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An episomal vector-based CRISPR/Cas9 system for highly efficient gene knockout in human pluripotent stem cells

Human pluripotent stem cells (hPSCs) represent a unique opportunity for understanding the molecular mechanisms underlying complex traits and diseases. CRISPR/Cas9 is a powerful tool to introduce genetic mutations into the hPSCs for loss-of-function studies. Here, we developed an episomal vector-base...

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Detalles Bibliográficos
Autores principales:	Xie, Yifang, Wang, Daqi, Lan, Feng, Wei, Gang, Ni, Ting, Chai, Renjie, Liu, Dong, Hu, Shijun, Li, Mingqing, Li, Dajin, Wang, Hongyan, Wang, Yongming
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2017
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5443789/ https://www.ncbi.nlm.nih.gov/pubmed/28539611 http://dx.doi.org/10.1038/s41598-017-02456-y

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