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Repeat-length variation in a wheat cellulose synthase-like gene is associated with altered tiller number and stem cell wall composition

The tiller inhibition gene (tin) that reduces tillering in wheat (Triticum aestivum) is also associated with large spikes, increased grain weight, and thick leaves and stems. In this study, comparison of near-isogenic lines (NILs) revealed changes in stem morphology, cell wall composition, and stem...

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Autores principales: Hyles, J., Vautrin, S., Pettolino, F., MacMillan, C., Stachurski, Z., Breen, J., Berges, H., Wicker, T., Spielmeyer, W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5444437/
https://www.ncbi.nlm.nih.gov/pubmed/28369427
http://dx.doi.org/10.1093/jxb/erx051
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author Hyles, J.
Vautrin, S.
Pettolino, F.
MacMillan, C.
Stachurski, Z.
Breen, J.
Berges, H.
Wicker, T.
Spielmeyer, W.
author_facet Hyles, J.
Vautrin, S.
Pettolino, F.
MacMillan, C.
Stachurski, Z.
Breen, J.
Berges, H.
Wicker, T.
Spielmeyer, W.
author_sort Hyles, J.
collection PubMed
description The tiller inhibition gene (tin) that reduces tillering in wheat (Triticum aestivum) is also associated with large spikes, increased grain weight, and thick leaves and stems. In this study, comparison of near-isogenic lines (NILs) revealed changes in stem morphology, cell wall composition, and stem strength. Microscopic analysis of stem cross-sections and chemical analysis of stem tissue indicated that cell walls in tin lines were thicker and more lignified than in free-tillering NILs. Increased lignification was associated with stronger stems in tin plants. A candidate gene for tin was identified through map-based cloning and was predicted to encode a cellulose synthase-like (Csl) protein with homology to members of the CslA clade. Dinucleotide repeat-length polymorphism in the 5′UTR region of the Csl gene was associated with tiller number in diverse wheat germplasm and linked to expression differences of Csl transcripts between NILs. We propose that regulation of Csl transcript and/or protein levels affects carbon partitioning throughout the plant, which plays a key role in the tin phenotype.
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spelling pubmed-54444372017-05-31 Repeat-length variation in a wheat cellulose synthase-like gene is associated with altered tiller number and stem cell wall composition Hyles, J. Vautrin, S. Pettolino, F. MacMillan, C. Stachurski, Z. Breen, J. Berges, H. Wicker, T. Spielmeyer, W. J Exp Bot Research Paper The tiller inhibition gene (tin) that reduces tillering in wheat (Triticum aestivum) is also associated with large spikes, increased grain weight, and thick leaves and stems. In this study, comparison of near-isogenic lines (NILs) revealed changes in stem morphology, cell wall composition, and stem strength. Microscopic analysis of stem cross-sections and chemical analysis of stem tissue indicated that cell walls in tin lines were thicker and more lignified than in free-tillering NILs. Increased lignification was associated with stronger stems in tin plants. A candidate gene for tin was identified through map-based cloning and was predicted to encode a cellulose synthase-like (Csl) protein with homology to members of the CslA clade. Dinucleotide repeat-length polymorphism in the 5′UTR region of the Csl gene was associated with tiller number in diverse wheat germplasm and linked to expression differences of Csl transcripts between NILs. We propose that regulation of Csl transcript and/or protein levels affects carbon partitioning throughout the plant, which plays a key role in the tin phenotype. Oxford University Press 2017-03-01 2017-03-28 /pmc/articles/PMC5444437/ /pubmed/28369427 http://dx.doi.org/10.1093/jxb/erx051 Text en © The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Hyles, J.
Vautrin, S.
Pettolino, F.
MacMillan, C.
Stachurski, Z.
Breen, J.
Berges, H.
Wicker, T.
Spielmeyer, W.
Repeat-length variation in a wheat cellulose synthase-like gene is associated with altered tiller number and stem cell wall composition
title Repeat-length variation in a wheat cellulose synthase-like gene is associated with altered tiller number and stem cell wall composition
title_full Repeat-length variation in a wheat cellulose synthase-like gene is associated with altered tiller number and stem cell wall composition
title_fullStr Repeat-length variation in a wheat cellulose synthase-like gene is associated with altered tiller number and stem cell wall composition
title_full_unstemmed Repeat-length variation in a wheat cellulose synthase-like gene is associated with altered tiller number and stem cell wall composition
title_short Repeat-length variation in a wheat cellulose synthase-like gene is associated with altered tiller number and stem cell wall composition
title_sort repeat-length variation in a wheat cellulose synthase-like gene is associated with altered tiller number and stem cell wall composition
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5444437/
https://www.ncbi.nlm.nih.gov/pubmed/28369427
http://dx.doi.org/10.1093/jxb/erx051
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