A Novel Approach for Automated Analysis of Cell Attachment and Spreading Based on Backscattered Electron Imaging by Scanning Electron Microscopy

The development of new materials for biological application requires in vitro testing of cell/surface interactions. Cell adhesion and spreading are difficult to quantify as most materials are non-transparent and transmission microscopy cannot be used. Contrast in reflection microscopy is rather poor...

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Autores principales: Katsen-Globa, Alisa, Peter, Leonora, Zöllner, Susan, Dörge, Thomas, Daffertshofer, Martin, Preckel, Hartwig, Schmitt, Daniel, Zimmermann, Heiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International 2009
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5445736/
http://dx.doi.org/10.3390/ma2031402
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author Katsen-Globa, Alisa
Peter, Leonora
Zöllner, Susan
Dörge, Thomas
Daffertshofer, Martin
Preckel, Hartwig
Schmitt, Daniel
Zimmermann, Heiko
author_facet Katsen-Globa, Alisa
Peter, Leonora
Zöllner, Susan
Dörge, Thomas
Daffertshofer, Martin
Preckel, Hartwig
Schmitt, Daniel
Zimmermann, Heiko
author_sort Katsen-Globa, Alisa
collection PubMed
description The development of new materials for biological application requires in vitro testing of cell/surface interactions. Cell adhesion and spreading are difficult to quantify as most materials are non-transparent and transmission microscopy cannot be used. Contrast in reflection microscopy is rather poor. We propose an alternative method for the automated screening of cell attachment and spreading using backscattered electron imaging of scanning electron microscopy. The enhanced cell contrast permits study of cell/material interactions by little differences between cells and material.
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spelling pubmed-54457362017-07-28 A Novel Approach for Automated Analysis of Cell Attachment and Spreading Based on Backscattered Electron Imaging by Scanning Electron Microscopy Katsen-Globa, Alisa Peter, Leonora Zöllner, Susan Dörge, Thomas Daffertshofer, Martin Preckel, Hartwig Schmitt, Daniel Zimmermann, Heiko Materials (Basel) Article The development of new materials for biological application requires in vitro testing of cell/surface interactions. Cell adhesion and spreading are difficult to quantify as most materials are non-transparent and transmission microscopy cannot be used. Contrast in reflection microscopy is rather poor. We propose an alternative method for the automated screening of cell attachment and spreading using backscattered electron imaging of scanning electron microscopy. The enhanced cell contrast permits study of cell/material interactions by little differences between cells and material. Molecular Diversity Preservation International 2009-09-24 /pmc/articles/PMC5445736/ http://dx.doi.org/10.3390/ma2031402 Text en © 2009 by the authors. Licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Katsen-Globa, Alisa
Peter, Leonora
Zöllner, Susan
Dörge, Thomas
Daffertshofer, Martin
Preckel, Hartwig
Schmitt, Daniel
Zimmermann, Heiko
A Novel Approach for Automated Analysis of Cell Attachment and Spreading Based on Backscattered Electron Imaging by Scanning Electron Microscopy
title A Novel Approach for Automated Analysis of Cell Attachment and Spreading Based on Backscattered Electron Imaging by Scanning Electron Microscopy
title_full A Novel Approach for Automated Analysis of Cell Attachment and Spreading Based on Backscattered Electron Imaging by Scanning Electron Microscopy
title_fullStr A Novel Approach for Automated Analysis of Cell Attachment and Spreading Based on Backscattered Electron Imaging by Scanning Electron Microscopy
title_full_unstemmed A Novel Approach for Automated Analysis of Cell Attachment and Spreading Based on Backscattered Electron Imaging by Scanning Electron Microscopy
title_short A Novel Approach for Automated Analysis of Cell Attachment and Spreading Based on Backscattered Electron Imaging by Scanning Electron Microscopy
title_sort novel approach for automated analysis of cell attachment and spreading based on backscattered electron imaging by scanning electron microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5445736/
http://dx.doi.org/10.3390/ma2031402
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