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Regulation of chloroplast and nucleomorph replication by the cell cycle in the cryptophyte Guillardia theta
The chloroplasts of cryptophytes arose through a secondary endosymbiotic event in which a red algal endosymbiont was integrated into a previously nonphotosynthetic eukaryote. The cryptophytes retain a remnant of the endosymbiont nucleus (nucleomorph) that is replicated once in the cell cycle along w...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5443833/ https://www.ncbi.nlm.nih.gov/pubmed/28539635 http://dx.doi.org/10.1038/s41598-017-02668-2 |
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author | Onuma, Ryo Mishra, Neha Miyagishima, Shin-ya |
author_facet | Onuma, Ryo Mishra, Neha Miyagishima, Shin-ya |
author_sort | Onuma, Ryo |
collection | PubMed |
description | The chloroplasts of cryptophytes arose through a secondary endosymbiotic event in which a red algal endosymbiont was integrated into a previously nonphotosynthetic eukaryote. The cryptophytes retain a remnant of the endosymbiont nucleus (nucleomorph) that is replicated once in the cell cycle along with the chloroplast. To understand how the chloroplast, nucleomorph and host cell divide in a coordinated manner, we examined the expression of genes/proteins that are related to nucleomorph replication and chloroplast division as well as the timing of nuclear and nucleomorph DNA synthesis in the cryptophyte Guillardia theta. Nucleus-encoded nucleomorph HISTONE H2A mRNA specifically accumulated during the nuclear S phase. In contrast, nucleomorph-encoded genes/proteins that are related to nucleomorph replication and chloroplast division (FtsZ) are constantly expressed throughout the cell cycle. The results of this study and previous studies on chlorarachniophytes suggest that there was a common evolutionary pattern in which an endosymbiont lost its replication cycle-dependent transcription while cell-cycle-dependent transcriptional regulation of host nuclear genes came to restrict the timing of nucleomorph replication and chloroplast division. |
format | Online Article Text |
id | pubmed-5443833 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-54438332017-05-26 Regulation of chloroplast and nucleomorph replication by the cell cycle in the cryptophyte Guillardia theta Onuma, Ryo Mishra, Neha Miyagishima, Shin-ya Sci Rep Article The chloroplasts of cryptophytes arose through a secondary endosymbiotic event in which a red algal endosymbiont was integrated into a previously nonphotosynthetic eukaryote. The cryptophytes retain a remnant of the endosymbiont nucleus (nucleomorph) that is replicated once in the cell cycle along with the chloroplast. To understand how the chloroplast, nucleomorph and host cell divide in a coordinated manner, we examined the expression of genes/proteins that are related to nucleomorph replication and chloroplast division as well as the timing of nuclear and nucleomorph DNA synthesis in the cryptophyte Guillardia theta. Nucleus-encoded nucleomorph HISTONE H2A mRNA specifically accumulated during the nuclear S phase. In contrast, nucleomorph-encoded genes/proteins that are related to nucleomorph replication and chloroplast division (FtsZ) are constantly expressed throughout the cell cycle. The results of this study and previous studies on chlorarachniophytes suggest that there was a common evolutionary pattern in which an endosymbiont lost its replication cycle-dependent transcription while cell-cycle-dependent transcriptional regulation of host nuclear genes came to restrict the timing of nucleomorph replication and chloroplast division. Nature Publishing Group UK 2017-05-24 /pmc/articles/PMC5443833/ /pubmed/28539635 http://dx.doi.org/10.1038/s41598-017-02668-2 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Onuma, Ryo Mishra, Neha Miyagishima, Shin-ya Regulation of chloroplast and nucleomorph replication by the cell cycle in the cryptophyte Guillardia theta |
title | Regulation of chloroplast and nucleomorph replication by the cell cycle in the cryptophyte Guillardia theta |
title_full | Regulation of chloroplast and nucleomorph replication by the cell cycle in the cryptophyte Guillardia theta |
title_fullStr | Regulation of chloroplast and nucleomorph replication by the cell cycle in the cryptophyte Guillardia theta |
title_full_unstemmed | Regulation of chloroplast and nucleomorph replication by the cell cycle in the cryptophyte Guillardia theta |
title_short | Regulation of chloroplast and nucleomorph replication by the cell cycle in the cryptophyte Guillardia theta |
title_sort | regulation of chloroplast and nucleomorph replication by the cell cycle in the cryptophyte guillardia theta |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5443833/ https://www.ncbi.nlm.nih.gov/pubmed/28539635 http://dx.doi.org/10.1038/s41598-017-02668-2 |
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