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Neuronal Voltage Gated Potassium Channels May Modulate Nitric Oxide Synthesis in Corpus Cavernosum
Potassium channels (K(+)Ch) in corpus cavernosum play an important role in the regulation of erection. Nitric oxide (NO) acts through opening of K(+)Ch leading to hyperpolarization and relaxation. Aim : This study aims to update knowledge about the role of voltage-gated K(+)Ch (K(V)) channels in ere...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5445172/ https://www.ncbi.nlm.nih.gov/pubmed/28603495 http://dx.doi.org/10.3389/fphar.2017.00297 |
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author | Senbel, Amira M. Abd Elmoneim, Heba M. Sharabi, Fouad M. Mohy El-Din, Mahmoud M. |
author_facet | Senbel, Amira M. Abd Elmoneim, Heba M. Sharabi, Fouad M. Mohy El-Din, Mahmoud M. |
author_sort | Senbel, Amira M. |
collection | PubMed |
description | Potassium channels (K(+)Ch) in corpus cavernosum play an important role in the regulation of erection. Nitric oxide (NO) acts through opening of K(+)Ch leading to hyperpolarization and relaxation. Aim : This study aims to update knowledge about the role of voltage-gated K(+)Ch (K(V)) channels in erectile machinery and investigate their role in the control of NO action &/or synthesis in the corpus cavernosum. Methods : Tension studies using isolated rabbit corpus cavernosum (CC) strips and rat anococcygeus muscle were conducted. Results are expressed as mean ± SEM. Results : Electric field stimulation (EFS, 2–16 Hz) evoked frequency-dependent relaxations of the PE (phenylephrine)-precontracted CC strips. At 2 Hz, EFS-induced relaxation amounted to 73.17 ± 2.55% in presence 4-AP (10(−3) M) compared to 41.98 ± 1.45% as control. None of the other selective K(+)Ch blockers tested inhibited EFS-induced relaxation. 4-AP (10(−3)M) significantly attenuated ACh-induced relaxation of rabbit CC where dose-response curve was clearly shifted upward, and attenuated SNP- induced relaxation, for example, to 49.28 ± 4.52% compared to 65.53 ± 3.01% as control at 10(−6) M SNP. The potentiatory effect of 4-AP on EFS was abolished or reversed in presence of N(G)-nitro-L-arginine (L-NNA, non-selective nitric oxide synthase inhibitor, 10(−5)M, and 2 × 10(−4)M). Same results were observed in rat anococcygeus muscle which is a part of the erectile machinery in rats. Conclusion : This study provides evidence for the presence of prejunctional voltage-gated K(+)Ch in CC, the blockade of which may increase the neuronal synthesis of NO. |
format | Online Article Text |
id | pubmed-5445172 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-54451722017-06-09 Neuronal Voltage Gated Potassium Channels May Modulate Nitric Oxide Synthesis in Corpus Cavernosum Senbel, Amira M. Abd Elmoneim, Heba M. Sharabi, Fouad M. Mohy El-Din, Mahmoud M. Front Pharmacol Pharmacology Potassium channels (K(+)Ch) in corpus cavernosum play an important role in the regulation of erection. Nitric oxide (NO) acts through opening of K(+)Ch leading to hyperpolarization and relaxation. Aim : This study aims to update knowledge about the role of voltage-gated K(+)Ch (K(V)) channels in erectile machinery and investigate their role in the control of NO action &/or synthesis in the corpus cavernosum. Methods : Tension studies using isolated rabbit corpus cavernosum (CC) strips and rat anococcygeus muscle were conducted. Results are expressed as mean ± SEM. Results : Electric field stimulation (EFS, 2–16 Hz) evoked frequency-dependent relaxations of the PE (phenylephrine)-precontracted CC strips. At 2 Hz, EFS-induced relaxation amounted to 73.17 ± 2.55% in presence 4-AP (10(−3) M) compared to 41.98 ± 1.45% as control. None of the other selective K(+)Ch blockers tested inhibited EFS-induced relaxation. 4-AP (10(−3)M) significantly attenuated ACh-induced relaxation of rabbit CC where dose-response curve was clearly shifted upward, and attenuated SNP- induced relaxation, for example, to 49.28 ± 4.52% compared to 65.53 ± 3.01% as control at 10(−6) M SNP. The potentiatory effect of 4-AP on EFS was abolished or reversed in presence of N(G)-nitro-L-arginine (L-NNA, non-selective nitric oxide synthase inhibitor, 10(−5)M, and 2 × 10(−4)M). Same results were observed in rat anococcygeus muscle which is a part of the erectile machinery in rats. Conclusion : This study provides evidence for the presence of prejunctional voltage-gated K(+)Ch in CC, the blockade of which may increase the neuronal synthesis of NO. Frontiers Media S.A. 2017-05-26 /pmc/articles/PMC5445172/ /pubmed/28603495 http://dx.doi.org/10.3389/fphar.2017.00297 Text en Copyright © 2017 Senbel, Abd Elmoneim, Sharabi and Mohy El-Din. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Pharmacology Senbel, Amira M. Abd Elmoneim, Heba M. Sharabi, Fouad M. Mohy El-Din, Mahmoud M. Neuronal Voltage Gated Potassium Channels May Modulate Nitric Oxide Synthesis in Corpus Cavernosum |
title | Neuronal Voltage Gated Potassium Channels May Modulate Nitric Oxide Synthesis in Corpus Cavernosum |
title_full | Neuronal Voltage Gated Potassium Channels May Modulate Nitric Oxide Synthesis in Corpus Cavernosum |
title_fullStr | Neuronal Voltage Gated Potassium Channels May Modulate Nitric Oxide Synthesis in Corpus Cavernosum |
title_full_unstemmed | Neuronal Voltage Gated Potassium Channels May Modulate Nitric Oxide Synthesis in Corpus Cavernosum |
title_short | Neuronal Voltage Gated Potassium Channels May Modulate Nitric Oxide Synthesis in Corpus Cavernosum |
title_sort | neuronal voltage gated potassium channels may modulate nitric oxide synthesis in corpus cavernosum |
topic | Pharmacology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5445172/ https://www.ncbi.nlm.nih.gov/pubmed/28603495 http://dx.doi.org/10.3389/fphar.2017.00297 |
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