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Characterization of the Adult Head Transcriptome and Identification of Migration and Olfaction Genes in the Oriental Armyworm Mythimna separate
The oriental armyworm Mythimna separate is an economically important insect with a wide distribution and strong migratory activity. However, knowledge about the molecular mechanisms regulating the physiological and behavioural responses of the oriental armyworm is scarce. In the present study, we to...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5443819/ https://www.ncbi.nlm.nih.gov/pubmed/28539591 http://dx.doi.org/10.1038/s41598-017-02513-6 |
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author | Bian, Hai-Xu Ma, Hong-Fang Zheng, Xi-Xi Peng, Ming-Hui Li, Yu-Ping Su, Jun-Fang Wang, Huan Li, Qun Xia, Run-Xi Liu, Yan-Qun Jiang, Xing-Fu |
author_facet | Bian, Hai-Xu Ma, Hong-Fang Zheng, Xi-Xi Peng, Ming-Hui Li, Yu-Ping Su, Jun-Fang Wang, Huan Li, Qun Xia, Run-Xi Liu, Yan-Qun Jiang, Xing-Fu |
author_sort | Bian, Hai-Xu |
collection | PubMed |
description | The oriental armyworm Mythimna separate is an economically important insect with a wide distribution and strong migratory activity. However, knowledge about the molecular mechanisms regulating the physiological and behavioural responses of the oriental armyworm is scarce. In the present study, we took a transcriptomic approach to characterize the gene network in the adult head of M. separate. The sequencing and de novo assembly yielded 63,499 transcripts, which were further assembled into 46,459 unigenes with an N50 of 1,153 bp. In the head transcriptome data, unigenes involved in the ‘signal transduction mechanism’ are the most abundant. In total, 937 signal transduction unigenes were assigned to 22 signalling pathways. The circadian clock, melanin synthesis, and non-receptor protein of olfactory gene families were then identified, and phylogenetic analyses were performed with these M. separate genes, the model insect Bombyx mori and other insects. Furthermore, 1,372 simple sequence repeats of 2–6 bp in unit length were identified. The transcriptome data represent a comprehensive molecular resource for the adult head of M. separate, and these identified genes can be valid targets for further gene function research to address the molecular mechanisms regulating the migratory and olfaction genes of the oriental armyworm. |
format | Online Article Text |
id | pubmed-5443819 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-54438192017-05-26 Characterization of the Adult Head Transcriptome and Identification of Migration and Olfaction Genes in the Oriental Armyworm Mythimna separate Bian, Hai-Xu Ma, Hong-Fang Zheng, Xi-Xi Peng, Ming-Hui Li, Yu-Ping Su, Jun-Fang Wang, Huan Li, Qun Xia, Run-Xi Liu, Yan-Qun Jiang, Xing-Fu Sci Rep Article The oriental armyworm Mythimna separate is an economically important insect with a wide distribution and strong migratory activity. However, knowledge about the molecular mechanisms regulating the physiological and behavioural responses of the oriental armyworm is scarce. In the present study, we took a transcriptomic approach to characterize the gene network in the adult head of M. separate. The sequencing and de novo assembly yielded 63,499 transcripts, which were further assembled into 46,459 unigenes with an N50 of 1,153 bp. In the head transcriptome data, unigenes involved in the ‘signal transduction mechanism’ are the most abundant. In total, 937 signal transduction unigenes were assigned to 22 signalling pathways. The circadian clock, melanin synthesis, and non-receptor protein of olfactory gene families were then identified, and phylogenetic analyses were performed with these M. separate genes, the model insect Bombyx mori and other insects. Furthermore, 1,372 simple sequence repeats of 2–6 bp in unit length were identified. The transcriptome data represent a comprehensive molecular resource for the adult head of M. separate, and these identified genes can be valid targets for further gene function research to address the molecular mechanisms regulating the migratory and olfaction genes of the oriental armyworm. Nature Publishing Group UK 2017-05-24 /pmc/articles/PMC5443819/ /pubmed/28539591 http://dx.doi.org/10.1038/s41598-017-02513-6 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Bian, Hai-Xu Ma, Hong-Fang Zheng, Xi-Xi Peng, Ming-Hui Li, Yu-Ping Su, Jun-Fang Wang, Huan Li, Qun Xia, Run-Xi Liu, Yan-Qun Jiang, Xing-Fu Characterization of the Adult Head Transcriptome and Identification of Migration and Olfaction Genes in the Oriental Armyworm Mythimna separate |
title | Characterization of the Adult Head Transcriptome and Identification of Migration and Olfaction Genes in the Oriental Armyworm Mythimna separate |
title_full | Characterization of the Adult Head Transcriptome and Identification of Migration and Olfaction Genes in the Oriental Armyworm Mythimna separate |
title_fullStr | Characterization of the Adult Head Transcriptome and Identification of Migration and Olfaction Genes in the Oriental Armyworm Mythimna separate |
title_full_unstemmed | Characterization of the Adult Head Transcriptome and Identification of Migration and Olfaction Genes in the Oriental Armyworm Mythimna separate |
title_short | Characterization of the Adult Head Transcriptome and Identification of Migration and Olfaction Genes in the Oriental Armyworm Mythimna separate |
title_sort | characterization of the adult head transcriptome and identification of migration and olfaction genes in the oriental armyworm mythimna separate |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5443819/ https://www.ncbi.nlm.nih.gov/pubmed/28539591 http://dx.doi.org/10.1038/s41598-017-02513-6 |
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