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A Fluorescence in Situ Hybridization Method To Quantify mRNA Translation by Visualizing Ribosome–mRNA Interactions in Single Cells

[Image: see text] Single-molecule fluorescence in situ hybridization (smFISH) is a simple and widely used method to measure mRNA transcript abundance and localization in single cells. A comparable single-molecule in situ method to measure mRNA translation would enable a more complete understanding o...

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Detalles Bibliográficos
Autores principales: Burke, Kelly S., Antilla, Katie A., Tirrell, David A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2017
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5445550/
https://www.ncbi.nlm.nih.gov/pubmed/28573204
http://dx.doi.org/10.1021/acscentsci.7b00048