contribute very important functional variations in a range of complex traits; however, little is known about the variation of most SSRs in pig populations. Here, using genome resequencing data, we identified ~0.63 million polymorphic SSR loci from more than 100 individuals. Through intensive analysis of this dataset, we found that the SSR motif composition, motif length, total length of alleles and distribution of alleles all contribute to SSR variability. Furthermore, we found that CG-containing SSRs displayed significantly lower polymorphism and higher cross-species conservation. With a rigorous filter procedure, we provided a catalogue of 16,527 high-quality polymorphic SSRs, which displayed reliable results for the analysis of phylogenetic relationships and provided valuable summary statistics for 30 individuals equally selected from eight local Chinese pig breeds, six commercial lean pig breeds and Chinese wild boars. In addition, from the high-quality polymorphic SSR catalogue, we identified four loci with potential loss-of-function alleles. Overall, these analyses provide a valuable catalogue of polymorphic SSRs to the existing pig genetic variation database, and we believe this catalogue could be used for future genome-wide genetic analysis. |
format | Online Article Text |
id | pubmed-5443785 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-54437852017-05-26 Characterization of porcine simple sequence repeat variation on a population scale with genome resequencing data Liu, Congcong Liu, Yan Zhang, Xinyi Xu, Xuewen Zhao, Shuhong Sci Rep Article Simple sequence repeats (SSRs) are used as polymorphic molecular markers in many species. They contribute very important functional variations in a range of complex traits; however, little is known about the variation of most SSRs in pig populations. Here, using genome resequencing data, we identified ~0.63 million polymorphic SSR loci from more than 100 individuals. Through intensive analysis of this dataset, we found that the SSR motif composition, motif length, total length of alleles and distribution of alleles all contribute to SSR variability. Furthermore, we found that CG-containing SSRs displayed significantly lower polymorphism and higher cross-species conservation. With a rigorous filter procedure, we provided a catalogue of 16,527 high-quality polymorphic SSRs, which displayed reliable results for the analysis of phylogenetic relationships and provided valuable summary statistics for 30 individuals equally selected from eight local Chinese pig breeds, six commercial lean pig breeds and Chinese wild boars. In addition, from the high-quality polymorphic SSR catalogue, we identified four loci with potential loss-of-function alleles. Overall, these analyses provide a valuable catalogue of polymorphic SSRs to the existing pig genetic variation database, and we believe this catalogue could be used for future genome-wide genetic analysis. Nature Publishing Group UK 2017-05-24 /pmc/articles/PMC5443785/ /pubmed/28539617 http://dx.doi.org/10.1038/s41598-017-02600-8 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Liu, Congcong Liu, Yan Zhang, Xinyi Xu, Xuewen Zhao, Shuhong Characterization of porcine simple sequence repeat variation on a population scale with genome resequencing data |
title | Characterization of porcine simple sequence repeat variation on a population scale with genome resequencing data |
title_full | Characterization of porcine simple sequence repeat variation on a population scale with genome resequencing data |
title_fullStr | Characterization of porcine simple sequence repeat variation on a population scale with genome resequencing data |
title_full_unstemmed | Characterization of porcine simple sequence repeat variation on a population scale with genome resequencing data |
title_short | Characterization of porcine simple sequence repeat variation on a population scale with genome resequencing data |
title_sort | characterization of porcine simple sequence repeat variation on a population scale with genome resequencing data |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5443785/ https://www.ncbi.nlm.nih.gov/pubmed/28539617 http://dx.doi.org/10.1038/s41598-017-02600-8 |
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