Low level of Lck kinase in Th2 cells limits expression of CD4 co-receptor and S73 phosphorylation of transcription factor c-Jun

The Src-family tyrosine kinase Lck is an enzyme associated with the CD4 and CD8 co-receptors and promoting signaling through the T cell receptor (TCR) complex. The levels of Lck expression and activity change during the development and differentiation of T cells. Here we show that Lck expression is...

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Autores principales: Shebzukhov, Yury V., Stanislawiak, Silke, Bezhaeva, Taisiya R., Nedospasov, Sergei A., Kuprash, Dmitry V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5443812/
https://www.ncbi.nlm.nih.gov/pubmed/28539628
http://dx.doi.org/10.1038/s41598-017-02553-y
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author Shebzukhov, Yury V.
Stanislawiak, Silke
Bezhaeva, Taisiya R.
Nedospasov, Sergei A.
Kuprash, Dmitry V.
author_facet Shebzukhov, Yury V.
Stanislawiak, Silke
Bezhaeva, Taisiya R.
Nedospasov, Sergei A.
Kuprash, Dmitry V.
author_sort Shebzukhov, Yury V.
collection PubMed
description The Src-family tyrosine kinase Lck is an enzyme associated with the CD4 and CD8 co-receptors and promoting signaling through the T cell receptor (TCR) complex. The levels of Lck expression and activity change during the development and differentiation of T cells. Here we show that Lck expression is higher in Th1 cells as compared to Th2 cells. Ectopic overexpression of Lck in Th2 cells results in increased expression of CD4 co-receptor and enhanced S73 phosphorylation of transcription factor c-Jun. Our findings indicate that TCR-mediated signaling in Th2 cells may be directly attenuated by Lck protein expression level.
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spelling pubmed-54438122017-05-26 Low level of Lck kinase in Th2 cells limits expression of CD4 co-receptor and S73 phosphorylation of transcription factor c-Jun Shebzukhov, Yury V. Stanislawiak, Silke Bezhaeva, Taisiya R. Nedospasov, Sergei A. Kuprash, Dmitry V. Sci Rep Article The Src-family tyrosine kinase Lck is an enzyme associated with the CD4 and CD8 co-receptors and promoting signaling through the T cell receptor (TCR) complex. The levels of Lck expression and activity change during the development and differentiation of T cells. Here we show that Lck expression is higher in Th1 cells as compared to Th2 cells. Ectopic overexpression of Lck in Th2 cells results in increased expression of CD4 co-receptor and enhanced S73 phosphorylation of transcription factor c-Jun. Our findings indicate that TCR-mediated signaling in Th2 cells may be directly attenuated by Lck protein expression level. Nature Publishing Group UK 2017-05-24 /pmc/articles/PMC5443812/ /pubmed/28539628 http://dx.doi.org/10.1038/s41598-017-02553-y Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Shebzukhov, Yury V.
Stanislawiak, Silke
Bezhaeva, Taisiya R.
Nedospasov, Sergei A.
Kuprash, Dmitry V.
Low level of Lck kinase in Th2 cells limits expression of CD4 co-receptor and S73 phosphorylation of transcription factor c-Jun
title Low level of Lck kinase in Th2 cells limits expression of CD4 co-receptor and S73 phosphorylation of transcription factor c-Jun
title_full Low level of Lck kinase in Th2 cells limits expression of CD4 co-receptor and S73 phosphorylation of transcription factor c-Jun
title_fullStr Low level of Lck kinase in Th2 cells limits expression of CD4 co-receptor and S73 phosphorylation of transcription factor c-Jun
title_full_unstemmed Low level of Lck kinase in Th2 cells limits expression of CD4 co-receptor and S73 phosphorylation of transcription factor c-Jun
title_short Low level of Lck kinase in Th2 cells limits expression of CD4 co-receptor and S73 phosphorylation of transcription factor c-Jun
title_sort low level of lck kinase in th2 cells limits expression of cd4 co-receptor and s73 phosphorylation of transcription factor c-jun
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5443812/
https://www.ncbi.nlm.nih.gov/pubmed/28539628
http://dx.doi.org/10.1038/s41598-017-02553-y
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