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Flagellin peptide flg22 gains access to long-distance trafficking in Arabidopsis via its receptor, FLS2
Diverse pathogen-derived molecules, such as bacterial flagellin and its conserved peptide flg22, are recognized in plants via plasma membrane receptors and induce both local and systemic immune responses. The fate of such ligands was unknown: whether and by what mechanism(s) they enter plant cells a...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5444442/ https://www.ncbi.nlm.nih.gov/pubmed/28521013 http://dx.doi.org/10.1093/jxb/erx060 |
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author | Jelenska, Joanna Davern, Sandra M. Standaert, Robert F. Mirzadeh, Saed Greenberg, Jean T. |
author_facet | Jelenska, Joanna Davern, Sandra M. Standaert, Robert F. Mirzadeh, Saed Greenberg, Jean T. |
author_sort | Jelenska, Joanna |
collection | PubMed |
description | Diverse pathogen-derived molecules, such as bacterial flagellin and its conserved peptide flg22, are recognized in plants via plasma membrane receptors and induce both local and systemic immune responses. The fate of such ligands was unknown: whether and by what mechanism(s) they enter plant cells and whether they are transported to distal tissues. We used biologically active fluorophore and radiolabeled peptides to establish that flg22 moves to distal organs with the closest vascular connections. Remarkably, entry into the plant cell via endocytosis together with the FLS2 receptor is needed for delivery to vascular tissue and long-distance transport of flg22. This contrasts with known routes of long distance transport of other non-cell-permeant molecules in plants, which require membrane-localized transporters for entry to vascular tissue. Thus, a plasma membrane receptor acts as a transporter to enable access of its ligand to distal trafficking routes. |
format | Online Article Text |
id | pubmed-5444442 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-54444422017-05-31 Flagellin peptide flg22 gains access to long-distance trafficking in Arabidopsis via its receptor, FLS2 Jelenska, Joanna Davern, Sandra M. Standaert, Robert F. Mirzadeh, Saed Greenberg, Jean T. J Exp Bot Research Paper Diverse pathogen-derived molecules, such as bacterial flagellin and its conserved peptide flg22, are recognized in plants via plasma membrane receptors and induce both local and systemic immune responses. The fate of such ligands was unknown: whether and by what mechanism(s) they enter plant cells and whether they are transported to distal tissues. We used biologically active fluorophore and radiolabeled peptides to establish that flg22 moves to distal organs with the closest vascular connections. Remarkably, entry into the plant cell via endocytosis together with the FLS2 receptor is needed for delivery to vascular tissue and long-distance transport of flg22. This contrasts with known routes of long distance transport of other non-cell-permeant molecules in plants, which require membrane-localized transporters for entry to vascular tissue. Thus, a plasma membrane receptor acts as a transporter to enable access of its ligand to distal trafficking routes. Oxford University Press 2017-03-01 2017-04-28 /pmc/articles/PMC5444442/ /pubmed/28521013 http://dx.doi.org/10.1093/jxb/erx060 Text en © The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Paper Jelenska, Joanna Davern, Sandra M. Standaert, Robert F. Mirzadeh, Saed Greenberg, Jean T. Flagellin peptide flg22 gains access to long-distance trafficking in Arabidopsis via its receptor, FLS2 |
title | Flagellin peptide flg22 gains access to long-distance trafficking in Arabidopsis via its receptor, FLS2 |
title_full | Flagellin peptide flg22 gains access to long-distance trafficking in Arabidopsis via its receptor, FLS2 |
title_fullStr | Flagellin peptide flg22 gains access to long-distance trafficking in Arabidopsis via its receptor, FLS2 |
title_full_unstemmed | Flagellin peptide flg22 gains access to long-distance trafficking in Arabidopsis via its receptor, FLS2 |
title_short | Flagellin peptide flg22 gains access to long-distance trafficking in Arabidopsis via its receptor, FLS2 |
title_sort | flagellin peptide flg22 gains access to long-distance trafficking in arabidopsis via its receptor, fls2 |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5444442/ https://www.ncbi.nlm.nih.gov/pubmed/28521013 http://dx.doi.org/10.1093/jxb/erx060 |
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