Standard Dyes for Total Protein Staining in Gel-Based Proteomic Analysis

Staining of two-dimensional gels is a primary concern in proteomic studies using two-dimensional gel electrophoresis with respect to the number of proteins analyzed, the accuracy of spot quantification and reproducibility. In this review article, the efficiency of the most widely used dyes was inves...

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Autor principal: Chevalier, François
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2010
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5445784/
https://www.ncbi.nlm.nih.gov/pubmed/28883353
http://dx.doi.org/10.3390/ma3104784
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author Chevalier, François
author_facet Chevalier, François
author_sort Chevalier, François
collection PubMed
description Staining of two-dimensional gels is a primary concern in proteomic studies using two-dimensional gel electrophoresis with respect to the number of proteins analyzed, the accuracy of spot quantification and reproducibility. In this review article, the efficiency of the most widely used dyes was investigated. Visible dyes (Coomassie blue and silver nitrate), fluorescent dyes (Sypro Ruby, Deep Purple) and cyanine labeled methods were compared.
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spelling pubmed-54457842017-07-28 Standard Dyes for Total Protein Staining in Gel-Based Proteomic Analysis Chevalier, François Materials (Basel) Review Staining of two-dimensional gels is a primary concern in proteomic studies using two-dimensional gel electrophoresis with respect to the number of proteins analyzed, the accuracy of spot quantification and reproducibility. In this review article, the efficiency of the most widely used dyes was investigated. Visible dyes (Coomassie blue and silver nitrate), fluorescent dyes (Sypro Ruby, Deep Purple) and cyanine labeled methods were compared. MDPI 2010-10-20 /pmc/articles/PMC5445784/ /pubmed/28883353 http://dx.doi.org/10.3390/ma3104784 Text en © 2010 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Review
Chevalier, François
Standard Dyes for Total Protein Staining in Gel-Based Proteomic Analysis
title Standard Dyes for Total Protein Staining in Gel-Based Proteomic Analysis
title_full Standard Dyes for Total Protein Staining in Gel-Based Proteomic Analysis
title_fullStr Standard Dyes for Total Protein Staining in Gel-Based Proteomic Analysis
title_full_unstemmed Standard Dyes for Total Protein Staining in Gel-Based Proteomic Analysis
title_short Standard Dyes for Total Protein Staining in Gel-Based Proteomic Analysis
title_sort standard dyes for total protein staining in gel-based proteomic analysis
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5445784/
https://www.ncbi.nlm.nih.gov/pubmed/28883353
http://dx.doi.org/10.3390/ma3104784
work_keys_str_mv AT chevalierfrancois standarddyesfortotalproteinstainingingelbasedproteomicanalysis

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