Neural Stem Cell Spreading on Lipid Based Artificial Cell Surfaces, Characterized by Combined X-ray and Neutron Reflectometry

We developed a bioadhesive coating based on a synthetic peptide-conjugate (AK-cyclo[RGDfC]) which contains multiples of the arginyl-glycyl-aspartic acid (RGD) amino acid sequence. Biotinylated AK-cyclo[RGDfC] is bound to a supported lipid bilayer via a streptavidin interlayer. Layering, hydration an...

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Detalles Bibliográficos
Autores principales: Huth, Martin, Hertrich, Samira, Mezo, Gabor, Madarasz, Emilia, Nickel, Bert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5445775/
https://www.ncbi.nlm.nih.gov/pubmed/28883366
http://dx.doi.org/10.3390/ma3114994
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author Huth, Martin
Hertrich, Samira
Mezo, Gabor
Madarasz, Emilia
Nickel, Bert
author_facet Huth, Martin
Hertrich, Samira
Mezo, Gabor
Madarasz, Emilia
Nickel, Bert
author_sort Huth, Martin
collection PubMed
description We developed a bioadhesive coating based on a synthetic peptide-conjugate (AK-cyclo[RGDfC]) which contains multiples of the arginyl-glycyl-aspartic acid (RGD) amino acid sequence. Biotinylated AK-cyclo[RGDfC] is bound to a supported lipid bilayer via a streptavidin interlayer. Layering, hydration and packing of the coating is quantified by X-ray and neutron reflectometry experiments. AK-cyclo[RGDfC] binds to the streptavidin interlayer in a stretched-out on edge configuration. The highly packed configuration with only 12% water content maximizes the number of accessible adhesion sites. Enhanced cell spreading of neural stem cells was observed for AK-cyclo[RGDfC] functionalized bilayers. Due to the large variety of surfaces which can be coated by physisorption of lipid bilayers, this approach is of general interest for the fabrication of biocompatible surfaces.
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spelling pubmed-54457752017-07-28 Neural Stem Cell Spreading on Lipid Based Artificial Cell Surfaces, Characterized by Combined X-ray and Neutron Reflectometry Huth, Martin Hertrich, Samira Mezo, Gabor Madarasz, Emilia Nickel, Bert Materials (Basel) Article We developed a bioadhesive coating based on a synthetic peptide-conjugate (AK-cyclo[RGDfC]) which contains multiples of the arginyl-glycyl-aspartic acid (RGD) amino acid sequence. Biotinylated AK-cyclo[RGDfC] is bound to a supported lipid bilayer via a streptavidin interlayer. Layering, hydration and packing of the coating is quantified by X-ray and neutron reflectometry experiments. AK-cyclo[RGDfC] binds to the streptavidin interlayer in a stretched-out on edge configuration. The highly packed configuration with only 12% water content maximizes the number of accessible adhesion sites. Enhanced cell spreading of neural stem cells was observed for AK-cyclo[RGDfC] functionalized bilayers. Due to the large variety of surfaces which can be coated by physisorption of lipid bilayers, this approach is of general interest for the fabrication of biocompatible surfaces. MDPI 2010-11-22 /pmc/articles/PMC5445775/ /pubmed/28883366 http://dx.doi.org/10.3390/ma3114994 Text en © 2010 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Huth, Martin
Hertrich, Samira
Mezo, Gabor
Madarasz, Emilia
Nickel, Bert
Neural Stem Cell Spreading on Lipid Based Artificial Cell Surfaces, Characterized by Combined X-ray and Neutron Reflectometry
title Neural Stem Cell Spreading on Lipid Based Artificial Cell Surfaces, Characterized by Combined X-ray and Neutron Reflectometry
title_full Neural Stem Cell Spreading on Lipid Based Artificial Cell Surfaces, Characterized by Combined X-ray and Neutron Reflectometry
title_fullStr Neural Stem Cell Spreading on Lipid Based Artificial Cell Surfaces, Characterized by Combined X-ray and Neutron Reflectometry
title_full_unstemmed Neural Stem Cell Spreading on Lipid Based Artificial Cell Surfaces, Characterized by Combined X-ray and Neutron Reflectometry
title_short Neural Stem Cell Spreading on Lipid Based Artificial Cell Surfaces, Characterized by Combined X-ray and Neutron Reflectometry
title_sort neural stem cell spreading on lipid based artificial cell surfaces, characterized by combined x-ray and neutron reflectometry
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5445775/
https://www.ncbi.nlm.nih.gov/pubmed/28883366
http://dx.doi.org/10.3390/ma3114994
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