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Rapid deployment of a mobile biosafety level-3 laboratory in Sierra Leone during the 2014 Ebola virus epidemic
BACKGROUND: Ebola virus emerged in West Africa in December 2013. The high population mobility and poor public health infrastructure in this region led to the development of the largest Ebola virus disease (EVD) outbreak to date. METHODOLOGY/PRINCIPAL FINDINGS: On September 26, 2014, China dispatched...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5444861/ https://www.ncbi.nlm.nih.gov/pubmed/28505171 http://dx.doi.org/10.1371/journal.pntd.0005622 |
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author | Zhang, Yi Gong, Yan Wang, Chengyu Liu, Wensen Wang, Zhongyi Xia, Zhiping Bu, Zhaoyang Lu, Huijun Sun, Yang Zhang, Xiaoguang Cao, Yuxi Yang, Fan Su, Haoxiang Hu, Yi Deng, Yongqiang Zhou, Bo Zhao, Zongzheng Fu, Yingying Kargbo, David Dafae, Foday Kargbo, Brima Kanu, Alex Liu, Linna Qian, Jun Guo, Zhendong |
author_facet | Zhang, Yi Gong, Yan Wang, Chengyu Liu, Wensen Wang, Zhongyi Xia, Zhiping Bu, Zhaoyang Lu, Huijun Sun, Yang Zhang, Xiaoguang Cao, Yuxi Yang, Fan Su, Haoxiang Hu, Yi Deng, Yongqiang Zhou, Bo Zhao, Zongzheng Fu, Yingying Kargbo, David Dafae, Foday Kargbo, Brima Kanu, Alex Liu, Linna Qian, Jun Guo, Zhendong |
author_sort | Zhang, Yi |
collection | PubMed |
description | BACKGROUND: Ebola virus emerged in West Africa in December 2013. The high population mobility and poor public health infrastructure in this region led to the development of the largest Ebola virus disease (EVD) outbreak to date. METHODOLOGY/PRINCIPAL FINDINGS: On September 26, 2014, China dispatched a Mobile Biosafety Level-3 Laboratory (MBSL-3 Lab) and a well-trained diagnostic team to Sierra Leone to assist in EVD diagnosis using quantitative real-time PCR, which allowed the diagnosis of suspected EVD cases in less than 4 hours from the time of sample receiving. This laboratory was composed of three container vehicles equipped with advanced ventilation system, communication system, electricity and gas supply system. We strictly applied multiple safety precautions to reduce exposure risks. Personnel, materials, water and air flow management were the key elements of the biosafety measures in the MBSL-3 Lab. Air samples were regularly collected from the MBSL-3 Lab, but no evidence of Ebola virus infectious aerosols was detected. Potentially contaminated objects were also tested by collecting swabs. On one occasion, a pipette tested positive for EVD. A total of 1,635 suspected EVD cases (824 positive [50.4%]) were tested from September 28 to November 11, 2014, and no member of the diagnostic team was infected with Ebola virus or other pathogens, including Lassa fever. The specimens tested included blood (69.2%) and oral swabs (30.8%) with positivity rates of 54.2% and 41.9%, respectively. The China mobile laboratory was thus instrumental in the EVD outbreak response by providing timely and reliable diagnostics. CONCLUSIONS/SIGNIFICANCE: The MBSL-3 Lab significantly contributed to establishing a suitable laboratory response capacity during the emergence of EVD in Sierra Leone. |
format | Online Article Text |
id | pubmed-5444861 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-54448612017-06-06 Rapid deployment of a mobile biosafety level-3 laboratory in Sierra Leone during the 2014 Ebola virus epidemic Zhang, Yi Gong, Yan Wang, Chengyu Liu, Wensen Wang, Zhongyi Xia, Zhiping Bu, Zhaoyang Lu, Huijun Sun, Yang Zhang, Xiaoguang Cao, Yuxi Yang, Fan Su, Haoxiang Hu, Yi Deng, Yongqiang Zhou, Bo Zhao, Zongzheng Fu, Yingying Kargbo, David Dafae, Foday Kargbo, Brima Kanu, Alex Liu, Linna Qian, Jun Guo, Zhendong PLoS Negl Trop Dis Research Article BACKGROUND: Ebola virus emerged in West Africa in December 2013. The high population mobility and poor public health infrastructure in this region led to the development of the largest Ebola virus disease (EVD) outbreak to date. METHODOLOGY/PRINCIPAL FINDINGS: On September 26, 2014, China dispatched a Mobile Biosafety Level-3 Laboratory (MBSL-3 Lab) and a well-trained diagnostic team to Sierra Leone to assist in EVD diagnosis using quantitative real-time PCR, which allowed the diagnosis of suspected EVD cases in less than 4 hours from the time of sample receiving. This laboratory was composed of three container vehicles equipped with advanced ventilation system, communication system, electricity and gas supply system. We strictly applied multiple safety precautions to reduce exposure risks. Personnel, materials, water and air flow management were the key elements of the biosafety measures in the MBSL-3 Lab. Air samples were regularly collected from the MBSL-3 Lab, but no evidence of Ebola virus infectious aerosols was detected. Potentially contaminated objects were also tested by collecting swabs. On one occasion, a pipette tested positive for EVD. A total of 1,635 suspected EVD cases (824 positive [50.4%]) were tested from September 28 to November 11, 2014, and no member of the diagnostic team was infected with Ebola virus or other pathogens, including Lassa fever. The specimens tested included blood (69.2%) and oral swabs (30.8%) with positivity rates of 54.2% and 41.9%, respectively. The China mobile laboratory was thus instrumental in the EVD outbreak response by providing timely and reliable diagnostics. CONCLUSIONS/SIGNIFICANCE: The MBSL-3 Lab significantly contributed to establishing a suitable laboratory response capacity during the emergence of EVD in Sierra Leone. Public Library of Science 2017-05-15 /pmc/articles/PMC5444861/ /pubmed/28505171 http://dx.doi.org/10.1371/journal.pntd.0005622 Text en © 2017 Zhang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Zhang, Yi Gong, Yan Wang, Chengyu Liu, Wensen Wang, Zhongyi Xia, Zhiping Bu, Zhaoyang Lu, Huijun Sun, Yang Zhang, Xiaoguang Cao, Yuxi Yang, Fan Su, Haoxiang Hu, Yi Deng, Yongqiang Zhou, Bo Zhao, Zongzheng Fu, Yingying Kargbo, David Dafae, Foday Kargbo, Brima Kanu, Alex Liu, Linna Qian, Jun Guo, Zhendong Rapid deployment of a mobile biosafety level-3 laboratory in Sierra Leone during the 2014 Ebola virus epidemic |
title | Rapid deployment of a mobile biosafety level-3 laboratory in Sierra Leone during the 2014 Ebola virus epidemic |
title_full | Rapid deployment of a mobile biosafety level-3 laboratory in Sierra Leone during the 2014 Ebola virus epidemic |
title_fullStr | Rapid deployment of a mobile biosafety level-3 laboratory in Sierra Leone during the 2014 Ebola virus epidemic |
title_full_unstemmed | Rapid deployment of a mobile biosafety level-3 laboratory in Sierra Leone during the 2014 Ebola virus epidemic |
title_short | Rapid deployment of a mobile biosafety level-3 laboratory in Sierra Leone during the 2014 Ebola virus epidemic |
title_sort | rapid deployment of a mobile biosafety level-3 laboratory in sierra leone during the 2014 ebola virus epidemic |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5444861/ https://www.ncbi.nlm.nih.gov/pubmed/28505171 http://dx.doi.org/10.1371/journal.pntd.0005622 |
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